Application of serotype 5 haemophilus parasuis (HPs) vaccine strain
A technology of Haemophilus suis and Haemophilus suis disease, applied in the application field of serotype 5 Haemophilus parasuis strains, can solve the problems of low immune cross-protection, large difference in virulence and pathogenicity, and achieve Good protective effect, long duration, and stable biological characteristics
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Embodiment 1
[0015] Example 1: Isolation, purification and identification of serotype 5 Haemophilus parasuis XX0306 strain.
[0016] 1. Culture medium for production
[0017] Preparation of TSA solid medium: Accurately weigh 40g of TSA powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15min. 0.01% NAD of bacteria, mixed evenly and poured out for later use.
[0018] Preparation of TSB liquid medium: Accurately weigh 30g of TSB powder, dissolve in 940mL of distilled water, shake well, and then sterilize by high-pressure steam at 121°C for 15 minutes. That's it.
[0019] 2. Isolation, purification and identification of Haemophilus parasuis
[0020] 2.1 Separation and purification
[0021] In 2003, a large number of pigs suspected to be infected by Haemophilus parasuis appeared in a large-scale pig farm in Jiangsu, China. They were characterized by persistent fever, pleurisy, peritonitis, and arthritis. The lungs, pleural effus...
Embodiment 2
[0048] Example 2: Application of serotype 5 Haemophilus parasuis XX0306 strain in Haemophilus parasuis disease vaccine.
[0049] 1 strain
[0050] The strain used for seedling production is Haemophilus parasuis XX0306 strain of serotype 5, and its preservation number is CCTCC NO: M2013095. Strong virulence and good immunogenicity.
[0051] 2 Preparation of strains for production
[0052] 2.1 First-level production seed culture Take the lyophilized material of the basic seeds of Bacillus parasuis type 5, dissolve it, inoculate it on TSA / NAD solid medium, and cultivate it at 37°C for 24 hours. In TSB / NAD liquid medium, shake at 180r / min at 37°C for 18-24 hours. Then the culture is harvested, and after passing the pure inspection, it is used as the primary production seed and stored at 2-8°C for no more than 2 days.
[0053] 2.2 Propagation of secondary production seeds Take primary production seed cultures, inoculate TSB / NAD liquid medium at a volume ratio of 1:100, and cult...
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