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Semen momordicae extractive as well as preparation method and use thereof

A Momordicae semen extract and Momordicae semen technology are applied in the field of medical testing, can solve problems such as anti-tumor activity that have not yet been seen, and achieve the effects of high component purity, high reproducibility and high purity

Inactive Publication Date: 2013-06-26
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the unique anti-tumor activity of the extract of the fruit of the fruit of the tree, which has been proved by in vitro pharmacological experiments.

Method used

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  • Semen momordicae extractive as well as preparation method and use thereof
  • Semen momordicae extractive as well as preparation method and use thereof
  • Semen momordicae extractive as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of the fruit extract of the fruit

[0034] Grind the Chinese herbal medicines of the fruit of the tree to obtain the powder of the fruit of the tree, take 100g of the powder of the fruit of the tree, and extract it with 500ml of sodium acetate solution (20mM, pH 5.0) in the refrigerator at 4°C overnight, and freeze-dry the filtrate after centrifugation to obtain the extract (F), preserved in -20 ℃ refrigerator for subsequent use, after dissolving the lyophilized powder (F) of the fruit extract obtained by extraction in water, centrifugal filtration to remove a small amount of insoluble impurities, further separation by chromatography (chromatogram as shown in Fig. figure 1 shown), collect the 26-40min fractions, freeze-dry the combined collections and store them in a -20°C refrigerator for later use.

[0035] The chromatographic conditions are as follows:

[0036] Chromatographic column: YMC-Pack ODS-A (150×10mm, 5μm)

[0037] Column temperature...

Embodiment 2

[0045] Example 2. In vitro anti-tumor effect test of the fruit of the fruit

[0046] The SGC7901 cells in the logarithmic growth phase were inoculated in 96-well plates, and divided into blank control group, carboplatin control group and Saccharina seed extract treatment group (0.1, 0.2, 0.4, 0.6, 0.8, 1.0mg / ml), After culturing at 37°C for 72 hours, MTT assay was performed to calculate the inhibition rate of cell proliferation. The results showed that, with the increase of dose, the extract of Saccharina chinensis significantly inhibited the proliferation of SGC7901 cells (such as figure 2 shown).

Embodiment 3

[0047] Example 3. IC of different tumor cells of the fruit extracts F and F3 of the fruit 50 value

[0048] Human gastric cancer cells SGC7901 in logarithmic growth phase, human prostate cancer cells PC-3 and mouse colon cancer cells CT-26 were inoculated in 96-well plates respectively, and divided into blank control group, carboplatin control group and Saccharomyces japonicus extract In the treatment group (0.1, 0.2, 0.4, 0.6, 0.8, 1.0mg / ml), after cultured at 37°C for 48h, 72h, and 96h, the MTT test was carried out, and the inhibition rate of cell proliferation was calculated. The results showed that, with the dose increasing, the extract of Saccharomyces japonicus significantly inhibited the proliferation of various cancer cells (as shown in Table 2).

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Abstract

The invention belongs to the field of medical examination, and relates to a semen momordicae extractive as well as a preparation method and anti-tumour use thereof. The semen momordicae extractive having anti-tumour activity is prepared from corresponding components separated and purified by taking high performance liquid chromatography as the method after a sample to be measured and extracted is quenched. Found from in-vitro pharmacological experiments, the semen momordicae extractive is capable of obviously inhibiting proliferation of cancer cells by inducing cell apoptosis and retarding cell growth cycle, has an obvious inhibiting effect on human gastric carcinoma cells and other tumour cells, and can be used as the active component for preparing medicines for treating solid tumours, particularly medicines for treating human gastric carcinoma, human prostatic cancer or colon cancer. The preparation method disclosed by the invention is simple, convenient, low in cost and high in yield; and the extractive is high in component purity and good in reproducibility.

Description

technical field [0001] The invention belongs to the field of medical examination, and relates to active substances in traditional Chinese medicines, in particular to an extract of the fruit of a tree tree, a preparation method thereof, and an antitumor application. Background technique [0002] According to statistical reports, malignant tumors have become a difficult disease that seriously threatens human life, and are the second killer after heart disease. At present, chemotherapy is the first choice for the drug treatment of malignant tumors commonly used in clinical practice. However, chemotherapy will bring extremely serious side effects. Prior art research reports that some Chinese herbal medicines have unique features in the treatment of difficult and miscellaneous diseases, malignant tumors and the like. Therefore, finding and screening anti-tumor active ingredients from natural resources to further develop effective and low-toxic drugs for treating malignant tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/42A61P35/00
Inventor 郁韵秋刘洪瑞林志燕
Owner FUDAN UNIV
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