A strain of Metarhizium anisopliae and its application

A technology of metarhizium anisopliae and strains, applied in the direction of application, fungi, biocides, etc., can solve the problems of tick death, long duration, etc., achieve good insecticidal effect, good killing effect, and reduce harm

Active Publication Date: 2022-06-28
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Metarhizium anisopliae germinates into ticks with spores, reproduces rapidly and produces toxins, resulting in the death of ticks. After the spores of the bacteria in the worms are released, they can infect other healthy ticks, and repeated infection in the population will cause a large number of ticks to die. drug lasts long

Method used

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  • A strain of Metarhizium anisopliae and its application
  • A strain of Metarhizium anisopliae and its application
  • A strain of Metarhizium anisopliae and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0021] This example provides the isolation and morphological identification of Metarhizium anisopliae SH3-4.

[0022] 1. Isolation of Metarhizium anisopliae

[0023] The soil samples collected in Xibaipo, Shijiazhuang City, Hebei Province, were brought back to the laboratory and treated with sterile ddH 2 After the O was moistened, 200g of soil was transferred to 500mL polyethylene plastic cups, 10 Tenebrio molitors were placed in each plastic cup, and each cup was covered with a ventilated lid with 2-2.5mm oxygen holes. The motility of Tenebrio molitor increases the chance of contact with fungi in the soil, and the survival of Tenebrio molitor is monitored weekly. Take out the larvae of Metarhizium anisopliae (such as figure 1 shown), respectively with sterile ddH 2 After washing, placed in a sterile petri dish (35 × 10 mm) covered with moist filter paper, using sterile toothpicks to isolate the fungus from each infected larvae, and transferred to containing 0.6 g of strep...

Embodiment 2

[0027] The larvae of the model insect Tenebrio molitor were infected with 9 strains of bacteria, and the experimental results were as follows Figure 4 As shown, choose the SH3-4 bacterial strain with high insecticidal activity to carry out insecticidal activity assay to D. forest tick and Haemophilus longhorn, and the steps are as follows:

[0028] The SH3-4 strain was inoculated on a PDAY plate with 1% yeast, and placed in a constant temperature and humidity incubator (25°C, relative humidity 90%) for 15 days in the dark. Transfer the fungal spores to a 0.01% Tween-20 solution with a sterilized wooden spatula, filter with sterile gauze to remove large pieces of mycelium to obtain a fungal conidia suspension, and then use a hemocytometer to determine the final spore concentration, The spore germination rate of Metarhizium anisopliae SH3-4 in Shijiazhuang was 95%. 1 mL of the diluted spore solution (2×10 8 spores / mL) were sprayed on the body surface of Haematoma longhorne (9...

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Abstract

The invention discloses a strain of metarhizium anisopliae and its application, belonging to the field of biotechnology. The strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 21, 2021, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, preservation number: CGMCC No. 3.19644, and the taxonomic name is : Shijiazhuang Metarhizium shijiazhuangense. The insecticidal test shows that the strain has a good killing effect on leather ticks and blood ticks, especially the effect on blood ticks is more significant. The strain can effectively control the populations of leather ticks and blood ticks and reduce the harm caused by them. It is a biocontrol strain with potential significance in the control of leather ticks and blood ticks. It has insecticidal properties Strong toxicity, good insecticidal effect and so on.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a strain of Metarhizium anisopliae and application thereof. Background technique [0002] Ticks belong to the Arthropoda Arachnida subclass Acarina and the order Ticks. They are obligate blood-sucking ectoparasites that spread a variety of pathogens and cause diseases. big hazard. Ticks are the vector arthropods that transmit the most types of pathogens. Tick-borne diseases mainly include Lyme disease, tick-borne spotted fever, Q fever and fever with thrombocytopenia syndrome. The harm caused by health is second only to mosquitoes, and how to control it is an urgent problem to be solved. [0003] At present, the main methods for controlling ticks are: (1) Chemical pesticides and insecticides. Chlorpyrifos, malathion, amithiophos, and hexaflumuron are all commonly used pesticides, but they are prone to drug resistance, and the mixed use has a good control effect (patent number: CN ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 于志军刘敬泽阿伯尔法兹尔·马苏迪王参王敏吴昌昊
Owner HEBEI NORMAL UNIV
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