31results about How to "Improve inoculation efficiency" patented technology

The utility model belongs to the technical field of lentinus edodes liquid strain inoculation, and particularly relates to a lentinus edodes liquid strain inoculation device which comprises a base, a containing base used for containing a fungus bag is fixed to the top of the base, two first air cylinders are symmetrically fixed to the top of the base, and L-shaped baffles are fixed to the ejection ends of the first air cylinders. The L-shaped baffle is slidably connected to the top of the base, a vertical plate is fixed to the top of the base, and a fixing plate is fixed to the front face of the vertical plate. Through the arrangement of the placing seat, the first air cylinder and the L-shaped baffle plate, the mushroom bag can be more conveniently stabilized, through the arrangement of the vertical plate, the fixed plate, the second air cylinder, the mounting plate, the multi-branch inoculation pipe, the storage box, the water pump, the liquid pumping pipe and the liquid feeding hose, the mushroom liquid strain can be more conveniently and quickly inoculated on the mushroom bag, and through the arrangement of the stirring assembly, the mushroom liquid strain can be more conveniently and quickly inoculated. The mushroom liquid strain inoculation device is simple in structure, capable of preventing the mushroom liquid strain from precipitating, good in overall using effect, time-saving and labor-saving in operation and high in inoculation efficiency.

The utility model discloses a full-automatic vaccine inoculation device which comprises a feeding device, an inoculation device and a control device. The disinfection device comprises a disinfection assembly and a disinfection conveying line, and the disinfection assembly is arranged on the upper side of the disinfection conveying line; the feeding device is used for transferring the disinfected egg trays; the inoculation equipment comprises an inoculation conveying line and an inoculation module, and the inoculation module is provided with an inoculation needle; and the buffer conveying line is used for transferring and temporarily storing the inoculated egg trays. The full-automatic inoculation machine has the advantages that full-automatic inoculation production is achieved, feeding, disinfection, shell breaking and poison injection are completed, production efficiency is high, and production quality is guaranteed.

The utility model relates to the technical field of morchella esculenta cultivation, in particular to a strain inoculation device for morchella esculenta cultivation, which comprises a gun body and an inoculation needle, a sliding component is arranged in the gun body, a liquid inlet pipe is fixedly connected onto the gun body, a one-way valve is arranged on the liquid inlet pipe, one side of the gun body is fixedly connected with a fixing ring, and the other side of the gun body is fixedly connected with the inoculation needle. The fixed ring is arranged on the outer side of the inoculating needle in a sleeving manner; one side of the gun body is connected with a limiting ring through two telescopic rods, the limiting ring is provided with two symmetrically-arranged mounting cavities, an insertion rod is slidably mounted in each mounting cavity, and one end of each insertion rod is connected with the corresponding inoculation needle in a clamped mode. According to the inoculation gun, the inoculation needle is convenient and rapid to replace, the phenomenon of thread slipping cannot occur after multiple times of replacement, normal use of the inoculation gun is facilitated, and then the inoculation efficiency of morchella liquid strains is high.

The utility model provides a veterinary vaccination fixing device which comprises a mounting bottom plate, fixing side plates are fixedly connected to the tops of the two ends of the mounting bottom plate, a vaccination cavity is formed in the middle of each fixing side plate, mounting pieces are arranged at the tops of the two ends of each fixing side plate, a rotating block is arranged on a mounting rod, and the rotating blocks are fixedly connected with the fixing side plates. A protective cover plate is fixedly connected to the inner wall of the side end of the rotating block, clamping rods are arranged on the inner walls of the two ends of the protective cover plate, clamping grooves are formed in the tops of the two ends of the mounting side plate, a locking plate is arranged on the tops of the middles of the clamping grooves, and fastening bolts are arranged on the inner walls of the two ends of the locking plate. According to the veterinary vaccination fixing device provided by the utility model, the clamping rod arranged at the side end of the protective cover plate is matched with the clamping groove for use, so that the protective cover plate can be tightly locked, an animal is prevented from panic during vaccination, and the vaccination fixing device is convenient to use during vaccination. And an extrusion plate arranged in the device can be used for temporarily limiting and fixing the animals.

