30results about How to "Strong insecticidal toxicity" patented technology

The invention relates to insecticidal protein, a coding gene of the insecticidal protein and a purpose of the insecticidal protein. The insecticidal protein comprises (a) protein consisting of amino acid sequences shown by SEQ ID NO. 2 or 1st to 640th bits; or (b) protein which has the insecticidal activity and is derived from (a) through substituting, deleting or adding one or several amino acids from / into the amino acid sequences of (a); or (c) protein generated through expression of the nucleic acid molecule containing the 1st to 1920th bit nucleotide sequences of SEQ ID NO. 1; or (d) protein generated through expression of the nucleic acid molecule containing complementary sequences hybridized with the 1st to 1920th bit nucleotide sequences of SEQ ID NO. 1 under the strict conditions; or (e) protein generated through nucleic acid molecule expression with similar codes to the nucleotide sequences of (d). The insecticidal protein has high expression quantity and strong toxicity on insect pests.

The invention relates to a pest control constructor and a method thereof. The constructor includes Cry2Aa protein and Cry1A protein. The pest control constructor and the method disclosed by the invention control pests through the Cry2Aa protein and the Cry1Ab / Ac protein generated in plants; the constructor and the method, compared to an agricultural control method, a chemical control method and a biological control method in the prior art, are not only strong in toxicity and thorough in effect, but also capable of preventing and controlling pests through protecting the whole plants in the whole growth period; and the constructor is free of pollution and residues, stable in effect, simple, convenient and economical.

The invention relates to insecticidal protein, a coding gene of the insecticidal protein and a purpose of the insecticidal protein. The insecticidal protein comprises (a) protein consisting of amino acid sequences shown by SEQ ID NO. 2; or (b) protein which has the insecticidal activity and is derived from (a) through substituting, deleting or adding one or several amino acids from / into the amino acid sequences of (a); or (c) protein generated through expression of the nucleic acid molecule containing the nucleotide sequences of SEQ ID NO. 1; or (d) protein generated through expression of the nucleic acid molecule containing nucleotide sequences, wherein the nucleotide sequences have complementary sequences hybridized with the nucleotide sequences of SEQ ID NO. 1 under the strict conditions; or (e) protein generated through expression of the nucleic acid molecule containing nucleotide sequences, wherein the nucleotide sequences have the similar codes to the nucleotide sequences of (d). The insecticidal protein has high expression quantity and strong toxicity on insect pests.

The invention relates to an insecticidal protein, and an encoding gene and use thereof. The insecticidal protein comprises (a) protein formed by an amino acid sequence shown in SEQ ID NO:2, or (b) protein which has insecticidal activity and is derived from (a) in a manner of substituting and / or deleting and / or adding one or more amino acids to the amino acid sequence in (a). The insecticidal protein disclosed by the invention is especially applicable to expression in monocotyledons, especially rice, so that not only are the expression quantity and the stability of PIC10-01 insecticidal protein significantly improved, but also the toxicity of the PIC10-01 insecticidal protein on insects and injurious insects, especially lepidoptera insect pests is significantly enhanced.

The invention provides an insecticidal protein, as well as a coding gene and application thereof. The insecticidal protein comprises (a) protein containing amino acid sequence shown in SEQ ID NO:2; or (b) protein which is an amino acid sequence in (a) substituted, lost or added by one or more amino acids and is derived from (a) with insecticide activity; or (c) protein which is produced through expression of nucleic acid molecules containing a nucleotide sequence shown in SEQ ID NO:1; or (d) protein produced through expression of nucleic acid molecules containing the nucleotide sequence which has a complementary sequence hybridized with the nucleotide sequence shown in SEQ ID NO:1 in a strict condition; or (e) protein produced through expression of nucleic acid molecules containing the nucleotide sequence which has the same codes with the nucleotide sequence of (d). The insecticidal protein has the advantages of high expression capacity and strong toxicity to insects and pests.

