131results about How to "Growth inhibitory effect" patented technology

The invention relates to a separation and purification method of endophytic fungi from ginkgo biloba. The separation and purification method comprises the following operation steps of: (1) preparing ginkgo biloba tissues: collecting roots, stems and leaves of the ginkgo biloba and preserving under the condition that the temperature is 4 DEG C; (2) performing surface disinfection and sterile detection on the ginkgo biloba tissues; and (3) performing separation culture on the endophytic fungi from the ginkgo biloba tissues. The endophytic fungi from the ginkgo biloba, described by the invention, is named as Fusariumsolani T-7 and currently collected in China General Microbiological Culture Collection Center designated by State Intellectual Property Office, the collection data is July 20, 2011, and the collection number is CGMCC No. 5089. The endophytic fungi from the ginkgo biloba, disclosed by the invention has the microbiology position of the Fusariumsolani T-7 with broad-spectrum antimicrobial activity, and the strain is simultaneously found to have an obvious effect of inhibiting the growth of mycelia against pathogenic fungi of six tested plants. According to the separation and purification method disclosed by the invention, resources of the plant endophytic fungi are utilized to get a novel agricultural antibiotic type natural component for resisting diseases caused by the plant pathogenic fungi.

The invention provides a humanized modified anti-CD147 chimeric antibody HcHAb18 and an application thereof. The anti-CD147 human-rat chimeric antibody is prepared on the basis of obtaining hybridoma cells of an anti-human CD147 monoclonal antibody and antibody HcHAb18 genes of the hybridoma cells, wherein the variable region of light chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:1; the variable region of heavy chains of the anti-CD147 human-rat chimeric antibody has the amino acid sequence shown in SEQ ID NO:2; fucose are not combined in N-acetylglucosamine in a reducing end of a carbohydrate chain at a Fc segment of the anti-CD147 monoclonal antibody in a specific host cell; the glycoform of the anti-CD147 monoclonal antibody is of a mannose type (MAN 5); the anti-CD147 chimeric antibody has antibody dependent cell-mediated cytotoxicity (ADCC). The invention further provides an expression vector and an engineering cell strain of the anti-CD147 chimeric antibody and the application of the anti-CD147 chimeric antibody taken as a cancer treating medicine.

The invention relates to a biological antibacterial patch for bedsore surface. The composite film patch mainly comprises four layers, namely a bottom breathable base (1) coated with adhesive, a second layer made of nonwoven rayon (2), a third layer made of biological antibacterial dressing (3), and a surface paper isolation layer (4), wherein one end of the bottom breathable base is provided with an easy tear edge (5). The biological antibacterial dressing is made from chitosan, acetic acid, lysozyme, gelatin, clorhexidine acetate, aerosil, and water. The biological bacterial dressing including no any additive has the functions of fine antibiosis, inflammation diminishing, hemostasis, promoting cell growth, promoting wound healing and the like, and has the advantages of high water content, fine water retention, high toughness and good breathability. The biological antibacterial patch for bedsore surface is mainly used for treating bedsores, burns and surgical infections.

Belonging to the technical field of biological pesticides, the invention provides a Gentiana nubigena endophyte applicable to biological control of plant diseases and its fermentation process. The strain involved in the invention is Bacillus pumilus LD-b1, which is preserved in the General microbiological center of China Committee for Culture Collection of Microorganisms on May 14, 2012, and has a preservation number of CGMCC No.6112. Mixed strain agar plate punching method experiments show that the strain LD-b1 can inhibit the growth of a plurality of plant disease fungi. LD-b1 can substantially inhibit Valsa mali from eroding apple fruits. In greenhouse pot experiments, the LD-b1 can achieve a cucumber Mycosphaerella arachidicola control effect of 81.2%. The fermentation process consists of: culturing LD-b1 in a seed tank for 24h to a logarithmic growth phase, inoculating 5% of the LD-b1 into a production tank according to an inoculation amount, controlling the initial pH at 7.5 and the fermentation temperature at 30DEG C, maintaining dissolved oxygen over 5%, and performing fed-batch fermentation for 68h, thus obtaining a bacterial amount over 10<10>cfu/mL. With the advantages of fast growth and wide antibacterial spectrum, the strain LD-b1 has good application prospects in biological control of plant diseases.

The invention discloses a naphthazarin nucleus oxialkyl and acyl substituted alkannin derivative, its preparation and an application thereof. The activity of the compound provided by the invention is equivalent to or better than that of a corresponding alkannin nucleus oxialkyl and substituted derivative. By the use of the derivative, cytotoxicity of some compounds is reduced or disappears, and the animal body shows a certain effect of inhibiting tumor growth through an antitumor test. The derivative can be used as an antitumor drug or prodrug to treat malignant tumor. Simultaneously, the preparation method provided by the invention has advantages of easily-obtained raw materials, short synthesis route and high optical purity.

