Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof

A technology of red palm weevil and metarhizium anisopliae, applied in the field of microorganisms, to achieve the effects of strong insecticidal virulence, loss reduction, and population control

Inactive Publication Date: 2014-11-26
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no relevant report on the use of fungi to control the red palm weevil

Method used

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  • Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof
  • Metarrhizum anisopliae for preventing and treating palm insect rhynchophorus ferrugineus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Screening and Identification of Example 1 Bacterial Strains

[0015] 1. Screening of bacterial strains

[0016] Take the collected red palm weevil beetle sample, first disinfect it with 70% alcohol for one minute, wash it with sterile water, then use 0.1% mercuric chloride to disinfect the surface of the susceptible sample for 30 seconds, and wash it three times with sterile water. Pick a small amount of zombie tissue and inoculate it in the separation SDAY medium (4% glucose, 1% peptone, 1% yeast powder, 2% agar and 500μg / mL streptomycin), and place it upside down at 28±1°C for 3-4 days , select single spores to inoculate in the purified SDAY medium (4% glucose, 1% peptone, 1% yeast powder and 2% agar), 3 points per dish, arranged in a triangle, and obtain insecticidal strains after single spore purification , named Metarhizum anisopliae (Metarhizum anisopliae) WENCHANG-LV2, has been preserved in the General Microbiology Center of China Committee for the Collection of...

Embodiment 2

[0024] Example 2 Determination of Indoor Insecticidal Activity of the Bacterial Strain to the Red Palm Weevil

[0025] Take the isolated and purified Metarhizum anisopliae (Metarhizum anisopliae) WENCHANG-LV2 strain, put the conidia powder into a 10ml sterile centrifuge tube, add 5mL of 0.1% Tween 80 sterile water, and vortex The shaker was shaken for 15 minutes to mix well, and then the number of spores in each milliliter suspension was measured with a hemocytometer, and the strain was configured so that the spore concentration was 1.0×10 8 per mL, and stored in a refrigerator at 4°C for future use. The spores of Metarhizum anisopliae (Metarhizum anisopliae) WENCHANG-LV2 strain were prepared into 5 concentrations of oil preparations by gradient dilution method, the concentrations were 1.0×10 8 , 5×10 7 , 2.5×10 7 , 1.25×10 7 , 6.25×10 6 pieces / ml. The preparation method of the spore oil suspension is: scrape the conidia powder of the strain, add the mixed oil agent with...

Embodiment 3

[0028] Embodiment three bacterial strains outdoor control effect

[0029] Bring the oil agent of Metarhizum anisopliae (Metarhizum anisopliae) WENCHANG-LV2 strain to the field, dilute with mixed oil to contain conidia 1×10 8 / mL, and stir evenly, spray in ultra-low volume.

[0030] Spray control began on May 6, 2013. At that time, the weather was fine, the temperature was 34°C, the wind was breezy, and the crosswind included angle was 50° when spraying. The spray droplets distributed more and evenly on the tree head (near the growth point), and the coverage density was average. 23.8 drops / cm 3 . Check once a week, select 3 coconut trees with the same damage shape and dissect each time, and count the number of live insects and infected dead insects. The results are shown in Table 2. It can be seen from the results that after 15 days of treatment, the survival rate of larvae in the trunk began to decline significantly, and the survival rate was only 41.25% after 40 days, indi...

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Abstract

The invention relates to metarrhizum anisopliae for preventing and controlling palm insect rhynchophorus ferrugineus and application thereof. The strain is named metarrhizum anisopliae WENCHANG-LV2 is preserved in the China General Microbiological Culture Collection Center on June 9th, 2013 with the preservation number of CGMCC NO.7692. The metarrhizum anisopliae WENCHANG-LV2 provided by the invention can be applied to rhynchophorus ferrugineus prevention and control. Direct application testing indicates that the metarrhizum anisopliae WENCHANG-LV2 has a good insecticide effect, can be used for effectively controlling the number of species of rhynchophorus ferrugineus, is a biocontrol strain which has a potential meaning in preventing and treating rhynchophorus ferrugineus, and favors the development of palm industry in China.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a Metarhizium anisopliae strain and its application, in particular to a Metarhizium anisopliae strain for preventing and treating the palm pest, Ruthia anisopliae, and its application. Background technique [0002] Red Palm Weevil (Rhynchophorus ferrugineus Oliv.) belongs to Coleoptera (Coleoptera) Weevil family (Curculionidae) Palm Weevil genus Rhynchophorus sp., the English name is Red Palm Weevil (RPW for short), also known as Asian palm weevil, rusty palm Elephant, coconut beetle, cryptorhynchus, Indian red palm weevil, etc. The red palm weevil is mainly distributed in Taiwan, Fujian, Hong Kong, Hainan, Guangdong, Guangxi, Shanghai, Yunnan, Tibet (Medog) and other places in China. The results of the pest risk analysis of the red palm weevil show that it is a high-risk pest and is easy to colonize in many areas of China. It poses a great threat to my country's oil palm, areca nut,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 孙晓东覃伟权刘丽钟宝珠李朝绪张晶
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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