The invention relates to an insecticidal protein, and a coding gene and use thereof. The insecticide protein comprises (a) a protein formed by an amino acid sequence shown as SEQ ID NO:2; or (b) a protein derived from (a), wherein the protein is substituted and / or deleted and / or added with one or more amino acids in the amino acid sequence in (a) and has the insecticidal activity; or (c) a protein formed by the amino acid sequence which has at least 90% of sequence homology with the SEQ ID NO:2. The insecticide protein provided by the invention is not only high in expression quantity, but also good in stability, and is strong in toxicity to injurious insects.

The invention relates to an insecticidal protein, and coding genes and purposes thereof. The insecticidal protein comprises: (a) a protein composed of an amino acid sequence showed by SEQ ID: 2 or the 1-640 amino acid residues of the sequence; (b) proteins that derive from (a), which are formed by substitution, deletion or addition of one or more amino acids of the amino acid sequence of (a) and have insecticidal activity; (c) proteins expressed by nucleic acid molecules that comprise the 1-1920 nucleotide sequence of SEQ ID NO: 1; (d) proteins expressed by nucleic acid molecules that comprise the complementary sequence which hybridizes with the 1-1920 nucleotide sequence of SEQ ID NO: 1 under strict condition; or (e) proteins expressed by nucleic acid molecules that comprises similar codes with the nucleotide sequence of (d). The insecticidal protein of the invention is high in expression, and has strong toxicity for insect pests.

The invention relates to a construct for controlling insect pests and a method thereof. The construct comprises a Cry1Ca protein and a Cry1A protein. According to the construct for controlling insect pests and the method thereof, the protein Cry1Ca and Cry1Ab / Ac are generated in plants so as to control the insect pests. Compared with agricultural control methods, chemical control methods and biological control methods are used in the prior art, the method is high in toxicity, complete in effect, pollution-free, residue-free, stable in effect, simple, convenient and economic, and the plants are subjected to whole-growth period and whole-plant protection so as to prevent intrusion of the insect pests.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence from 1st to 650th sites shown as SEQ ID NO:2, or a protein (b) formed by the amino acid sequence shown as the SEQ ID NO:2, or a protein (c) with insecticidal activity, which is derived from the protein (a) or (b), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a) or (b), or a protein (d) formed by an amino acid sequence with at least 90% of sequence identity with the amino acid sequence of the protein (a) or (b). The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention discloses a green muscardine fungus conidium microcapsule and a preparation method and application thereof. The green muscardine fungus conidium microcapsule disclosed by the invention comprises a core material and a wall material and is prepared by adopting complex coacervation, wherein the core material comprises green muscardine fungus conidium powder and simethicone; the wall material comprises A-type gelatin and Arabic gum; the microcapsule is regularly shaped, and the particle size is about 35 to 50 microns. According to the microcapsule disclosed by the invention, the ultraviolet resistance of green muscardine fungus is obviously improved, the storage time of conidium is prolonged, and the insecticidal toxicity for red imported fire ants is also relatively high; the defect that the field application stress resistance of green muscardine fungus conidium is low is overcome, the control effect for agricultural and forest pests is obviously improved, and the microcapsule has important market applications and popularization values.

The invention discloses a medicament for preventing and treating main pests on a pineapple and a use method thereof. Aiming at the main pests including mealy bugs, giant crickets and mole crickets on the pineapple, a pesticide granule or powder is prepared by taking thiamethoxam, sulphur and amino acid as main raw materials and absorbing by utilizing a filler; an aqueous solution of the pesticide granule or powder is poured in soil in which the pineapple is cultivated or the granule is directly spread on the surface of the soil in which the pineapple is cultivated, so that the main pests including the mealy bugs, the giant crickets and the mole crickets on the pineapple are effectively prevented and treated; a pesticide formula is simple, convenient to use and suitable for pest prevention and treatment in the pineapple cultivating process.

