Northern corn leaf blight efficient disease resistance identification inoculant and preparation method thereof
A technology for maize large spot and inoculum, which is applied in the field of plant pathogenic fungi research, can solve the problems of inability to determine single-cell pure strains, unsatisfactory disease resistance identification results, and inconvenient disease resistance identification operations, so as to accelerate growth and improve yield. The amount of spores and the effect of easy mixing and uniformity
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Embodiment 1
[0032] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:
[0033] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.
[0034]Among them, the single-cell purification method adopts the labeling method for single-cell purification. The specific steps are: pour 10ml of thin-layer PDA medium into a petri dish, spread the diluted spore suspension on the medium, and the spore suspension is at 100 The concentration of 1-2 spores per field of view under a magnification lens, seal the cover of the culture dish with a sealing film, and move it into an incubator at 26-...
Embodiment 2
[0041] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:
[0042] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.
[0043] Wherein, the single cell purification method adopts the labeling method for single cell purification, and the specific steps are the same as in Example 1.
[0044] 2) Put the screened highly pathogenic strains into PCA slant medium for 4-7 days and store them in a 4°C refrigerator. The formula of the PCA medium is: 20g of potatoes, 20g of carrots, 16-20g of agar, and 1000ml of water.
[0045] 3) Preparation of secondary strains: Put...
Embodiment 3
[0050] The preparation method of the high-efficiency disease-resistant identification inoculum of maize leaf spot, the steps are as follows:
[0051] 1) For the screening of strong pathogenic strains of corn leaf spot, first isolate the pathogens, then purify them with single cells, use Koch's law to verify the pathogens, and finally screen out the strong pathogenic strains through the determination of the pathogenicity of conventional strains. Virulent strains, high-purity, strong pathogenicity, and high vigor strain HD12 were obtained.
[0052] Wherein, the single cell purification method adopts the labeling method for single cell purification, and the specific steps are the same as in Example 1.
[0053] 2) Put the screened highly pathogenic strains into PCA slant medium for 4-7 days and store them in a 4°C refrigerator. The formula of the PCA medium is: 20g of potatoes, 20g of carrots, 16-20g of agar, and 1000ml of water.
[0054] 3) Preparation of secondary strains: Put...
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