This invention discloses a biocontrol spore cultivation device and its usage method for controlling Japanese pine scale, belonging to the field of pine scale control technology. The device includes an inoculation unit and several cultivation units. The inoculation unit includes a frame with a chain conveyor inside. Multiple positioning support plates are evenly spaced on the chain conveyor. A pre-compression mechanism and an inoculation mechanism are located directly above the chain conveyor on the frame, both spanning the chain conveyor. Several cultivation units are located at the outlet of the inoculation unit. This invention achieves automatic inoculation of biocontrol spores, improving inoculation efficiency and enabling precise control of the inoculation amount, thus improving the uniformity and consistency of biocontrol spore concentration. Next, the inoculated bottles are transferred to multiple cultivation units for static cultivation to improve the viability and yield of biocontrol spores, meeting the needs of large-scale production.

The utility model provides a detachable inoculation tool. The detachable inoculation tool comprises: a handle; a groove, wherein the groove is formed in the handle; the battery bin is arranged on the handle; the sleeve pipe is fixedly arranged on the handle; the extension rod is installed on the inner wall of the sleeve in a sliding mode, and a screw hole is formed in the bottom end of the extension rod; the length adjusting mechanism is arranged on the groove, and the length adjusting mechanism is used for adjusting the length of the inoculation tool; the mounting and connecting mechanism is arranged at the top end of the extension rod, and the mounting and connecting mechanism is used for mounting an inoculation mechanism. The detachable inoculation tool has the advantages that the length of the inoculation tool can be adjusted, the angle of the inoculation end can be adjusted conveniently, and operation is convenient.

The utility model relates to the technical field of auxiliary equipment, in particular to an auxiliary device convenient for vaccination, which comprises two bases, moving mechanisms are arranged on the two bases, and the moving mechanisms are connected with four clamping plates; the moving mechanism comprises four grooves formed in the base, moving blocks are arranged in the four grooves in a sliding mode, connecting mechanisms are arranged between the four moving blocks and the four clamping plates, two bidirectional screw rods are rotatably installed on the base, the two threaded ends of the two bidirectional screw rods are in threaded connection with the moving blocks, and the two ends of the two bidirectional screw rods are in threaded connection with the moving blocks. And a driving mechanism is arranged between the two bidirectional screw rods. According to the device, the two-way screw drives the moving block to move on the base, the moving block drives the clamping plate to clamp the arm of a child, so that the arm of the child is clamped, the clamping force is not too large, the child does not feel painful in the extrusion process of the arm of the child, and medical staff can conveniently vaccinate the child.

The utility model discloses a perforated plate inoculation instrument. The porous plate inoculation instrument comprises a rotary uniform mixing sample tube mechanism and an alternate uncovering mechanism, wherein the rotary uniform mixing sample tube mechanism comprises a fixed base plate, a support frame, a rotary fixed frame, a rotating wheel, a driving structure and a sample tube frame. According to the perforated plate inoculation instrument provided by the embodiment of the invention, the full-process automatic operation is realized by arranging the rotary uniform mixing sample tube mechanism and the alternate uncovering mechanism, and the rotary uniform mixing sample tube mechanism comprises the fixed base plate, the supporting frame, the rotary fixed frame, the rotating wheel, the driving structure and the sample tube frame; the driving structure can be used for driving the rotating wheel to rotate so that the fixing plate can turn over the sample tube frame, the rotating speed of the sample tube frame is increased, the uniform mixing treatment efficiency of sample tubes arranged on the sample tube frame is improved, the cover opening and liquid pumping speed is increased in cooperation with the alternate cover opening mechanism, and the inoculation efficiency is improved.

The present invention discloses a vaccination vehicle with a telescopic isolation cabin, which includes a vehicle body, a vaccination table and a telescopic isolation cabin, wherein the vaccination table is arranged in the vehicle body, a vaccination window is arranged on the side wall of the vehicle body at a position opposite to the vaccination table, and the telescopic isolation cabin is arranged outside the vehicle body opposite to the vaccination window; the telescopic isolation cabin has a drawer-type supporting base, a folding shed and a folding shed storage cabin, and the folding shed is lowered to form an independent vaccination space, which can separate the vaccination area from the medical area, reduce the risk of cross infection, and the vaccination work efficiency is high.

