Composition for inhibiting tumor cells

A tumor cell and composition technology, applied in the field of plant extracts, can solve problems such as poor effect and inability to be widely used

Active Publication Date: 2017-05-17
树王台湾桧木精油实业坊 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In addition, although these plants have anti-oxidation and anti-inflammation functions, it is still unknown which diseases can be treated by these plants alone, and the extracts of these plants are not effective in inhibiting tumors, so they cannot be widely used , this is the two major disadvantages

Method used

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  • Composition for inhibiting tumor cells
  • Composition for inhibiting tumor cells
  • Composition for inhibiting tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] In this example, the composition for inhibiting tumor cells was firstly diluted in different times, then added to the breast cancer cell line (MCF-7) for culture, and then the MTT colorimetric method was used to carry out the inhibition of the growth of the breast cancer cell line by the composition Experiment to observe whether the composition has an inhibitory effect on the growth of breast cancer cell lines, and what the inhibitory concentration is.

[0042]First, inoculate breast cancer cell lines with good growth conditions into 96 microwell flat plates, with about 3,000 cells per well, and place them in 5% CO 2 , Cultivate in a 37°C incubator until overnight, add the composition for inhibiting tumor cells the next day, filter and sterilize with a 0.22 μm filter membrane before adding the composition, and then perform 2-fold serial dilution with sterile water, and take the diluted After vigorous shaking, the composition was added to 96 microwells containing cells, ...

Embodiment 2

[0048] In this example, the tumor cell inhibitory composition was firstly diluted in different times, then added to the lung fibroblast cell line (MRC-5) for culture, and then the MTT colorimetric method was used to carry out the effect of the composition on the lung fibroblast cell line Growth inhibition test, to observe whether the composition has the effect of inhibiting the growth of lung fibroblasts, and what the inhibitory concentration is.

[0049] First, inoculate the lung fibroblast cell line in good growth condition into a 96-microwell plate with about 3,000 cells per well, and place in 5% CO 2 , Cultivate in a 37°C incubator until overnight, add the composition for inhibiting tumor cells the next day, filter and sterilize with a 0.22 μm filter membrane before adding the composition, and then perform 2-fold serial dilution with sterile water, and take the diluted After vigorous shaking, the composition was added to 96 microwells containing cells, the final concentrat...

Embodiment 3

[0056] In this example, the composition for inhibiting tumor cells was firstly diluted in different multiples, and then added to the liver cancer cell line (HepG2) for culture, and then the MTT colorimetric method was used to conduct the growth inhibition test of the composition on the liver cancer cell line. To observe whether the composition has the effect of inhibiting the growth of liver cancer cell lines, and what the inhibitory concentration is.

[0057] First, inoculate the liver cancer cell lines in good growth condition into 96 microwell plates with about 3,000 cells per well, and place them in 5% CO 2 , Cultivate in a 37°C incubator until overnight, add the composition for inhibiting tumor cells the next day, filter and sterilize with a 0.22 μm filter membrane before adding the composition, and then perform 2-fold serial dilution with sterile water, and take the diluted After vigorous shaking, the composition was added to 96 microwells containing cells, the final con...

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PUM

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Abstract

The invention provides a composition utilizing natural materials. The composition comprises extracts of cypress wood, Cinnamomum kanehirae, Calocedrus formosana, Cinnamomum camphora, citronella, lindera glauca, cassia bark, Lengxiangshan, tea tree, Eucalyptus, Cryptomeria fortunei and acacia rachii, and the extracts are mixed in a specific ratio to form the tumor cell inhibition composition having an inhibition effect on tumor cell growth.

Description

technical field [0001] The invention relates to a plant extract obtained from plant raw materials, and a composition prepared according to a certain ratio. The composition has the function of inhibiting the growth of tumor cells. Background technique [0002] In the past 10 years, malignant tumors have ranked first among the top 10 causes of death, among which the mortality rates of lung cancer, liver cancer, and colorectal cancer are the top 3 for both men and women, and the mortality rates for women are ranked 4th and 5th respectively by breast cancer and pancreatic cancer , while men suffer from oral cancer and esophageal cancer. Both domestic and foreign doctors and researchers are committed to studying how to avoid or reduce the production or proliferation of tumor cells. [0003] Pulmonary fibrosis means that the lung tissue is affected by inflammation, causing the alveoli to be replaced by fibrous substances, making the originally soft lung tissue hard and lose its el...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61P35/00
Inventor 张美龄
Owner 树王台湾桧木精油实业坊
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