Peanut sclerotium blight greenhouse seedling inoculation method

Abstract

The invention relates to a peanut sclerotium blight greenhouse seedling inoculation method. The peanut sclerotium blight greenhouse seedling inoculation method is characterized by comprising the following steps: 1) obtaining a sclerotium blight strain, namely collecting sclerotium blight sclerotia from the sclerotium blight strain in the field in Wuhan, separating and purifying indoors, carrying out sequence determination and comparison, and identifying Sclerotium rolfsii; 2) preparing inocula carrying bacteria, namely preparing a sclerotium blight bacterium dish, preparing a toothpick carrying the bacteria, and preparing oat granules carrying the bacteria; 3) inoculating the inocula carrying the bacteria to peanut seedlings in different ways; 4) inoculating different quantities of oat granules carrying the bacteria to the peanut seedlings; 5) inoculating the oat granules carrying the bacteria to the peanut seedlings in different seedling ages; and 6) inoculating the oat granules carrying the bacteria to different peanut varieties/lines. The invention establishes a simple and reliable sclerotium blight bacterium inoculation method and is convenient for seedling screening of peanut germplasm resistant to sclerotium blight, a material resistant to the sclerotium blight is obtained, and a technical means is provided for disease resistance breeding.

Description

technical field [0001] The invention relates to a seedling stage inoculation method of peanut white silkworm in greenhouse. Background technique [0002] Peanut canker sores, also known as white canker sores, is a soil-borne vascular disease caused by the genus Sclerotiumrolfsii Sacc. It occurs widely in the southern provinces and sporadically occurs in the northern peanut production areas. In recent years, with the warming of the global climate, the microenvironment of peanut canopy closed under the high-water-fertilizer and high-density planting mode has caused the damage of white silk disease to become more and more serious in our country, and the occurrence area has spread from the south to the north, causing serious economic losses. . In 2004, the diseased area in Linyi, Shandong Province reached 60,000 hectares, accounting for 40% of the city's sown area, and the rate of diseased plants in seriously diseased fields reached 67.3%. In 2007, the incidence rate of serio...

AI Technical Summary

Problems solved by technology

The most economical and effective way to prevent and control peanut canker sores is to plant disease-resistant varieties. There are no reports of high-resistant varieties in my country, and a stable and reliable resistance identification technology must be established to screen resistant varieties.

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