179results about How to "Improve inoculation efficiency" patented technology

The invention relates to a breeding method of pyemotes zhonghuajia, comprising wild pyemotes zhonghuajia collected from persimmon smotylon japonicam lesne imago or stenhomalus taiwanus matsushita larva is propagated by using scolytus seulensis musayawa larva or pupa, stenhomalus taiwanus matsushita larva or early age of pupa as breeder seed; under the proper condition, greater wax moth larva, lasioderma serricorne larva, the early age of pupa, early age of pupa of martianus dermestoides chevrolat or gelechiid can be taken as substitutional host, and the breeder seed is inoculated into the substitutional host as stock seed; the gelechiid, sitophilus zeamais, rice weevil larva, the early age of pupa or the greater wax moth larva can be taken as substitutional host, and the stock seed and the substitutional host can be inoculated according to the proportion of 1:3; under the proper condition, the pyemotes zhonghuajia can be cultured and bred. The method has the advantages of simple operation, low production cost, being suitable for large scale batch production and long-distance transportation and the like, and the bred pyemotes zhonghuajia can be applied to the biological control of disguised and boring pests.

The invention relates to a diphasic porous three-dimensional cell culture scaffold which is formed by a coarse fiber phase and a fine fiber phase which are entirely different in diameter. The coarse fiber phase is larger than cultured cells in size while the fine fiber phase is smaller than the cultured cells in size, the coarse fiber phase comprises multilayer coarse fiber structures, each two adjacent coarse fiber structures are arranged according to a certain angle, and the fine fiber phase is individually combined on one side or multiple sides of the coarse fiber phase and is averagely or intensively distributed in hole structures of the three-dimensional cell culture scaffold formed by the coarse fiber phase. In the diphasic porous three-dimensional cell culture scaffold, the fine fiber phase is much smaller than the cells in diameter, so that the cells can attach to nanofibers quite easily, and differentiation of stem cells on the nanofibers can be effectively promoted. Therefore, by means of adding the fine fibers on the three-dimensional cell culture scaffold, cell vaccination efficiency can be improved, and the cells grown on the fine fibers, particularly growth and differentiation of the stem cells, can be promoted and regulated.

The invention relates to the medicine detection field, and particularly relates to a disposable microorganism sample inoculating cup and its inoculating method and a phlegm washing method. The disposable microorganism sample inoculating cup has the inoculating and phlegm washing functions at the same time; the cup is simple in structure and convenient to operate. The invention further provides an inoculating method and a phlegm washing method by using the inoculating cup, thus the comprehensive and automatic control of the inoculating process is realized, the sampling and inoculating are more accurate, and the inoculating efficiency is higher; meanwhile, experimenters are free from direct contact with experimental samples, thus the infection risk of experimenters is greatly reduced; meanwhile, the phlegm washing method realizes the automatic control of the phlegm sample cleaning, thereby removing impurity disturbance for the next experiment.

The invention discloses paecilomyces lilacinus and a scale preparation method thereof, belongs to the technical field of microbial agricultural pesticide fertilizer, and particularly relates to a bacterial strain of the paecilomyces lilacinus of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 5339. The scale preparation of the paecilomyces lilacinus is realized by adopting a way of combining liquid state fermentation with solid state fermentation for the strain, namely, through four steps of the liquid state fermentation, the sterilization of a solid state fermentation substrate, the spray inoculation of the solid state fermentation substrate, and the culture of the substrate after the inoculation. The method has the advantages of high fermentation efficiency, short preparation period, simple preparation method and low preparation cost, the wide application of the paecilomyces lilacinus to agricultural disease control can be realized, and the actual demands of the high-efficiency and scale control of nematodes can be met.

The invention relates to a special rhizobium-containing controlled release fertilizer for soybean as well as preparation and application thereof. The fertilizer contains the following ingredients by weight parts: 10-25 parts of urea coated nitragin compound, 5-15 parts of sulphurated macromoleclar polymer coated urea, 8-18 parts of 5% humic acid coated urea, 35-40 parts of diammonium phosphate and 20-30 parts of potassium chloride. The invention further provides a preparation method of the special controlled release fertilizer for soybean. The dosages of the potash fertilizer, the phosphatic fertilizer and the enveloped nitrogenous fertilizer with the nutrients released at different periods are reasonably selected according to the rule of requirement of fertilization of soybean to form the special fertilizer with the double functions of inoculation and fertilization for soybean. Field experiments show that the utilization efficiency of the fertilizer can be obviously improved, and theyield of the soybean can be obviously increased.