The invention discloses a biological fermentation feed inoculation device which comprises an inoculation tank, a feed inlet connected to the top of the inoculation tank and used for feeding and a stock solution tank used for storing inoculation liquid, and the stock solution tank is fixedly connected to the left side of the inoculation tank; the biological fermentation feed inoculation device further comprises a first shaft rod which transversely penetrates through and is rotationally connected to the interior of the feeding opening, a buffer plate is rotationally connected to the top of the inner side of the feeding opening, connecting rods are fixedly connected to the outer side of a second shaft rod at equal intervals, and a bottom groove is formed in the bottom of the inoculation tank in a communicating mode. The material inoculation quality can be improved, and the inoculation efficiency can be improved. The invention also discloses a preparation method of the biological fermentation feed, and the preparation method of the biological fermentation feed is used for fermenting the straw material, so that the nutritional value and the utilization rate of the straw can be improved.

The utility model discloses an edible fungus inoculation gun, particularly relates to edible fungus inoculation technical field, including the frame subassembly, the frame subassembly is equipped with a plurality of inoculation subassembly in the sliding mode, the frame subassembly includes the mounting plate, the obverse side of mounting plate is equipped with the first chute, the middle part of mounting plate bottom surface is fixedly equipped with the grip, and the handle is equipped with the second chute. According to the edible mushroom inoculation gun, edible mushroom shred blocks located in the inoculation barrel can be pushed into the inoculation positions by controlling the piston abutting head to move, so that synchronous inoculation can be rapidly carried out on multiple inoculation positions, the inoculation efficiency is effectively improved, the inoculation difficulty is reduced, practicability is higher, the inoculation interval can be further adjusted according to needs, and the practicability is high. The positions of the inoculation barrels are manually adjusted according to needs so that the inoculation barrels can be aligned with the respective inoculation positions, after alignment, the abutting screws are screwed, the bottom ends of the abutting screws abut against the inner walls of the abutting grooves, then position adjustment of the inoculation barrels can be completed, and adaptability is higher.

The utility model relates to the technical field of strain inoculation devices, and discloses a wild mushroom strain inoculation device which comprises a device body, four rotating shafts are rotationally arranged on one side in the device body, gears are fixedly arranged in the four rotating shafts, tips are fixedly arranged on the sides, close to the outer sides, of the four rotating shafts, and the tips are fixedly arranged on the outer sides of the four rotating shafts. Four second compressed springs are fixedly arranged on one side of the interior of the device body, racks are fixedly arranged on one sides of the four second compressed springs, the exteriors of the four racks slide on one side of the interior of the device body, and the opposite sides of every two of the four racks are connected to the exteriors of the four gears in a meshed mode; and connecting blocks are fixedly arranged on the opposite sides of every two of the four racks. When the device is used, the device is inserted into a fungus bag through the pointed end, then the push block is pushed through the push rod, so that the pointed end is opened, inoculation of strains is facilitated, meanwhile, the feeding mechanism starts to automatically feed, and next-time strain inoculation is facilitated.

The utility model belongs to the technical field of fungus inoculation tools, and particularly discloses a fungus slant inoculation tool. The inoculation tool is used for solving the problems that spores cannot be collected thoroughly when being scraped by an existing inoculation tool, and the spores are easy to scatter. The fungus slant inoculation tool comprises a handle, a connecting rod and a scraping shovel, the front end of the handle is fixedly connected with the rear end of the connecting rod, and the front end of the connecting rod is connected with the middle position of the rear end of the scraping shovel; the scraping shovel comprises a shovel face and blocking pieces, the blocking pieces are arranged on the periphery of the shovel face, the front end of the shovel face is concaved inwards to be in a crescent shape, a gap is reserved between the blocking piece located on the front side of the shovel face and the front end edge of the shovel face, the vertical distance between the lower edge of the blocking piece located on the front side of the shovel face and the crescent shovel face is 1-2 mm, and the upper edges of the blocking pieces are flush with one another. According to the utility model, the crescent shovel surface is matched with the baffle plate positioned on the front side of the shovel surface, so that the spores on the inclined surface can be quickly scraped and collected, the spores are prevented from overflowing and scattering, and infectious microbe pollution and strain waste are reduced.

