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Solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as preparation method and application of solid fermentation culture medium

A technology of solid fermentation and culture medium, applied in the field of microorganisms, can solve the problems of low spore germination rate, long spore production cycle, low spore production rate, etc., and achieve the effects of increasing spore production, low price, and promoting spore production.

Active Publication Date: 2020-08-18
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the most commonly used medium for cultivating Metarhizium anisopliae is Sars maltose-yeast medium and potato medium, but there are still problems such as low sporulation rate, low spore germination rate, long sporulation cycle and high cost.

Method used

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  • Solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as preparation method and application of solid fermentation culture medium
  • Solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as preparation method and application of solid fermentation culture medium
  • Solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as preparation method and application of solid fermentation culture medium

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preparation example Construction

[0035] The present invention also provides the preparation method of the solid fermentation medium that promotes the spore production of Metarhizium anisopliae described in the above technical scheme, comprising the following steps:

[0036] (1) drying the rice after soaking in water to obtain processed rice;

[0037] (2) After mixing the treated rice obtained in the step (1) with soybeans and supplementary materials, a mixture is obtained, and the mixture is sterilized to obtain a solid fermentation medium.

[0038] In the invention, the rice is soaked in water and then dried to obtain the processed rice. In the present invention, the temperature of the water is preferably 90-100° C., more preferably 95-100° C.; the soaking time is preferably 30-40 minutes, more preferably 33-38 minutes. In the present invention, soaking the rice in water and then drying it preferably includes filtering; the filtering method is preferably gauze filtering; the pores of the gauze are preferabl...

Embodiment 1

[0050] Prepare raw materials: rice 100g, soybean flour 100g and supplementary material 43g, the particle diameter of described soybean flour is 50 orders;

[0051] The supplements include 10 g of lactose, 4 g of glucose, 16 g of yeast powder, 8 g of potassium nitrate and 5 g of peanut oil.

[0052] Follow the steps below to prepare solid fermentation medium:

[0053] First weigh 100g of rice and put it in a large beaker, add 100°C water to soak for 30min, wash it, filter it with 16 mesh (gap) gauze, and dry the surface moisture. Weigh 100g of soybean powder and add it, mix it with 43g of supplementary material, mix evenly, put equal amounts into fresh-keeping bags with a diameter of 15cm and a length of 30cm, steam sterilize at 121°C for 30min, and cool down for later use.

[0054] Follow the steps below to apply:

[0055] (1) Conidia liquid culture stage

[0056] Metarhizium anisopliae was inoculated into solid SMAY medium (maltose 40g / L, peptone 10g / L, yeast extract 10g / L...

Embodiment 2

[0062] Prepare materials by parts by mass: rice 100g, soybean flour 100g and supplementary material 50g, the particle diameter of the soybean flour is 50 mesh;

[0063] The supplements include 10 g of lactose, 6 g of glucose, 16 g of yeast powder, 8 g of potassium nitrate and 10 g of peanut oil.

[0064] Follow the steps below to prepare solid fermentation medium:

[0065] First weigh 100g of rice and put it in a large beaker, add 100°C water to soak for 30min, wash it, filter it with 16 mesh (gap) gauze, and dry the surface moisture. Weigh 100g of soybean powder and add it, mix it with 50g of supplementary material, mix evenly, put equal amounts into fresh-keeping bags with a diameter of 15cm and a length of 30cm, steam sterilize at 121°C for 30min, and cool down for later use.

[0066] Follow the steps below to apply:

[0067] (1) Conidia liquid culture stage

[0068] Metarhizium anisopliae was inoculated into solid SMAY medium (maltose 40g / L, peptone 10g / L, yeast extract...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a solid fermentation culture medium for promoting spore production of metarhizium rileyi as well as a preparation method and application of the solid fermentation culture medium. The solid fermentation culture medium for promoting spore production of metarhizium rileyi is prepared from the following raw materials in parts by mass: 1-3 parts of rice, 1-2 parts of soybeans and 1-2 parts of a supplement, wherein the supplement comprises lactose, glucose, yeast powder, potassium nitrate and peanut oil, andthe mass ratio of lactose to glucose to yeast powder to potassium nitrate to peanut oil is (10-20):(4-8):(16-32):(8-16):(5-10). The spore production quantity of the metarhizium rileyi obtained by adopting the culture medium provided by the invention is greatly improved, the spore production period is short, the spore germination rate reaches 95% or above, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application. Background technique [0002] At this stage, the harm of pests to crops is one of the major factors causing agricultural production reduction. The long-term and extensive use of chemical pesticides has not only killed pests, but also brought a series of side effects such as excessive pesticide residues in agricultural products, increasing pest resistance levels year by year, and polluting the environment. In view of this, people pay more and more attention to biological control, and the use of entomopathogenic microorganisms to control pests is one of the important means of biological control of pests. Compared with other insecticidal microorganisms, insecticidal fungi have the advantages of easy prevalence, environmental friendlines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 丁雪玲何玉仙姚凤銮郑宇
Owner INST OF PLANT PROTECTION FAAS
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