The invention provides a multivalent immunogenic composition containing enterovirus antigens. The composition comprises inactivated EV71 antigens and/or inactivated CA16 antigens, and inactivated polio antigens. The composition can further comprise antigens selected from hepatitis A antigens, hepatitis B antigens, acellular pertussis antigens, tetanus toxoid, diphtheria toxoid, Haemophilus influenzae type b capsular polysaccharide, and meningococcal polysaccharide antigens, as well as physiologically acceptable carriers combined with bacterial polysaccharide antigens. The invention also provides a preparation method of the composition. The composition can prevent invasion of a plurality of pathogens simultaneously without interference among the antigens, and the immunogenicity is no less than that of individually activated antigens. With the composition, vaccination processes are significantly simplified, and the vaccination efficiency is improved with reduced costs.

The invention relates to the field of cultivation of edible mushrooms, in particular to edible mushroom inoculation equipment. The edible mushroom inoculation equipment comprises a rack, a conveying belt arranged on the rack, a driving motor driving the conveying belt to circularly move through transmission parts, as well as tension devices used for adjusting the degree of tightness of the conveying belt, wherein a punching device, a sizing device, a film sealing device, a film cutting device and a film pressing device are sequentially arranged on the rack in the conveying direction of the conveying belt; a mushroom stick loading station is arranged on the upstream of the punching device, an inoculation station is arranged between the punching device and the sizing device, and an unloading station is arranged on the downstream of the film pressing device. According to the edible mushroom inoculation equipment, an assembly line is adopted, edible mushrooms can be cultivated in an assembly line manner, procedures including punching of mushroom sticks, sizing, film sealing and the like can be completed automatically except loading, inoculation and unloading procedures required to be performed manually, accordingly, the inoculation efficiency of the edible mushrooms is greatly improved, the yield is increased, the productivity is liberated, and the labor intensity of workers is reduced.

The invention discloses an integrated dynamic inoculation culture method for an osteogenous seed cell and a matched perfusion type bioreactor thereof. A peristaltic pump, a perfusion chamber, a liquid storage bottle, a channel converter and a connection tube constitute a double loop structure for perfusion inoculation and perfusion culture. When the double loop structure is used, two loops are switched by regulating the channel converter, thereby achieving the effect of integrated dynamic inoculation and dynamic culture. The dynamic inoculation and dynamic culture of the cell are carried out through the perfusion type bioreactor, thereby enhancing the inoculation efficiency and uniformity of the cell, enlarging the range and efficiency of supplying nutrition and oxygen and simultaneously providing the shearing strength stimulus to the osteogenous cell so that the inoculation and culture of the cell are integrally carried out in the same environment, thus omitting a conversion link. Compared with the existing cell inoculation and culture technology, the invention has the advantage that a proper environment is provided for adhesion, distribution and differentiation of the cell and tissue construction, thereby obviously enhancing the effect of tissue engineering bone construction.

The invention discloses a method for identifying and evaluating wheat scab expansion resistance. The method is mainly characterized in that after wheat ears, gibberella spore liquid is injected into internode stems below ears and not dropped into florets, and the wheat scab expansion resistance is comprehensively evaluated according to occurring time of a first disease spikelet and the rate of the disease spikelet in the 22-25th day after inoculation. The method has the greatest advantages that moisturizing measures are omitted after inoculation in a large field or greenhouse environment, and the method solves the problem that a traditional single flower dripping method needs the moisturizing measures such as bagging or spraying and water spraying for identification. Compared with the traditional single flower dripping method, the method is higher in inoculation and lower in cost, disease prevalence rate is decreased, phenotypic difference resisting and sensing capacity of varieties is maximized, and common technicians can more effectively judge the scab expansion resistance of wheat varieties.

The invention provides a method and device for preparing a bone marrow mesenchymal stem cells-tube scaffold compound. In the invention, a cylinder in the inner center of a cylindrical groove blocks a central channel (inner hole) of a tube scaffold, so that culture fluid flowing through the culture fluid inlet on a top cap can completely flow across the outer wall pores of the tube scaffold, thus ensuring that cells in the outer wall pores of the tube scaffold acquire nourishment of the culture fluid. The method provided by the invention improves the inoculation efficiency of the bone marrow mesenchymal stem cells in the PCL (polycaprolactone) tube scaffold, and improves the uniform distribution and amplification capacities of the cells in the outer wall of the tube scaffold, a compound tube scaffold with bone marrow mesenchymal stem cells of even and high-density distribution can be obtained by the method, and the method is hopeful to be applied to repair of tube tissue damage clinically.