The utility model discloses a full-automatic immunization inoculation machine for poultry embryos. The full-automatic immunization inoculation machine comprises a rack, and a propelling device, an egg sterilizing device, an air drying device and an inoculation device which are sequentially arranged along the rack, the propelling device synchronously pushes the egg tray to move through the shifting fork and the push-pull shaft; the egg sterilizing device sprays disinfectant through an air pressure tank and a mixed spraying cavity; the air drying device achieves sterile air drying through a high-efficiency filter and a static pressure box. The inoculation device adopts two groups of shell breaking needles and inoculation needles, the inoculation needles penetrate through the hollow shell breaking needles to complete injection after shell breaking, the other group of needles are soaked and disinfected in a disinfectant pipe, and residual liquid is removed through a vacuum generator. According to the utility model, full-automatic disinfection, shell breaking, inoculation and needle disinfection are realized, the efficiency is high, and cross contamination is avoided. And a laminar flow cover and an egg lifting device can be selected to further improve the cleanliness and the positioning precision. The method is suitable for large-scale immunization of poultry embryos.

The present invention discloses a clinical-grade batch umbilical cord tissue inoculation device, which relates to the field of biotechnology and includes a connecting hose and a folding inoculation plate. The folding inoculation plate includes a first folding plate and a second folding plate hinged to the first folding plate, and a plurality of discharge pipes are arranged on the first folding plate and / or the second folding plate, and an inoculation hole is arranged at the end of any discharge pipe; the discharge end of the connecting hose is connected to the folding inoculation plate, and the head end of any discharge pipe is connected to the connecting hose; the feed end of the connecting hose is used to introduce umbilical cord tissue, and the umbilical cord tissue reaches the inoculation hole in turn through the connecting hose and the discharge pipe, and forms an inoculation tissue block in the inoculation hole. The present invention realizes clinical-grade, high-efficiency, batch inoculation tissue blocks, and the formed tissue blocks are flat, uniform in volume, neatly arranged, and standardized inoculation, and the umbilical cord tissue is not easy to lose moisture, the technology is simple, the operation is reliable, and the aseptic control of the production operation process is guaranteed, meeting the clinical mass production needs.

The invention relates to the technical field of agricultural planting, in particular to a Yunnan pine mycorrhizal seedling cultivation device and method.The device comprises a greenhouse, a plurality of seedling trays are arranged in the greenhouse, and the device further comprises an E-shaped support; the transverse end of the E-shaped support is sequentially, symmetrically and rotationally matched with sliding blocks, first connecting rods and fixing plates from top to bottom, and a first fixing shaft is fixedly connected between every two adjacent first connecting rods. A second fixing shaft is fixedly connected between every two adjacent fixing plates, triangular supports are hinged to the side walls of the fixing plates in the circumferential direction, a supporting plate is fixedly connected between every two adjacent triangular supports, and seedling trays are all placed on the supporting plates. An inoculation assembly for inoculating strains to the Yunnan pine root seedlings is arranged between every two adjacent sliding blocks; wherein one fixing plate is provided with a driving assembly, and the E-shaped support is provided with a monitoring assembly. The growth vigor of the Yunnan pine root seedlings can be automatically identified, and the Yunnan pine root seedlings are automatically inoculated when the Yunnan pine root seedlings are in the inoculation period, so that the mycorrhiza formation rate and the seedling survival rate are improved.

The invention belongs to the technical field of cell culture, and discloses a preparation method of a droplet microcarrier, which comprises the following steps: by taking a protein solution as a water phase and perfluorocarbon as an oil phase, dispersing the oil phase into the water phase by using a droplet printing device to form a microcarrier, and incubating to obtain the droplet microcarrier. The protein solution contains at least one protein with film-forming property and at least one protein which can be combined with a cell membrane surface receptor to form an adhesion site. According to the preparation method provided by the invention, a plurality of proteins are mixed for co-incubation, and a protein film composed of the plurality of proteins has more functionality and is more similar to an environment of an extracellular matrix, so that the prepared droplet microcarrier has higher inoculation efficiency and cell bearing capacity, can maintain cell phenotypes and functions, and can be used for preparing the liquid droplet microcarrier. And the method has stronger multiplication capacity and multipotency.

The utility model relates to a disinfection device for artificial propagation promotion of edible mushrooms, and belongs to the technical field of edible mushroom cultivation. The middle section of the bag material conveying device is located in a caulking groove in the working table, the protective cover is installed on the working table, the disinfectant spraying device is installed on the working table and connected with the disinfectant barrel and the disinfectant collecting barrel, the inoculation device is installed in the protective cover, and the pure air blowing device is installed at the top in the protective cover. An ultraviolet germicidal lamp is mounted at the top in the protective cover; the interior of the sterile inoculation chamber is sterilized and disinfected through an ultraviolet germicidal lamp; a clean environment is provided through the pure air blowing device, the purity in the inoculation chamber is guaranteed, and microorganisms and pollutants can be reduced to the maximum extent; the disinfectant spraying device can regularly and automatically sterilize and disinfect the inoculation rod, so that the inoculation success rate is increased; the inoculation success rate can be guaranteed, and propagation and growth of edible mushrooms are promoted; the inoculation efficiency is high, the manual labor intensity is reduced, and the labor cost is reduced.

