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A solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application

A solid fermentation and culture medium technology, applied in the field of microorganisms, can solve the problems of low spore germination rate, long sporulation cycle, low sporulation rate, etc., and achieve the effect of increasing sporulation, low price, and promoting sporulation

Active Publication Date: 2022-06-14
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the most commonly used medium for cultivating Metarhizium anisopliae is Sars maltose-yeast medium and potato medium, but there are still problems such as low sporulation rate, low spore germination rate, long sporulation cycle and high cost.

Method used

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  • A solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application
  • A solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application
  • A solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application

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preparation example Construction

[0035] The present invention further provides a method for preparing solid fermentation medium to promote spore production of Lychee aeruginosa described in the above technical scheme, comprising the following steps:

[0036] (1) The said rice is soaked in water and dried to obtain processed rice;

[0037] (2) The processed rice obtained in step (1) is mixed with soybeans, supplements, a mixture is obtained, the mixture is sterilized to give a solid fermentation medium.

[0038]The present invention is to dry the rice after soaking in water to give processed rice. In the present invention, the temperature of the water is preferably 90 ~ 100 ° C, more preferably 95 ~ 100 ° C; The soaking time is preferably 30 to 40min, more preferably 33 to 38min. In the present invention, the rice is soaked in water after drying is preferably included filtration; The method of filtration is preferably gauze filtration; The pores of the gauze are preferably 16 to 20 mesh. There are no special restr...

Embodiment 1

[0050] Preparation: 100g of rice, 100g of soybean flour and 43g of supplementary material, the particle size of the soybean flour is 50 mesh;

[0051] The supplement comprises 10g lactose, 4g glucose, yeast powder 16g, potassium nitrate 8g and peanut oil 5g.

[0052] Follow these steps to prepare solid fermentation medium:

[0053]First weigh 100g of rice in a large beaker, add 100 ° C water to soak for 30min, wash, filter with 16 mesh (gap) gauze, dry the surface moisture. Then weigh 100g of soybean flour to add, mix in 43g of supplement, mix evenly, equal amounts in a 15cm diameter, 30cm long crisp bag, 121 °C steam sterilization for 30min, cooling and standby.

[0054] Follow these steps to apply:

[0055] (1) Conidia liquid culture stage

[0056] Inoculate Lychee aerantes into solid SMAY medium (maltose 40 g / L, peptone 10 g / L, yeast extract 10 g / L, agar 18 g / L), placed in a 28 °C light incubator (16 L / 8D, alternating light and dark), and grow conidia after 7 days of incubation...

Embodiment 2

[0062] Prepare according to the mass portion: 100g of rice, 100g of soybean flour and 50g of supplementary material, the particle size of the soybean flour is 50 mesh;

[0063] The supplement comprises 10g lactose, 6g glucose, yeast powder 16g, potassium nitrate 8g and peanut oil 10g.

[0064]Follow these steps to prepare solid fermentation medium:

[0065] First weigh 100g of rice in a large beaker, add 100 ° C water to soak for 30min, wash, filter with 16 mesh (gap) gauze, dry the surface moisture. Then weigh 100g of soybean flour to add, mix in 50g of supplement, mix evenly, equal amounts in a 15cm diameter, 30cm long crisp bag, 121 °C steam sterilization for 30min, cooling and standby.

[0066] Follow these steps to apply:

[0067] (1) Conidia liquid culture stage

[0068] Inoculate Lychee aerantes into solid SMAY medium (maltose 40 g / L, peptone 10 g / L, yeast extract 10 g / L, agar 18 g / L), placed in a 28 °C light incubator (16 L / 8D, alternating light and dark), and grow conidia...

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a solid fermentation medium for promoting spore production of Metarhizium anisopliae and its preparation method and application. A solid fermentation medium for promoting spore production of Metarhizium anisopliae, prepared from the following raw materials in parts by mass: 1-3 parts of rice, 1-2 parts of soybeans and 1-2 parts of supplementary materials; the supplementary materials include Lactose, glucose, yeast powder, potassium nitrate and peanut oil, the mass ratio of the lactose, glucose, yeast powder, potassium nitrate and peanut oil is (10~20):(4~8):(16~32):(8~ 16): (5~10). The sporulation yield of the metarhizium anisopliae obtained by adopting the culture medium provided by the invention is greatly improved, the sporulation period is short, the spore germination rate reaches over 95%, and the cost is low.

Description

Technical field [0001] The present invention belongs to the field of microbial technology, specifically relates to a solid fermentation medium to promote the production of Spores of Lychee agresscii and preparation methods and applications thereof. Background [0002] At this stage, the harm of pests to crops is one of the major factors causing agricultural production reduction. The long-term large-scale use of chemical pesticides has also brought about a series of side effects such as excessive pesticide residues in agricultural products, increased pest resistance levels year by year, and polluted the environment. In view of this, biological control is increasingly valued, and the use of insect pathogenic microorganisms to control pests is one of the important means of biological control of pests. Compared with other insecticidal microorganisms, insecticidal fungi have the advantages of being easy to circulate, friendly to the environment, and not easy to produce resistance, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 丁雪玲何玉仙姚凤銮郑宇
Owner INST OF PLANT PROTECTION FAAS
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