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Separation and purification method of endophytic fungi of solidago canadensis

An endophytic fungus, separation and purification technology, applied in the field of microorganisms, can solve the problems of long residual effect period, high toxicity, blind and indiscriminate application of pesticides, etc.

Inactive Publication Date: 2012-09-26
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safety issues. , human health and living environment issues

Method used

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  • Separation and purification method of endophytic fungi of solidago canadensis
  • Separation and purification method of endophytic fungi of solidago canadensis
  • Separation and purification method of endophytic fungi of solidago canadensis

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Embodiment

[0055] The endophytic fungus of Solidago canadensis described in the present invention is named as Trichophyllum yt03 ( Chaetomium globosum yt03), deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the date of deposit is July 20, 2011, and the deposit number is CGMCC No.5088;

[0056] The specific separation and purification steps of endophytic fungi from Solidago canadensis are as follows:

[0057] (1). Collect the stems and leaves of Solidago canadensis;

[0058] (2). Solid culture and sterility test of stems and leaves: Soak the stems collected in step (1) in 70% alcohol for 50 seconds and 0.2% sodium hypochlorite solution for 90 seconds; Soak in 70% alcohol for 40s, soak in 0.2% sodium hypochlorite solution for 60s; rinse the soaked stems and leaves with sterile water 3 times, and dry them in the shade to obtain sterilized stems and leaves; ...

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Abstract

The invention relates to a separation and purification method of endophytic fungi of solidago canadensis. The method comprises the following steps of: 1, collecting stems and leaves of the solidago canadensis; 2, carrying out solid culture and sterility test on the stems and the leaves; and 3, carrying out isolated culture on the endophytic fungi of tissues of the solidago canadensis to obtain the endophytic fungi of the solidago canadensis. A fungus colony has silk velvet texture and a villous surface, does not generate pigment and is light olivaceous and black brown at the back; and the gene complex of the endophytic fungi ITS of the solidago canadensis is composed of 602 basic groups (bp). The geomicrobiology status of a bacterial strain of chaetomiumglobosumyt03 with broad-spectrum antibacterial activity is determined, and the endophytic fungi is found to have relatively remarkable role in inhibiting mycelial growth of six provided plant pathogenic fungi through antibacterial test of the fermentation liquid of the chaetomiumglobosumyt03.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for separating and purifying microorganisms whose fermented products have the activity of inhibiting plant pathogenic fungi, that is, a method for separating and purifying endophytic fungi from Solidago canadensis. Background technique [0002] The main means of controlling crop diseases, insect pests and weeds in the process of agricultural production is the control of chemical pesticides, which play a positive role in reducing the damage of diseases, insect pests and weeds and ensuring high grain yields. However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 郭正彦花日茂柏钰操海群吴祥为李学德唐俊
Owner ANHUI AGRICULTURAL UNIVERSITY
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