The utility model relates to the field of veterinary instruments, and specifically discloses a fish vaccine continuous soaking inoculation device based on an Archimedes screw rod. The device comprises a fish delivery system, a vaccine soaking liquid delivery system, an Archimedes screw rod continuous soaking inoculation system, a vaccine soaking liquid recycling system, a movable screw rod rack and a drive motor box. The utility model promotes the fish school in the compartment to uniformly soak in the vaccine soaking liquid through screw rod rotation, improves the inoculation efficiency while improving the screw rod space utilization rate, and reduces errors caused by human factors and inoculation batches; the design of the vaccine soaking liquid recycling system circulates and utilizes the vaccine soaking liquid during the inoculation process, thereby reducing the overall system operation cost.

The utility model discloses an edible fungus inoculation clamping cover mechanism and an edible fungus inoculation device, and particularly relates to the technical field of edible fungus inoculation, the edible fungus inoculation clamping cover mechanism comprises a support, a frame is arranged in the support, two sets of clamping cover assemblies are arranged in the frame, each set of clamping cover assembly comprises two clamping plates symmetrically distributed in the front, and the clamping plates are arranged in the frame. Each clamping plate is provided with a plurality of semicircular grooves matched with an edible mushroom bag cover, sliding grooves are formed in the inner wall of the frame, the interiors of the sliding grooves in the two sides of the frame are rotationally connected with two bidirectional lead screws with the front ends and the rear ends fixed, and each bidirectional lead screw is in threaded connection with two nut blocks distributed front and back. According to the cover clamping mechanism, the multiple clamping plates are synchronously adjusted through the driving assembly to clamp covers stably, the whole cover clamping mechanism is simple in composition and low in failure rate and maintenance cost, the action of the cover clamping mechanism is more reliable and efficient, edible fungus bags are stabilized through the positioning mechanism, and therefore the cover clamping stability of the cover clamping mechanism can be further improved.

The utility model discloses a portable inoculation device, which belongs to the technical field of edible mushroom inoculation, and comprises a driving component, a connector is arranged on one side of the driving component, a rotating shaft is in transmission connection with the inner side of the connector, a charging barrel is mounted on one side of the rotating shaft, and the charging barrel is in transmission connection with the connector. One side of the charging barrel is fixedly connected with a tapping head, and the upper part of the charging barrel is in bolted connection with a hopper; a mounting bolt is arranged to penetrate through an arc-shaped clamp to be in threaded connection with a charging barrel, firstly, a strain to be inoculated is put into a hopper, at the moment, a handheld handle is held by one hand, a handle is held by the other hand, a tapping head abuts against a mushroom stick, and a switch is pressed, so that a motor and a rotating shaft drive a spiral auger to rotate, and the strain falls into the charging barrel from an inclined hopper; strain is conveyed to the discharging opening through the spiral auger and then enters the holes in the mushroom sticks, after inoculation of one mushroom stick is finished, another mushroom stick is selected for inoculation, and the whole structure is convenient to use, good in portability, high in inoculation efficiency and low in manual labor intensity.

The invention discloses an edible mushroom inoculation machine which comprises a rack, a conveying mechanism is arranged on the rack and used for clamping and conveying placed mushroom sticks, an inoculation mechanism is arranged above the rack and used for inoculating the mushroom sticks conveyed to the lower portion of the inoculation mechanism, the inoculation mechanism comprises a plurality of inoculation air cylinders fixed to the upper end of the rack, and inoculation heads are arranged at the lower ends of the inoculation air cylinders. The inoculation head is externally connected with liquid strains, and the liquid strains are injected into the mushroom sticks through downward movement of the inoculation air cylinder. The conveying mechanism is arranged to convey mushroom sticks to be uniformly inoculated to the position below the inoculation mechanism, an inoculation air cylinder of the inoculation mechanism is automatically controlled by the control system to move downwards, meanwhile, an inoculation head moves downwards and is inserted into the mushroom sticks to inject strains into the mushroom sticks to complete inoculation, and after inoculation is completed, the mushroom sticks are automatically conveyed out through the conveying mechanism; according to the inoculation machine, automatic inoculation is achieved, the inoculation efficiency is improved, meanwhile, the situation that disinfection is not thorough due to manual inoculation is avoided, and the survival rate of inoculated strains is increased.

