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Metarhizium GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof

A technology of Metarhizium anisopliae and antibiotics, applied in the field of microorganisms, can solve problems such as drug resistance

Inactive Publication Date: 2010-10-13
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether it is medical antibiotics or agricultural antibiotics, there is currently an unavoidable problem, that is, how to obtain high-efficiency, broad-spectrum, low-toxicity, and safe antibiotics
For medical antibiotics, there is a more serious resistance problem

Method used

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  • Metarhizium GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof
  • Metarhizium GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof
  • Metarhizium GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: the acquisition of Metarhizium anisopliae bacterial strain of the present invention

[0055] A species of Cordyceps was collected from the Yangai tea farm in Guiyang City, Guizhou Province, placed in a sterile test tube, and brought back to the laboratory for isolation and cultivation in the following two ways.

[0056] Method 1: According to the tissue separation method (Fang Zhongda, Plant Pathogen Research Method (3rd Edition). Beijing: China Agricultural Press, 1998), the collected Cordyceps sinensis was washed with water to clean the surface mud, dried in the shade, and respectively used sterile knives Cut off the sclerotium and fruiting bodies, pick small pieces of tissue with a sterile inoculation needle, sterilize with 70% alcohol or 0.1% mercury solution, wash 2-3 times with sterile distilled water, and place it on a PDA medium plate, 25-28 Cultivate at ℃ for 2-4 days, pick a single colony, and obtain a pure culture strain. Store on Sabouraud med...

Embodiment 2

[0058] Embodiment 2: Preparation of GYYA0601 strain fermentation product

[0059] After the activation of each strain, pick about 5-10mm 2 The size of the hyphae block to the 100mL seed culture solution (Sabouraud medium: glucose 40g, peptone 10g, KH 2 PO 4 1g, MgSO 4 0.5g, distilled water 1L) in a 250mL shake flask, cultured at 26-28°C for 3-4 days, with a rotation speed of 150-180r / min. After the fermentation was terminated, the cultivated seed culture was inoculated into 200mL fermentation medium (the fermentation medium was improved Sabouraud's medium: glucose 25g, soybean powder 5g, yeast extract 2g, KH 2 PO 4 1g, MgSO 4 0.5g, distilled water 1L) in a 500mL shake flask, cultured at 26-28°C for 7-9 days, with a rotation speed of 150-180r / min, after the fermentation was terminated, the fermentation product was obtained, and the fermentation product had broad-spectrum antibiotic activity.

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Abstract

The invention discloses a Metarhizium taii GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof. The preservation number of the Metarhizium taii GYYA0601 is CGMCC No.2880. The Metarhizium taii GYYA0601 strain is used for producing the antibiotics by fermentation; a fermentation method comprises the steps of strain activation, primary seed preparation and fermentation; and the obtained fermentation product has broad-spectrum antibiotic activity.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Metarhizium anisopliae capable of producing broad-spectrum antibiotics and its application. Background technique [0002] In the long history of biological evolution, animals, plants, and microorganisms have formed metabolites with diverse structures and functions in order to adapt to or resist adverse external environments. The diversity of microbial metabolites and the diversity of biological activities are unmatched by general synthetic compounds. Almost all drug screening models can screen physiologically active substances from microorganisms. Since the discovery and development of penicillin in the last century, humans have discovered a large number of medical or agricultural drugs from microbial metabolites. But the microorganisms that have been studied and detected only account for about 3% of the microorganisms. In addition, among natural drug sources...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02C12R1/645
Inventor 肖建辉钟建江肖代敏
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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