5results about How to "Improve insecticidal efficiency" patented technology

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

This invention discloses a protein-based nanomaterial, its preparation method, and its application as a nucleic acid delivery carrier. The preparation method includes: modifying BSA with 1,6-hexanediamine as a modifier; dissolving the obtained BSA-NH2 and 2-iminothionyl pentane hydrochloride in PBS buffer to obtain a mixed solution; dissolving dodecyl acrylate and triethylamine in dimethyl sulfoxide and adding them to the mixed solution for stirring at room temperature; and dialysis purification. This invention grafts low-density dodecyl acrylate hydrophobic carbon chains onto the surface of cationic BSA to construct a bifunctional carrier with both charge-mediated encapsulation and transmembrane delivery capabilities. The hydrophobic chains mimic the hydrophobic environment of biological membranes, reducing the energy barrier and enhancing affinity to achieve efficient internalization, promoting the interaction between the carrier and the cell membrane; while amino modification endows it with pH-responsive dissociation ability, thereby enabling endosome escape.

The invention discloses application of thuringirin in prevention and treatment of cotton aphid, green peach aphid, armyworm and soybean cyst nematode, and belongs to the field of microbial pesticides. The invention discovers that the thurinin has the biological activity of killing cotton aphid, green peach aphid, mythimna separata and soybean cyst nematode for the first time, has a wide application prospect, and can be developed into a novel environment-friendly biological control agent.

The present application provides a protein and its use in improving the virulence of a parasporal crystal protein of Bacillus thuringiensis, which comprises a protein having an amino acid sequence shown in SEQ ID NO: 1. The protein of the present application can effectively improve the virulence of the parasporal crystal protein, especially the insecticidal capacity, after cooperating with the parasporal crystal protein, thereby reducing the use amount of the parasporal crystal protein, lowering the insecticidal cost, improving the insecticidal efficiency, and being suitable for large-scale popularization and application.

The application belongs to but is not limited to the technical field of Blattella germanica prevention and treatment, and discloses a Blattella germanica prevention and treatment method and system based on nanoparticle delivery of lethal gene dsRNA, which comprises the following steps: Blattella germanica lethal gene full-length cloning; plasmid construction and dsRNA expression and purification; and RNAi feeding. The application first confirms an efficient RNAi lethal target point for Blattella germanica, ensures the efficiency of the technical scheme, realizes low-cost and large-scale preparation of an active ingredient of an RNAi insecticide, breaks through the cost bottleneck of commercial application, creatively constructs a nanocomposite delivery system, efficiently overcomes the technical bottleneck of oral delivery of RNAi, and provides an environment-friendly practical prevention and treatment scheme, and has important application value and prospect.