Method for promoting spore production of monascus through co-culture of saccharomycetes and monascus
A technology of Monascus and yeast, which is applied in the field of microbial reproduction, can solve the problems of weak spore production ability of Monascus, and it is difficult to significantly increase the spore production of Monascus, so as to increase the spore production of Monascus, reduce adverse effects, and promote The effect of Monascus sporulation
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Embodiment 1
[0029] The co-cultivation of a kind of yeast and Monascus of the present embodiment promotes the method for sporulation of Monascus, comprising the steps:
[0030] (1) Preparation of seed solution
[0031] Preparation of two kinds of yeast seed liquids: with 2% inoculation amount, the SHBCC D54233 bacterial strain (directly purchased) and the ATCC 58045 bacterial strain (directly purchased) were inoculated into the McFarland medium, and shake culture at 30°C, 150r / min for a period of time for later use. Among them, the formula of McFarland's medium is: glucose 1g, yeast extract powder 2.5g, anhydrous sodium acetate 8.2g, potassium chloride 1.8g, fixed volume in 1L water; the shaking culture time of SHBCC D54233 strain is 20h, ATCC 58045 strain The shaking culture time is 32h.
[0032] Preparation of Monascus seed liquid: the concentration was 5×10 with 3% inoculum 6 The CFU / mL Monascus spore suspension was inoculated into the Monascus seed medium, and cultured with shaking ...
Embodiment 2
[0037] This embodiment is used to verify the impact of important parameters in the above-mentioned embodiment 1 method:
[0038] 1. Effect of co-cultivation of two strains of yeast and Monascus on spore production:
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