The utility model relates to the technical field of hypsizygus marmoreus liquid strain culture, in particular to an inoculation device for strain cultivation, which comprises a support rod, an inoculation head connected to the left end of the support rod, a handle arranged at the right end of the support rod, a limiting rubber plug sleeved on the ring surface of the support rod, and a limiting baffle plate fixedly arranged on the ring surface of the support rod and close to the right end. By means of the design, the problems that in the hypsizygus marmoreus liquid strain culture process, hyphae are manually divided through an inoculation hook and transferred into a culture bottle, operation is tedious, the speed is low, and meanwhile the pollution risk is high are solved. According to the inoculation hook, the structural design of an original inoculation hook is improved, the inoculation process of a hypsizygus marmoreus liquid strain is greatly simplified, the inoculation speed and the inoculation efficiency are improved, meanwhile, hyphae of hypsizygus marmoreus can be prevented from being in contact with a bottle opening in the inoculation process, the pollution risk in the inoculation process is reduced, and the safety of the hypsizygus marmoreus is improved. The powerful technical support is provided for the culture of the hypsizygus marmoreus liquid strain.

The present invention relates to a novel coronavirus influenza immunogenic composition and a preparation method and application thereof. The novel coronavirus influenza immunogenic composition of the present invention contains a fusion protein comprising the RBD of the novel coronavirus S protein or a functionally active fragment thereof, and the NTD of the novel coronavirus S protein or a functionally active fragment thereof, and a human influenza virus immunogen comprising one or more human influenza virus hemagglutinin (HA) proteins. The immunogenic composition provided by the present invention and the novel coronavirus influenza combination vaccine prepared therefrom have good immunogenicity, can prevent infection by two viruses at the same time with one vaccination, are safe and efficient, have no immune interference, and there is no mutual inhibition between the two immunogenic component vaccines, and can be well compatible.

This invention discloses a cultivation process for open-field cultivation of Auricularia auricula-judae using long bags, comprising the following steps: Step 1: After mixing the granules and substrate, the granules and substrate are placed inside container one and then placed in container two for sterilization; Step 2: Container one is transferred to the interior of a sterile inoculation box, the original spawn is placed inside, and the granules and substrate are inoculated with the original spawn. After inoculation, container one is transferred to a spawn cultivation room for spawn cultivation; Step 3: The substrate for the spawn bags is packed inside the spawn bags to form spawn bags, which are then sterilized. The sterilized spawn bags are then transferred to the interior of a sterile inoculation box, and the granules and spawn bags are inoculated. The spawn bags are then placed on a square spawn bag cultivation rack for spawn cultivation; Step 4: A mesh-type spawn rack is constructed, and the spawn bags with spawn cultivation are transferred into the squares of the mesh-type spawn rack for spawn management until harvest. This cultivation process for open-field cultivation of Auricularia auricula-judae using long bags improves the quality of both the spawn bags and the Auricularia auricula-judae.

The utility model relates to the technical field of edible mushroom stick inoculation, in particular to an edible mushroom stick punching and inoculating all-in-one machine which comprises a working plate and a plurality of mushroom stick bodies, a plurality of supporting columns are fixed to the bottom end of the working plate, and a conveyor body arranged from left to right is installed on the top face of the working plate. A plurality of containing plates arranged from left to right are fixed to the top face of a conveying belt of the conveyor body. Through the arrangement of the electric push rod, the liquid passing box, the fixed pipe, the liquid supply box, the pump body, the inoculation pipe and the electromagnetic valve in the inoculation mechanism, the precise quantitative inoculation of the perforated mushroom stick body is realized, the effects of improving the inoculation efficiency and the inoculation accuracy and reducing the waste of the mushroom liquid are achieved, and due to the fact that the punching and the inoculation are completed in the same equipment, the labor intensity is reduced. Pollution possibly caused by transferring of the mushroom sticks among different devices is avoided, meanwhile, precise inoculation and early-stage punching operation supplement each other, and the remarkable advantages of the integrated device in the aspects of guaranteeing the product quality and improving the production efficiency are further embodied.

