Rice planthopper killing metarhizium anisopliae preparation and application thereof

A technology of Metarhizium anisopliae and rice planthopper, applied in application, insecticide, biocide, etc., can solve the problem of wet dispersion characteristics, storage stability, large impact on field control effect, poor wet dispersion effect, and short storage period of products and other problems, to achieve the effect of being suitable for large-scale production and large-scale promotion and use, outstanding control effect, and maintaining biological activity

Active Publication Date: 2015-05-13
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the activity of fungal spores is affected by wetting agents, the dosage of surfactants is often reduced in formulations, resulting in poor wetting and dispersing effects, while the product shelf life is still short, rarely exceeding 12 months
Due to the problem of wet dispersing characteristics, storage stability and the field control effect is greatly affected by ultraviolet radiation, although wettable powders for insecticidal fungi have been registered, they have not been widely used.

Method used

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  • Rice planthopper killing metarhizium anisopliae preparation and application thereof
  • Rice planthopper killing metarhizium anisopliae preparation and application thereof
  • Rice planthopper killing metarhizium anisopliae preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Virulence, sporulation and heat resistance of CQMa421 strain after inoculation with rice planthopper

[0026]Inoculate the CQMa421 strain on a 1 / 4 SDA plate, incubate at 28°C for 15 days, collect mature conidia, disperse them with 0.05% (W / V) Tween-80, and prepare a final concentration of 1×10 7 spores / ml suspension. The rice planthoppers reared indoors were transferred into 500ml triangular flasks with wheat seedlings, 30-50 rice planthoppers per bottle, sprayed and inoculated with a spray tower (POTTER, BURKARD company), 5 bottles were inoculated, each bottle was inoculated with 0.5 ml. After inoculation, it is placed in a bioassay room with a room temperature of 28°C and a humidity of 50-70%. The rice planthopper dead worms that were the first to die were selected, and placed in a 28°C incubator for 3-5 days of moisturizing culture to make fresh spores grow on the surface of the insects; the spores were collected to inoculate rice planthoppers 12-16 times...

Embodiment 2

[0033] Example 2 Protective effect of nano silicon on Metarhizium anisopliae

[0034] According to Table 2, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-silicon were first mixed, and then 45% diatomaceous earth, 5% CMC and different contents of O-30 and HEL-40 were added and mixed.

[0035] UV protection: disperse the preparation sample with water to make a concentration of 1×10 7 After spores / mL of spore suspension, take 100μl of spore suspension and spread it on 1 / 4 SDA plate, mix well with glass and put it in 290-310nm ultraviolet lamp (978mW / m 2 ) under vertical irradiation for 5 hours, and then cultured in a 28°C incubator for 24 hours to measure the germination rate (the method is the same as above), and repeat 3 times. The data were processed and analyzed with SPSS software.

[0036] Storage experiment: Store the preparation sample in a sealed container at 28°C for 2 months, and disperse it with water to make a concentration of 1×10 7 After spore...

Embodiment 3

[0041] Example 3 Preparation of wettable powder of Metarhizium anisopliae CQMa421 and its wettability, storage period and control effect.

[0042] According to Table 3, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-silicon were first mixed, and then diatomaceous earth, CMC, O-30 and HEL-40 with different contents were added.

[0043] Determination of wettability of wetting and dispersing agent: Take 100mL of standard water and pour it into a 250mL beaker. Place the beaker in a constant temperature water bath at 25°C±1°C, so that the liquid level is flush with the water bath level. When the hard water reaches 25°C±1°C, accurately weigh 5.0g of the sample to be tested and place it on a watch glass. Do not disturb the liquid level excessively. Immediately record the time with a stopwatch when adding the sample until the sample is completely wetted (the fine powder film remaining on the liquid surface can be ignored). Note down the wetting time (accurate to ...

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Abstract

The invention relates to application of a metarhizium anisopliae CQMa421 strain in preparation of a medicament for preventing and treating rice planthoppers. The medicament for preventing and treating the rice planthoppers is prepared from the following components by weight percent: 35-52% of metarhizium anisopliae CQMa421 dry spore powder, 40-50% of diatomite, 1-2% of nanometer silicon, 3-5% of sodium carboxymethylcellulose, 2-4% of O-30 and 2-4% of HEL-40. The rice planthoppers can be infected by the metarhizium anisopliae CQMa421 strain, and the fatality rate can reach 32.6 percent. The metarhizium anisopliae CQMa421 strain prepared by a toxicity increasing method has the fatality rate of 98.7-100%, LT50 is shortened by 57.4-61.4%, and the effect is very prominent. The components of the metarhizium anisopliae preparation are effectively matched to play a synergistic interaction role, and filed efficacy on the rice planthoppers can reach 80% above by spraying the metarhizium anisopliae preparation added with water. The preparation has good ultraviolet resistance and dispersing and wetting properties, the biological activity in long-term storage is kept, and the storage time is up to 12 months.

Description

technical field [0001] The invention relates to a new application of a strain of Metarhizium anisopliae, in particular to a preparation of Metarhizium anisopliae for killing rice planthoppers and an application thereof, belonging to the field of biological pesticides. technical background [0002] Rice planthopper is the main migratory pest of rice. It is widely distributed in the main rice areas of the country and seriously threatens my country's grain production. At present, broad-spectrum chemical pesticides are mainly used to control rice planthoppers, which not only directly affects human health and the safety of aquaculture industry, but also kills natural enemies of rice planthoppers and destroys the ecological balance of rice fields due to the development of rice planthopper resistance and the extensive use of chemical pesticides. , seriously weakening the natural control ability in the field, which will easily lead to the rampant and difficult control of rice planth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04C12R1/645
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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