The invention relates to a pest control constructor and a method thereof. The constructor includes Cry2Aa protein and Cry1A protein. The pest control constructor and the method disclosed by the invention control pests through the Cry2Aa protein and the Cry1Ab / Ac protein generated in plants; the constructor and the method, compared to an agricultural control method, a chemical control method and a biological control method in the prior art, are not only strong in toxicity and thorough in effect, but also capable of preventing and controlling pests through protecting the whole plants in the whole growth period; and the constructor is free of pollution and residues, stable in effect, simple, convenient and economical.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence shown as SEQ ID NO:2, or a protein (b) with insecticidal activity, which is derived from the protein (a), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a), or a protein (c) formed by an amino acid sequence with at least 90% of sequence identity with the SEQ ID NO:2. The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention discloses a strain of metarhizium anisopliae and its application, belonging to the field of biotechnology. The strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 21, 2021, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, preservation number: CGMCC No. 3.19644, and the taxonomic name is : Shijiazhuang Metarhizium shijiazhuangense. The insecticidal test shows that the strain has a good killing effect on leather ticks and blood ticks, especially the effect on blood ticks is more significant. The strain can effectively control the populations of leather ticks and blood ticks and reduce the harm caused by them. It is a biocontrol strain with potential significance in the control of leather ticks and blood ticks. It has insecticidal properties Strong toxicity, good insecticidal effect and so on.

The invention relates to metarhizum anisopliae for preventing and controlling palm pest opisina arenosella walker and application of the metarhizum anisopliae. The strain is named as metarhizum anisopliae LSY-1 and is preserved in China Center for Type Culture Collection on November 9, 2015 with preservation number of CCTCC M 2015672. The metarhizum anisopliae LSY-1 provided by the invention is applicable to the prevention and control of the opisina arenosella walker; a test of direct smearing administration shows that the metarhizum anisopliae LSY-1 has a good insecticidal action on the palm pest opisina arenosella walker and the metarhizum anisopliae can be used for effectively controlling the population quantity of the opisina arenosella walker; and the metarhizum anisopliae, as a bio-control strain which has a potential significance to prevent and control the opisina arenosella walker, can offer help for the development of the palm industry in China.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence from 1st to 650th sites shown as SEQ ID NO:2, or a protein (b) formed by the amino acid sequence shown as the SEQ ID NO:2, or a protein (c) with insecticidal activity, which is derived from the protein (a) or (b), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a) or (b), or a protein (d) formed by an amino acid sequence with at least 90% of sequence identity with the amino acid sequence of the protein (a) or (b). The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention relates to an insecticidal protein, and an encoding gene and use thereof. The insecticidal protein comprises (a) protein formed by an amino acid sequence shown in SEQ ID NO:2, or (b) protein which has insecticidal activity and is derived from (a) in a manner of substituting and / or deleting and / or adding one or more amino acids to the amino acid sequence in (a). The insecticidal protein disclosed by the invention is especially applicable to expression in monocotyledons, especially rice, so that not only are the expression quantity and the stability of PIC10-01 insecticidal protein significantly improved, but also the toxicity of the PIC10-01 insecticidal protein on insects and injurious insects, especially lepidoptera insect pests is significantly enhanced.

The invention provides an insecticidal protein, as well as a coding gene and application thereof. The insecticidal protein comprises (a) protein containing amino acid sequence shown in SEQ ID NO:2; or (b) protein which is an amino acid sequence in (a) substituted, lost or added by one or more amino acids and is derived from (a) with insecticide activity; or (c) protein which is produced through expression of nucleic acid molecules containing a nucleotide sequence shown in SEQ ID NO:1; or (d) protein produced through expression of nucleic acid molecules containing the nucleotide sequence which has a complementary sequence hybridized with the nucleotide sequence shown in SEQ ID NO:1 in a strict condition; or (e) protein produced through expression of nucleic acid molecules containing the nucleotide sequence which has the same codes with the nucleotide sequence of (d). The insecticidal protein has the advantages of high expression capacity and strong toxicity to insects and pests.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence shown as SEQ ID NO:2, or a protein (b) with insecticidal activity, which is derived from the protein (a), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a), or a protein (c) formed by an amino acid sequence with at least 90% of sequence identity with the SEQ ID NO:2. The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence shown as SEQ ID NO:2, or a protein (b) with insecticidal activity, which is derived from the protein (a), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a), or a protein (c) formed by an amino acid sequence with at least 90% of sequence identity with the SEQ ID NO:2. The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention discloses bacillus thuringiensis for inhibiting stem bleeding disease of coconut and killing lepidoptera pests and application thereof. The bacillus thuringiensis has a collection number of CGMCC No.7967. A bacillus thuringiensis strain WENCHANG-BT20 is directly separated and screened from a soil sample on the coast of Hainan and carries four insecticidal crystal protein encoding genes of crylAa, crylBe, cryllia and cry2Aa. The bacillus thuringiensis has high insecticidal capacity and good insecticidal effect and has a certain effect of inhibiting fungal diseases.