The invention discloses a rice wine brewing starter propagation raw material liquid bacterium inoculation device, and relates to the technical field of wine brewing. An electric heating wire is arranged in the tank body; a tank cover is mounted at the top of the tank body; a discharging pipe is mounted at the bottom of the tank body, and an electromagnetic valve I is mounted on the outer wall of the discharging pipe; a supporting strip is fixed on the outer wall of the tank body, and a grounding plate is fixed at the bottom of the supporting strip; an annular pipe is mounted on the inner wall of the tank body, and leakage holes are formed in the outer wall of the annular pipe; a mounting base is fixed to the outer wall of the tank body, and a first box body is fixed to the top of the mounting base. The temperature, humidity and carbon dioxide content in the tank body can be adjusted, so that the temperature, humidity and carbon dioxide content in the tank body are always located at proper numerical values; therefore, the inoculation efficiency and success rate of the koji in the tank body are improved, and the quality of wine is finally improved.

The invention belongs to the technical field of biological medicines, and particularly discloses an MEV-hBD3 mRNA vaccine for combined prevention of brucellosis and tuberculosis, the amino acid sequence of the vaccine comprises a plurality of CD8 + T cell epitopes, and the amino acid sequence of the CD8 + T cell epitopes at least comprises one of SEQ ID NO: 6 to SEQ ID NO: 18; the amino acid sequence of the vaccine further comprises a plurality of CD4 + T cell epitopes, and the amino acid sequence of the CD4 + T cell epitopes at least comprises one sequence from SEQ ID NO: 19 to SEQ ID NO: 32; the amino acid sequence of the vaccine further comprises a plurality of B cell epitopes, and the amino acid sequence of the B cell epitopes at least comprises one sequence of SEQ ID NO: 33 to SEQ ID NO: 41. The MEV-hBD3 mRNA vaccine provided by the invention has good immunogenicity, and is expected to provide an immunological strategy and a theoretical basis for emergency prevention and control of further prevalence of brucellosis and tuberculosis in the future.

The invention discloses an inoculator suitable for bag-grown edible fungi, comprising a frame and a conveying device, a mushroom stick queuing device, a disinfecting device, a punching device, an inoculating device, a film sticking device, a film pressing device, a circumferential positioning device, an air positive pressure device and a film pressing device installed on the frame. The mushroom stick queuing device, the disinfecting device, the punching device, the inoculating device, the film sticking device and the film pressing device are sequentially arranged above the conveying belt of the conveying device. The air positive pressure device is arranged above the punching device and the inoculating device, and its air inlet is connected with the punching station and the inoculating station. The circumferential positioning device comprises left and right positioning mechanisms and a front positioning mechanism. The front positioning mechanism is arranged above the conveying belt corresponding to the punching device and the inoculating device respectively. The left and right positioning mechanisms are arranged on both sides of the conveying belt corresponding to the front positioning mechanism. After the conveying belt conveys the mushroom sticks to the front positioning mechanism, the left and right positioning mechanisms are started and clamp the mushroom sticks to achieve consistency in the delivery of the mushroom sticks.

The automatic inoculation device is characterized in that a feeding hole is formed in the upper part of a drying box, a discharging hole is formed in the side wall of the drying box, a material receiving plate and a drying device are arranged in the drying box, and a material pushing device is arranged on one side, far away from the discharging hole, of the material receiving plate; the rolling fluidized bed is arranged on the side edge of the drying box, and the sampling device is arranged above the rolling fluidized bed; an inlet and an outlet are respectively formed in two sides of the oscillation device; the inlet is connected with the rolling fluidized bed; an inlet is formed in one side wall of the constant-temperature cultivation box and connected with an outlet of the oscillation device. The vinasse is dried by the drying device and sterilized at high temperature, the dried vinasse is pushed to the discharge port by the pushing device and falls onto the rolling fluidized bed from the discharge port, and the liquid strain is added into the vinasse on the rolling fluidized bed by the sampling device in the conveying process. And then the vinasse and the strains are uniformly shaken by the shaking device and then are conveyed into the constant-temperature cultivation box for cultivation, and the process does not depend on manpower, so that the microbial inoculum and the vinasse can be prevented from being polluted, and the inoculation efficiency is improved.