The present invention discloses a biological fresh-keeping method of armeniaca vulgaris lam, and belongs to the technical field of fruit fresh-keeping. According to the present invention, with the treatments such as spraying and soaking, the respiration and the water loss of the armeniaca vulgaris lam fruit can be inhibited, the microorganism growth on the armeniaca vulgaris lam fruit surface can be inhibited, the fresh-keeping period of the armeniaca vulgaris lam can be effectively prolonged, the fresh-keeping effect on the armeniaca vulgaris lam is good, the method is green and safe, and the obtained armeniaca vulgaris lam can be directly eaten at any time after being stored.

The invention discloses preparation and application of nanoparticle doped RNA hydrogel for targeted triple negative breast cancer. The RNA hydrogel is a pure RNA system formed in a rolling loop transcription mode, a large number of GC bonds are generated in the transcription process, a large number of sites are provided for introduction of DOX, a large number of RNA replica structures generated through rolling loop replication are polyanion aggregate, in view of strong electronegativity of the polyanion aggregate, electropositive MnO2@Ce6 nanoparticles are introduced, and the polyanion hydrogel can stabilize the colloidal positive ion MnO2@Ce6 particles, and thus enters breast cancer cells in a targeting mode for collaborative treatment. The nanoparticle doped RNA hydrogel can be used fora drug sustained release system, is good in biocompatibility, and has wide prospects in the fields such as growth inhibition effects of targeted cells, namely MDA-MB-231 tumor cells and inhibition ofcancer metastasis and recurrence.

The invention relates to a fusion gene which comprises a GnRH gene sequence, a p53 gene sequence and an optional gene sequence of an oxygen-dependent protein degradation structural domain (ODD) of a hypoxia-inducible factor between the GnRH gene sequence and the p53 gene sequence. The invention also relates to recombinant fusion proteins, an expression recombinant of the fusion genes, engineeringbacteria obtained from the expression recombinant and pharmaceutical applications of the expression recombinant and the fusion proteins. A function of resisting tumor of p53 can be efficiently improved according to the invention.

The invention belongs to the field of medical examination, and relates to a semen momordicae extractive as well as a preparation method and anti-tumour use thereof. The semen momordicae extractive having anti-tumour activity is prepared from corresponding components separated and purified by taking high performance liquid chromatography as the method after a sample to be measured and extracted is quenched. Found from in-vitro pharmacological experiments, the semen momordicae extractive is capable of obviously inhibiting proliferation of cancer cells by inducing cell apoptosis and retarding cell growth cycle, has an obvious inhibiting effect on human gastric carcinoma cells and other tumour cells, and can be used as the active component for preparing medicines for treating solid tumours, particularly medicines for treating human gastric carcinoma, human prostatic cancer or colon cancer. The preparation method disclosed by the invention is simple, convenient, low in cost and high in yield; and the extractive is high in component purity and good in reproducibility.

The invention provides a micro-nano tissue engineering scaffold with an immune treatment function and a preparation method thereof. The scaffold with the immune treatment function is prepared by surface activation modification and grafting of the surface of an electrostatic spun fiber scaffold with an antibody. The preparation method comprises the following steps: (1) preparation of the electrostatic spun fiber scaffold; (2) surface activation modification of the electrostatic spun fiber scaffold; (3) grafting with the antibody. Through grafting of the surface of the electrostatic spun scaffold with the antibody, on one hand, apoptosis of tumor cells is directly induced by antibody release, at the same time, antibody release can induce activation of dendritic cells in a tumor microenvironment, specific immune response is stimulated, cytokines are released and killing T cells are activated to kill tumor, and the tumor cell growth effect is indirectly inhibited; on the other hand, the scaffold after the antibody is released provides a solid carrier for tissue regeneration, and induces regenerated tissue regeneration. Finally, double functions of the biological scaffold in immune regulation inhibition of the tumor and promotion of regeneration of new tissues are achieved.

The invention discloses a high-grade composite nourishing flour and noodle, which is characterized by the following: adopting walnut pulp, soy and wheat as raw material, inhibiting leukemia, colon cancer, lung carcinoma and gastric cancer.

The invention relates to a traditional Chinese medicine composition for treating nausea and vomiting caused by chemotherapy, traditional Chinese medicine decoction-free granules and application and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition is prepared from the following raw materials: 6-15 parts of ginger processed pinellia, 10-15parts of dried ginger, 6-15 parts of exocarpium citri grandis, 10-15 parts of white poria, 3-12 parts of rice water processed fructus aurantii, 10-15 parts of pogostemon cablin, 2-4 parts of preparedrhizoma coptidis with rhizoma zingiberis recens juice, 3-5 parts of liquor processed scutellaria baicalensis, 15-30 parts of reed rhizome, 5-12 parts of bamboo shavings, 5-9 parts of perilla leaves, 9-15 parts of codonopsis pilosula Nnannf and 2-6 parts of raw licorice roots. The traditional Chinese medicine decoction-free granules are prepared from the above-mentioned single traditional Chinese medicine formula granules by weight. The traditional Chinese medicine composition and the traditional Chinese medicine decoction-free granules are applied to preparation of drugs for treating nausea and vomiting caused by chemotherapy. The traditional Chinese medicine composition has the advantages of replenishing qi, strengthening spleen, invigorating the stomach, calming the adverse-rising energy, eliminating phlegm, regulating body immunity and inhibiting tumor growth; synergizing with chemotherapeutic drugs to achieve enhanced sensitivity, alleviating vomiting symptoms; and regulating digestive tract functions.

