Separation and purification method of endophytic fungi of solidago canadensis
An endophytic fungus, separation and purification technology, applied in the field of microorganisms, can solve the problems of long residual effect period, high toxicity, blind and indiscriminate application of pesticides, etc.
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[0055] The endophytic fungus of Solidago canadensis described in the present invention is named as Trichophyllum yt03 ( Chaetomium globosum yt03), deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the date of deposit is July 20, 2011, and the deposit number is CGMCC No.5088;
[0056] The specific separation and purification steps of endophytic fungi from Solidago canadensis are as follows:
[0057] (1). Collect the stems and leaves of Solidago canadensis;
[0058] (2). Solid culture and sterility test of stems and leaves: Soak the stems collected in step (1) in 70% alcohol for 50 seconds and 0.2% sodium hypochlorite solution for 90 seconds; Soak in 70% alcohol for 40s, soak in 0.2% sodium hypochlorite solution for 60s; rinse the soaked stems and leaves with sterile water 3 times, and dry them in the shade to obtain sterilized stems and leaves; ...
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