The invention relates to a peanut sclerotium blight greenhouse seedling inoculation method. The peanut sclerotium blight greenhouse seedling inoculation method is characterized by comprising the following steps: 1) obtaining a sclerotium blight strain, namely collecting sclerotium blight sclerotia from the sclerotium blight strain in the field in Wuhan, separating and purifying indoors, carrying out sequence determination and comparison, and identifying Sclerotium rolfsii; 2) preparing inocula carrying bacteria, namely preparing a sclerotium blight bacterium dish, preparing a toothpick carrying the bacteria, and preparing oat granules carrying the bacteria; 3) inoculating the inocula carrying the bacteria to peanut seedlings in different ways; 4) inoculating different quantities of oat granules carrying the bacteria to the peanut seedlings; 5) inoculating the oat granules carrying the bacteria to the peanut seedlings in different seedling ages; and 6) inoculating the oat granules carrying the bacteria to different peanut varieties/lines. The invention establishes a simple and reliable sclerotium blight bacterium inoculation method and is convenient for seedling screening of peanut germplasm resistant to sclerotium blight, a material resistant to the sclerotium blight is obtained, and a technical means is provided for disease resistance breeding.

The present invention discloses a method of cultivating edible fungus and its special tools. The method includes such steps as supplying material, presetting a inoculating tube, sterilizing, inoculating, management of growing and fruiting, firstly filling mixed culture medium into a bag and stacking the bag on a cultivating frame, then inserting the inoculating tube into a planting bag, whereafter sealing and covering the heat preservation layer with a plastic film, inputting wet and hot steam for sterilizing the planting bag, inoculating after sterilization, when it is inoculating, fungus seeds were filled into the inoculating tube and contact with the culture medium through tube holes of the inoculating tube then finish inoculating operation, the management of growing and fruiting was operated by normal method. The present invention has incorporated sterilizing and inoculating operation of edible fungus culture medium bag without moving and displacing the planting bag, i.e. inoculating could be operated in situ in axenic circumstance after sterilizing, inoculating operation has finished after fungus seeds were filled into the inoculating tube. The present invention can result in effects of growing quickly etc.

The invention relates to a method for screening tobacco plants resistant to tomato spotted wilt virus, belonging to the field of plant protection technology. The method includes five steps: nicotianabenthamiana seedling raising, preparation of resistance identification for tobacco seedlings, preparation of inoculation buffer, inoculation of tobacco seedlings and identification of disease resistance. The principle of the invention is that before the tobacco disease resistance identification is carried out, the field tomato spotted wilt virus source is activated on the nicotianabenthamiana, and the diseased tissue of the tobacco is collected as a poison source to carry out disease resistance identification.

The invention discloses a technology for producing greasy crystallized honey. The technology comprises the following steps of 1, a seed preparation comprising mixing rape honey, clean water and micro-grinded bee pollen, stirring to obtain a seed mixed solution, heating rape seed raw material honey in a water-bath, adding the heated rape seed raw material honey into the seed mixed solution with stirring, filtering by a sieve of 60 meshes, pouring the filtrate into a wide-mouth container, and putting the wide-mouth container into an inclosed thermostatic chamber having a temperature of 15 to 20 DEG C for 10 to 15 days to obtain crystal seeds, 2, a raw material honey selection process comprising selecting all natural honey having a light color, low water content less than 20% and high glucose content more than 35% as a raw material for production of the greasy crystallized honey, and 3, a raw material honey inoculation and crystallization process comprising a, inoculation, b, pre-crystallization, and c, after-crystallization. The greasy crystallized honey obtained by the technology has white and smooth appearance, a fine taste, and good stability, plasticity and repeatability.

