Metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid

A technology for Metarhizium anisopliae and tea inchworm, which is applied to Metarhizium anisopliae CHMA-005 and its application field in the control of tea inchworm, can solve problems such as differences in pathogenicity of target pests, and achieves high spore germination rate and spore production. Large, easy to cultivate effects

Active Publication Date: 2020-09-15
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although some biocontrol strains are used to control pests, due to the host specificity of strains from populations and host sources, the pathogenicity of target pests is also different, and it is necessary to explore some relatively broad-spectrum insect pests. Fungal Strains for Controlling Tea Geometrids

Method used

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  • Metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid
  • Metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid
  • Metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid

Examples

Experimental program
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Embodiment 1

[0018] Example 1 Isolation and identification of Metarhizium anisopliae CHMA-005 and its spore production and spore germination rate determination

[0019] (1) Isolation of strains: The tea geometrid larvae that died of natural disease were collected from the humus soil of Beifeng tea garden in Fuzhou, Fujian. In the ultra-clean workbench, use a sterile scalpel to scrape the spore powder on the surface of the insect body into 10 mL of sterile water, filter the spore stock solution through degreasing gauze, and then take 100 μL of the spore stock solution and spread it evenly on the PDA plate medium, and then Place them in a light incubator with a temperature of (28±1)°C, a relative humidity of (90±5)%, and a photoperiod of L:D=12:12 for 10 days.

[0020] (2) Morphological identification: After the isolated strains were cultured for 10 days, first observe the characteristics of the colony and conidia, and then observe the morphological characteristics of the strains, conidia an...

Embodiment 2

[0030] Embodiment 2 Scarab anisopliae (Metarhizium anisopliae) CHMA-005 is to the infection lethal effect of tea geometrid (Ectropisobliqua hypulina Wehrli) larvae

[0031] Get (Example 1) the isolated bacterial strain and inoculate it on the PDA medium plate, place the HGZ-150 type of temperature (28 ± 1) ℃, relative humidity (90 ± 5)%, photoperiod L:D=12:12 Cultivate in a light incubator for 10 days, then add 5 mL of sterile water containing 0.05% Tween-80 to delyze the spores, and filter through three layers of degreasing gauze to obtain the spore stock solution; use a Neubauer blood cell counting board to count the spores under a NIKONE200 biological microscope The number of spores in the stock solution and the spore concentration were calculated, and then the spore stock solution was diluted to 1.00×10 with sterile water. 4 , 1.00×10 5 , 1.00×10 6 , 1.00×10 7 and 1.00×10 8 5 concentrations such as cfu / mL, 1500 times of 10% lambda-cyhalothrin water emulsion was used as...

Embodiment 3

[0035] The preparation method of the biological bacterial agent taking scarab metarhizium anisopliae (Metarhizium anisopliae) CHMA-005 as active ingredient, the steps are as follows:

[0036] (1) Inoculate the isolated and purified Metarhizium anisopliae CHMA-005 bacterial strain on a PDA plate, and cultivate it at 28° C. for 8 days;

[0037] (2) Collect the mature spores cultivated on the PDA plate, disperse with 0.05% (0.05g / 100ml) Tween-80 aqueous solution;

[0038] (3) Prepared to a final concentration of 1 × 10 8 For spore suspension in cfu / ml, 10-12 sterilized sponge blocks (4-6 cm in length, 2-4 cm in width, and 0.3-0.5 cm in height) are immersed in the spore suspension in a room where the space has been previously sterilized After fully absorbing the solution, put the sponge block into a sterile culture dish (diameter 9cm), place it in a constant temperature incubator, and incubate at 28°C for 6 days;

[0039] (4) After the surface of the sponge block soaked in the s...

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Abstract

The invention discloses metarhizium anisopliae CHMA-005 and application thereof in prevention and control of tea geometrid, and belongs to the technical field of biological prevention and control. Themetarhizium anisopliae CHMA-005 disclosed by invention is simple to culture, rapid in growth on culture medium, large in sporulation quantity and high in spore germination rate, and infection lethaleffect on tea geometrid larvae can reach 80% or above, the metarhizium anisopliae CHMA-005 is relatively safe to natural enemies, capable of replacing part of chemical pesticides of tea geometrid, capable of reducing the dosage of the chemical pesticides and beneficial to maintaining ecological balance.

Description

technical field [0001] The invention relates to the technical field of biological control, and more specifically relates to a strain of metarhizium anisopliae CHMA-005 and its application in preventing and controlling tea geometrids. Background technique [0002] Tea geometrid (Ectropis obliqua hypulina Wehrli), commonly known as arch worm, measuring inchworm, hanging silkworm, belongs to Lepidoptera (Lepidoptera) geometridae (Geometridae); it is one of the most common and most serious pests in tea gardens. Tea geometrids reproduce quickly, spread rapidly, and are prone to outbreaks. Young larvae bite the edges of young leaves and cause reticular translucent film spots, and older larvae bite leaves and cause nicks; when serious, the new shoots and old leaves of tea trees are eaten Bald, leaving only bare branches, directly affects the yield, quality and economic value of tea. At present, the prevention and control of this pest focuses on the use of chemical pesticides, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30C12R2001/645C12N1/145
Inventor 陈峰胡进锋陈文乐王俊王长方吴玮
Owner INST OF PLANT PROTECTION FAAS
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