Metarhizium rileyi and application thereof

A technology of Metarhizium anisopliae and Reysteria, applied in the field of microorganisms, can solve the problems of polluted environment, strong pathogenicity of Spodoptera frugiperda larvae, and easy to produce drug resistance, etc., to achieve high spore germination rate, strong pathogenicity, and not easy The effect of drug resistance

Active Publication Date: 2020-09-15
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Metarhizium anisopliae described in the present invention is not only highly pathogenic to Spodoptera frugiperda larvae, but also has an ovicidal effect, can effectively control the population of Spodoptera

Method used

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  • Metarhizium rileyi and application thereof
  • Metarhizium rileyi and application thereof
  • Metarhizium rileyi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation and identification of FJMR1 strain

[0020] 1.1 Isolation and purification of strains and determination of pathogenicity

[0021] The diseased Spodoptera frugiperda criss-cross adults collected from Zhangzhou and Sanming corn fields in Fujian were placed in 5 mL sterile EP tubes and brought back to the laboratory, and lightly cultured on PDA medium (potato 200 g, glucose 20 g, agar 20 g, distilled water 1000 mL). Lightly shaking zombified zombophiles, at least some conidia on the surface of the body fell on the PDA medium, cultured in an incubator at 26°C, 16L / 8D alternating light and dark, and 75% humidity, and transferred the conidia to new cells after sporulation. Purification was performed on PDA medium. The purified strains were stored in a 13°C incubator with rye slant medium, and the pathogenicity of the strains was determined by dipping and inoculating with spore suspension. The test insects began to die 6-7 days after inoculation, and the surface of...

Embodiment 2

[0029] Lethal effect of Metarhizium anisopliae FJMR1 on the larvae of Spodoptera frugiperda

[0030] 2.1 Insects to be tested

[0031] The indoor population of Spodoptera frugiperda was collected from a cornfield near Yunxiao County, Zhangzhou City, Fujian Province, and the larvae were raised on artificial diet until pupation. After the adults emerged, they mated and laid eggs, and the second-instar larvae of the next generation were taken as the test insects. The feeding conditions of Spodoptera frugiperda were 75% humidity, 26°C temperature, and photoperiod L:D=14:10.

[0032] 2.2 Preparation of FJMR1 spore suspension

[0033] The isolated bacterial strain FJMR1 was inoculated onto SMAY medium (maltose 40g / L, peptone 10g / L, yeast extract 10g / L, agar 1.8%), placed in a light incubator at 26°C for cultivation (16L / 8D, light and dark Alternate), after it produces a large number of conidia, wash out the conidia with sterile water and filter with four layers of gauze, take a d...

Embodiment 3

[0042] Effects of Metarhizium anisopliae FJMR1 on egg hatching rate and newly hatched larvae of Spodoptera frugiperda

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Abstract

The invention relates to Metarhizium rileyi and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Metarhizium rileyi FJMR1 strain disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 18552. The Metarhizium rileyi not only has strong pathogenicity on Spodoptera frugiperda larvae, but also has an ovicidal effect, can effectively control the population quantity of Spodoptera frugiperda, and can solve the technical problems that in the prior art, chemical pesticides are adopted for preventing and treating Spodoptera frugiperda in corn to pollute the environment and easily generate drug resistance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Metarhizium anisopliae and its application. Background technique [0002] Spodoptera frugiperda (J.E.Smith) belongs to Lepidoptera Noctuidae and can damage more than 100 important crops such as corn, rice and soybean. At present, it is difficult to control Spodoptera frugiperda. Under the pressure of long-term pesticide selection, it has developed resistance to a variety of pesticides including organophosphates, carbamates, and pyrethroids. It is imperative to seek safe, efficient and sustainable measures to control fall armyworm. Insecticidal fungi have many types, are easy to spread, are environmentally safe and are not easy to produce drug resistance. Therefore, the use of insecticidal fungi to control Spodoptera frugiperda has become an important development direction. [0003] Metarhizium rileyi (Metarhizium rileyi) is a widely distributed entomopathogen...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12R2001/645C12N1/145
Inventor 丁雪玲何玉仙杨秀娟郑宇姚凤銮
Owner INST OF PLANT PROTECTION FAAS
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