The invention relates to a preparation method of a pyrolysis oil urea-formaldehyde resin organic covering mat, and belongs to the technical field of landscaping. According to the preparation method, landscaping waste is taken as raw materials, landscaping waste pyrolyzate pyrolysis oil is used for preparing pyrolysis oil urea-formaldehyde resin, and the organic covering mat is prepared by gluing the pyrolysis oil urea-formaldehyde resin and the crushed landscaping waste, so that the shifting and loss problems of a traditional organic mat due to blowing and raining are effectively solved, and the life cycle and the environmental protection effect of the organic covering mat are ensured. Furthermore, the organic covering mat can serve as an effective slow release nitrogenous fertilizer, andthe promoting effect on plant growth is improved; phenols substances are added to the pyrolysis oil urea-formaldehyde resin due to the pyrolysis oil, so that the effect of inhibiting weed seeds from germinating and growing is improved; and the organic covering mat has better mildewproof and antibacterial effects, and the aging resistance of the organic covering mat is improved. The organic covering mat can improve the supply of soil nutrients and inhibit weeds, and is long in life cycle and obvious in economic benefit.

The present invention relates to an euphorbiaceae plant active substance for restraining growth of cancer and/or tumor and application thereof. The euphorbiaceae plant active substance is extracted from the euphorbiaceae plant by alcohol and furthermore can be extracted by n-hexane. The invention further relates to use of the euphorbiaceae plant active substance in preparing a composition for restraining growth of the cancer and/or tumor cell.

The invention discloses a traditional Chinese medicine composition for resisting a liver cancer and a lung cancer. The traditional Chinese medicine composition is prepared from a rhizoma paridis total saponin extract, a curcuma extract and a dioscorease rhizome extract of which the mass ratio is 1:(10-20):(1-10). An in-vitro MTT process activity test proves that the traditional Chinese medicine composition disclosed by the invention has a growth inhibition effect on an LA795 lung cancer cell line and an HepG2 hepatoma cell line; IC50 respectively can be up to below 20mg/mL (dose). An in-vivo experiment proves that the traditional Chinese medicine composition disclosed by the invention has a significant growth inhibition effect on mice T739 lung cancer metastasis in mice, an HepA liver cancer and an H22 lung cancer, and the tumor control rates respectively can be up to 53%, 55% and 57%. Compared with component administration of components forming the composition, the tumor control effect of the composition disclosed by the invention has a synergistic enhancement effect.

The invention discloses a method for inhibiting expansion of anaerobic active sludge, comprising the following steps of: firstly adding an inorganic salting liquid containing 5-10% of ferrous sulphate, 10-20% of cupric nitrate, 5-20% of cobalt nitrate and the like into wastewater, stirring, adding active substances such as 5-20% of kaolin, 10-20% of calcium carbonate and the like into water after 20-40 min, adjusting pH value of water to 6.5-8.5, and carrying out oxygen-enriched aeration at normal temperature for 2-4 hours. According to the invention, with the addition of the inorganic heavy metallic salts and active substances, the growth of anaerobic bacteria is inhibited and consolidation performance of sludge is raised so as to improve sludge settleability; and in addition, through oxygen-enriched aeration, production of anaerobic bacteria can be further inhibited so as to prevent expansion of anaerobic active sludge.

The invention relates to a drug composition with the effect of anti-lung cancer and an application in preparing anti-lung cancer drug; the raw materials of the drug are as follows by weight portions: 10 to 30 of common selfheal fruit-spike, 10 to 50 of spreading hedyotis herb, 10 to 50 of hairyvein agrimonia herb and bud, 10 to 30 of yerbadetajo herb and 6 to 20 of dogwood. The drug extract is prepared by the following method which comprises the steps of: (1) extracting with 75 percent to 95 percent of alcohol for 1 to 4 times with 1 to 3 hours for each time; (2) carrying out extraction to the drug dreg extracted by the step (1) with 50 percent to 75 percent of alcohol for 1 to 4 times with 1 to 3 hours for each time; (3) combining the extracts of the (1) and (2), recovering the alcohol, reducing pressure and concentrating to obtain macroporous absorption resin column on the concentrated solution, using 40 percent to 60 percent of alcohol for elution to obtain extract I and using 70 percent to 90 percent of alcohol for elution to obtain extract II; and (4) mixing the extract I and the extract II to obtain active components. The application of the drug composition in preparing the drug for anti-lung cancer has stronger capacity to inhibit the proliferative activity of human lung cancer cell SPC-A-1.

The invention discloses a Metarhizium taii GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof. The preservation number of the Metarhizium taii GYYA0601 is CGMCC No.2880. The Metarhizium taii GYYA0601 strain is used for producing the antibiotics by fermentation; a fermentation method comprises the steps of strain activation, primary seed preparation and fermentation; and the obtained fermentation product has broad-spectrum antibiotic activity.