The invention discloses a green muscardine fungus conidium microcapsule and a preparation method and application thereof. The green muscardine fungus conidium microcapsule disclosed by the invention comprises a core material and a wall material and is prepared by adopting complex coacervation, wherein the core material comprises green muscardine fungus conidium powder and simethicone; the wall material comprises A-type gelatin and Arabic gum; the microcapsule is regularly shaped, and the particle size is about 35 to 50 microns. According to the microcapsule disclosed by the invention, the ultraviolet resistance of green muscardine fungus is obviously improved, the storage time of conidium is prolonged, and the insecticidal toxicity for red imported fire ants is also relatively high; the defect that the field application stress resistance of green muscardine fungus conidium is low is overcome, the control effect for agricultural and forest pests is obviously improved, and the microcapsule has important market applications and popularization values.

The invention relates to an insecticidal protein and a coding gene and an application thereof. The insecticidal protein contains a protein (a) formed by an amino acid sequence from 1st to 650th sites shown as SEQ ID NO:2, or a protein (b) formed by the amino acid sequence shown as the SEQ ID NO:2, or a protein (c) with insecticidal activity, which is derived from the protein (a) or (b), and is formed by replacing and / or deleting and / or adding one or more amino acid in the amino acid sequence of the protein (a) or (b), or a protein (d) formed by an amino acid sequence with at least 90% of sequence identity with the amino acid sequence of the protein (a) or (b). The insecticidal protein has the advantages of high expression quantity, good stability, and high toxicity to insect pests.

The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2 or a sequence of amino acid residuses at positions 1 to 640 thereof; or (b) a protein derived from (a) by substitution, deletion, or addition of one or more amino acid residuses of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a sequence of amino acid residuses at the positions 1 to 1920 of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with the sequence of nucleotides at the positions 1 to 1920 of SEQ ID NO:1 under stringent conditions. The protein has high expression level and strong toxicity against pests.

The invention relates to insecticidal protein, a coding gene of the insecticidal protein and a purpose of the insecticidal protein. The insecticidal protein comprises (a) protein consisting of amino acid sequences shown by SEQ ID NO. 2 or 1st to 640th bits; or (b) protein which has the insecticidal activity and is derived from (a) through substituting, deleting or adding one or several amino acids from / into the amino acid sequences of (a); or (c) protein generated through expression of the nucleic acid molecule containing the 1st to 1920th bit nucleotide sequences of SEQ ID NO. 1; or (d) protein generated through expression of the nucleic acid molecule containing complementary sequences hybridized with the 1st to 1920th bit nucleotide sequences of SEQ ID NO. 1 under the strict conditions; or (e) protein generated through nucleic acid molecule expression with similar codes to the nucleotide sequences of (d). The insecticidal protein has high expression quantity and strong toxicity on insect pests.

The invention relates to an insecticidal protein, and a coding gene and use thereof. The insecticide protein comprises (a) a protein formed by an amino acid sequence shown as SEQ ID NO:2; or (b) a protein derived from (a), wherein the protein is substituted and / or deleted and / or added with one or more amino acids in the amino acid sequence in (a) and has the insecticidal activity; or (c) a protein formed by the amino acid sequence which has at least 90% of sequence homology with the SEQ ID NO:2. The insecticide protein provided by the invention is not only high in expression quantity, but also good in stability, and is strong in toxicity to injurious insects.

The present invention relates to the field of biological control, and particularly provides a biological source insecticide and a preparation method thereof. The biological source insecticide comprises an active component having insecticidal or insect-resistant activity on pests, and the biological source insecticide can prevent the pests from generating drug resistance, the insecticide provided by the invention has the advantages of broad spectrum, high efficiency, no killing of beneficial organisms, no harmful residue, no pollution, no toxic or side effect and the like.