An industrialized mushroom production method comprises the following steps of making wood scraps; mixing materials; packaging the materials into bags; sterilizing the materials; feeding fungus barrels to a sterilizing chamber and sterilizing the fungus by using steam; directly feeding the fungus barrels to an inoculation workshop after the fungus barrels are sterilized; performing inoculation on the fungus barrel; and fruiting. Industrialized mushroom production is realized by the steps. The industrialized mushroom production system comprises a scrap making device, a weighing and mixing device, a fungus barrel packaging device, a sterilizing chamber, an inoculation workshop and a fruiting workshop, wherein the scrap making device is connected with the weighing and mixing device through a conveying device; the weighing and mixing device is connected with the fungus barrel packaging device through the conveying device; a first sealing door and a second sealing door are respectively arranged at two ends of the sterilizing chamber; the first sealing door is close to the fungus barrel packaging device as much as possible; and the second sealing door is directly positioned in the inoculation workshop. The probability that the fungus barrels are infected by infectious microbes is reduced in a production process, the inoculation efficiency is improved, and the labor intensity is reduced. Moreover, the utilization rate of equipment is improved, and continuous production is realized.

The invention belongs to the technical field of biology, and particularly relates to a plant tissue culture support. The support comprises dry sphagnum and de-ionized water, wherein the volume ratio of the dry sphagnum to the de-ionized water is (0.5-1):1. Compared with the prior art, the plant tissue culture support has the beneficial effects that the plant tissue culture support is easy to operate and low in tissue culture cost; energy resources are saved; the working time is shortened, the labor intensity is reduced, the inoculation efficiency is improved, and the pollution probability is reduced; an explant grows robustly, and is high in transplantation survival rate; good fixing effects are achieved; the air permeability is high; a culture medium is long in service cycle; a tissue culture environment can more meet physiological requirements of a plant.

The invention relates to an edible fungus producing apparatus, in particular to a simple liquid strain inoculation device which particularly structurally comprises a glass bottle, an air pump, an inflating gun, an air inlet pipeline, a bottle stopper and a liquid strain circulating pipeline. The air pump is connected with the air inlet pipeline by a rubber pipe and air filters, the inflating gun is connected with the liquid strain circulating pipeline by a rubber pipe, the bottle stopper is fixed into a bottle opening of the glass bottle, and the air inlet pipeline and the liquid strain circulating pipeline respectively penetrate through the bottle stopper and are arranged inside the glass bottle. During inoculation, liquid strain in the bottle is pressed out of the liquid strain circulating pipeline under the action of air pressure, and is connected into culture materials under a control effect of the inflating gun. The simple liquid strain inoculation device is simple in structure, is efficient and speedy and is high in inoculation efficiency when applied to liquid strain inoculation, and is extremely low in infection rate.

The invention discloses a method for artificially inoculating pine wood nematodes by a semiannual masson pine seedling tender tip skin scratch and an application thereof. The method comprises the following steps of: (1) preparing living pine wood nematodes into a pine wood nematode suspension; (2) selecting a tender tip position 3-5cm below the tip of a semiannual masson pine seedling, pulling outneedle leaves, and longitudinally scratching two or more wounds at the skin of the tender tip of the masson pine from which the needle leaves are pulled out; (3) enclosing a small water-tight funnelat the part where the needle leaves are removed by using a sealing film; wherein the lower part of the small funnel clings to the tender tip of the masson pine and is flush with the lower part of thewounds; (4) injecting the pine wood nematode suspension into the small funnel to inoculate the pine wood nematodes; and (5) placing the semiannual masson pine seedlings inoculated with the pine wood nematodes in an outdoor environment for cultivation. The method is suitable for artificially inoculating pine wood nematodes to the semiannual masson pine seedlings, can effectively reduce production cost, and has wide application prospect and important production significance.

The invention discloses an inoculation identification method for clubroot of cruciferous crops, belongs to the technical field of plant protection, and provides the inoculation identification method for clubroot of cruciferous crops. Aseptic dried sphagnum is used as a carrier for culturing Plasmodiophora by inoculating. The method of the invention has the advantages that the consumption of bacterial sources is low, inoculating efficiency is high, operability is high, pathogenesis is uniform, result stability is good, disease determination is highly sensitive, the method is applicable to the identification of physiological races of small samples and the identification of disease resistance in large-batch varieties, and the method serves for breeding novel disease-resistant varieties and reasonably laying out disease-resistant varieties.

The invention relates to the technical field of shiitake mushroom production, and in particular relates to a stick type summer culture method for shiitake mushrooms and a rapid inoculator. The culturemethod comprises the following steps: preparation of a culture medium, bagging, sterilizing, inoculating, thalli culturing, coloring management, mushroom discharging and harvesting and the like. Themethod has the advantages of being low in planting cost, good in quality of shiitake mushrooms and short in production period. In addition, the inoculator provided by the invention is simple in structure and convenient to use and improves the work efficiency greatly.