Peanut sclerotium blight greenhouse seedling inoculation method
A technique for white silkworm disease and white silkworm pathogen, which is applied in the field of peanut white silkworm inoculation at the seedling stage in greenhouse, and achieves the effects of simple inoculation operation, stable effect and small environmental impact.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0027] A method for seedling inoculation of peanut white silkworm in a greenhouse, comprising the steps of:
[0028] 1). Obtaining the white silkworm strain (isolation and obtaining the white silkworm bacteria): Collect the white silkworm sclerotia from the field peanut white silkworm strain in Wuhan, separate and purify it in the room {inoculate the PDA medium after surface disinfection, and keep the temperature at 30°C After culturing, pick the tip of the colony hyphae and transfer it to fresh PDA medium}, identify {collect the hyphae to extract DNA, then use the fungal DNA ITS interval universal primers ITS1 and ITS4 to PCR-amplify the sequence, and compare it in GenBanK by BLAST, and identify it as Sclerotium rolfsii}; obtain the bacterial strain of Sclerotium rolfsii;
[0029] 2). Preparation of bacteria-carrying inoculum: Activation of white silkworm: the sclerotia of white silkworm strain (white silkworm Wuhan strain) preserved at 4°C was surface-sterilized with 70wt% e...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com