Application of Metarhizium anisopliae strain in preparation of insecticide for vegetable pest insects

A technology for Metarhizium anisopliae and Metarhizium anisopliae, which is applied in the directions of pesticides, applications, biocides, etc., can solve the problems of wet dispersion characteristics, storage stability, large influence of field control effect, poor wet dispersion effect, and no large-scale application, etc. problem, to achieve good anti-UV and wetting and dispersing characteristics, outstanding effect, and maintain the effect of biological activity

Inactive Publication Date: 2015-05-13
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the activity of fungal spores is affected by wetting agents, the dosage of surfactants is often reduced in formulations, resulting in poor wetting and dispersing effects, while the product shelf life is still short, rarely exceeding 12...

Method used

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  • Application of Metarhizium anisopliae strain in preparation of insecticide for vegetable pest insects
  • Application of Metarhizium anisopliae strain in preparation of insecticide for vegetable pest insects
  • Application of Metarhizium anisopliae strain in preparation of insecticide for vegetable pest insects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Strain CQMa421 and its virulence to Plutella xylostella and Plutella xylostella after being inoculated with the virulence of Plutella xylostella and Plutella xylostella

[0026] The CQMa421 strain was inoculated on a 1 / 4 SDA plate, cultured at 28°C for 15 days, mature conidia were collected, dispersed with 0.05% (W / V) Tween-80, and prepared to a final concentration of 1×10 7 spores / ml suspension. Transfer the indoor-raised flea beetle adults into 500ml triangular flasks with cabbage leaves, 30-50 per bottle, spray inoculation with a spray tower (POTTER, BURKARD company), inoculate 5 bottles, and inoculate 0.5 ml in each bottle . After inoculation, in the raw side room at room temperature of 28°C and humidity of 50-70%; select the flea beetle that is the first to be infected and die, and put it in a 28°C incubator for 3-5 days to make it grow on the surface of the insect. fresh spores. The spores were collected and reconstituted to a final concentration of...

Embodiment 2

[0033] Example 2 Protective effect of nano aluminum hydroxide on Metarhizium anisopliae

[0034] According to Table 2, the pure spore powder of Metarhizium anisopliae CQMa421 was first mixed with nano-aluminum hydroxide, and then 40% diatomaceous earth, 5% CMC and different contents of Tween-80 and OP-10 were added and mixed.

[0035] UV protection: disperse the preparation sample with water to make a concentration of 1×10 7 After spores / mL of spore suspension, take 100μl of spore suspension and spread it on 1 / 4 SDA plate, mix well with glass and put it in 290-310nm ultraviolet lamp (978mW / m 2 ) under vertical irradiation for 5 hours, and then cultured in a 28°C incubator for 24 hours to measure the germination rate (the method is the same as above), and repeat 3 times. The data were processed and analyzed with SPSS software.

[0036] Storage experiment: Store the preparation sample in a sealed container at 28°C for 2 months, and disperse it with water to make a concentrat...

Embodiment 3

[0040] Example 3 Preparation of wettable powder of Metarhizium anisopliae CQMa421 and its wettability, storage period and control effect.

[0041] According to Table 2, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-aluminum hydroxide were first mixed, and then diatomaceous earth, CMC, Tween-80 and OP-10 with different contents were added.

[0042] Determination of wettability of wetting and dispersing agent: Take 100mL of standard water and pour it into a 250mL beaker. Place the beaker in a constant temperature water bath at 25°C±1°C, so that the liquid level is flush with the water bath level. When the hard water reaches 25°C±1°C, accurately weigh 5.0g of the sample to be tested and place it on a watch glass. Do not disturb the liquid level excessively. Immediately record the time with a stopwatch when adding the sample until the sample is completely wetted (the fine powder film remaining on the liquid surface can be ignored). Note down the wetting time...

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Abstract

The invention relates to an application of a Metarhizium anisopliae CQMa421 strain in preparation of insecticide for preventing and controlling Striped flea beetle or plutella xylostella. The strain is capable of infecting Striped flea beetles and plutella xylostella with fatality rates being 60.9% and 70.8% respectively. The Metarhizium anisopliae CQMa421 strain with enhanced toxicity, which is prepared according to a method disclosed by the invention, has a fatality rate reaching 100% and achieves an outstanding prevention and control effect. The insecticide for preventing and controlling Striped flea beetles or plutella xylostella comprises dry conidial powder of Metarhizium anisopliae CQMA421 (44-60%), kieselguhr (30-40%), nano-aluminum trihydrate (0.5-1%), sodium carboxymethylcellulose (3-5%), tween-80 (3-5%) and OP-10 (3-5%). All the components in the Metarhizium anisopliae insecticide are matched effectively to take a synergistic effect. After being mixed with water, the Metarhizium anisopliae insecticide is applied in a spraying way to achieve a field prevention effect of 80% or above for rice planthopper. The Metarhizium anisopliae insecticide has excellent wetting dispersion characteristic, wherein the wetting time is less than 60s; and in addition, the bioactivity during long-term storage can be retained and the storage time is as long as 12 months.

Description

technical field [0001] The invention relates to a new application of a strain of Metarhizium anisopliae, in particular to a preparation and application of Metarhizium anisopliae for killing vegetable pests, and belongs to the field of biological pesticides. Background technique [0002] Flea beetle, which belongs to Coleoptera and Chrysophyteidae pests, referred to as flea beetle, is an important pest of leafy vegetables. Plutella xylostella, commonly known as small caterpillars, also mainly damage leafy vegetables. These two pests often occur simultaneously or alternately in vegetable fields, bringing huge losses to vegetable production every year. At present, chemical pesticides are mainly used to control the flea beetle and diamondback moth, which seriously affect human health and destroy the ecological environment. Therefore, the use of safe microbial agents to replace chemical pesticides has become an important development direction for the control of flea beetle and ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/14
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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