Culture medium for cultivating high-toxicity Metarhizium anisopliae and preparation of Metarhizium anisopliae and application of preparation

A technology of Metarhizium anisopliae and culture medium, which is applied in the field of plant protection, can solve the problems that the virulence of Metarhizium anisopliae cannot be effectively improved, and achieve the effect of improving the control effect, increasing the virulence, and increasing the infection rate

Active Publication Date: 2020-06-05
PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0009] Aiming at the problem that the prior art cannot effectively improve the virulence of Metarhizium anisopliae and be used to control Spodoptera frugiperda, the present invention provides a culture

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[0060] The present invention will be further described below in conjunction with specific embodiments.

[0061] Materials and Methods

[0062] 1. Materials

[0063] Metarhizium anisopliae NG-Ma040 (Metarhizium anisopliae): isolated from the soil of this laboratory from Spodoptera frugiperda, and preserved; the preservation number of Metarhizium anisopliae is CGMCC NO.19273, January 17, 2020 It is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing.

[0064] Freeze-dried powder of Spodoptera frugiperda: It is obtained from laboratory-raised 6th instar larvae after ultra-low temperature freeze-drying, stirring and crushing.

[0065] Liquid seed culture medium: by mass percentage, yeast extract powder 1%, glucose 4%, peptone 1%, calcium chloride 0.2%, dipotassium hydrogen phosphate 0.2%.

[0066] Raw materials and inorganic ...

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Abstract

The invention discloses a culture medium for cultivating high-toxicity Metarhizium anisopliae and a preparation of the Metarhizium anisopliae and application of the preparation, and belong to the technical field of plant protection. The culture medium comprises the following components: wheat bran, rice husks, rice flour, corn flour, polypide lyophilized powder, dipotassium hydrogen phosphate, manganese sulfate, calcium chloride and water. Since the polypide lyophilized powder of Spodoptera frugiperda is introduced into a traditional culture medium, the toxicity of Metarhizium anisopliae NG-Ma040 for Spodoptera frugiperda killing can be improved, and no obvious influences on the sporulation quantity can be caused; meanwhile, through synergistic cooperation among the wheat bran, rice husks,rice flour, corn flour, dipotassium hydrogen phosphate, manganese sulfate, calcium chloride and other components, the toxicity for Spodoptera frugiperda is improved further, and the infection rate and control effect on larvae of Spodoptera frugiperda can be improved; and moreover, the culture medium can be made into the preparation of Metarhizium anisopliae, and the preparation is applied to prevention and control of Spodoptera frugiperda.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a medium for cultivating highly virulent Metarhizium anisopliae and its preparation and application. Background technique [0002] Metarhizium anisopliae (Metarhizium anisopliae) belongs to Ascomycota, Hypocreaes, Ergotaceae, and Metarhizium genus, and is a broad-spectrum insecticidal fungus. In nature, it mainly carries out asexual reproduction, and the insect body wall actively invades to infect different insects. It can carry out yeast-like reproduction in the insect hemocoel, produce a large number of insect cells, and can also produce destructin (des tuxin, non-ribosomal polypeptide) Toxoid) and other insecticidal toxins can inhibit insect immunity and accelerate insecticide. It has been found that there are more than 200 kinds of insects that Metarhizium anisopliae can parasitize, and some insecticides have been developed for Metarhizium anisopliae, an...

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14
Inventor 胡飞徐丽娜胡本进苏贤岩任学祥周子燕陈浩梁钟永志苏卫华戚仁德
Owner PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
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