Alternaria alternate spore suspension liquid and application thereof
A technology of tobacco red spot bacteria and spore suspension, which is applied in the field of agricultural microorganisms, can solve the problems of difficult control of ultraviolet dose and time, low mycelial sporulation efficiency, and culture pollution, so as to achieve shortened culture period, large inoculum amount, and guaranteed The effect of cultivating quality
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[0019] The medium described in step B is oat medium (OA), potato dextrose medium (PDA), potato sucrose medium (PSA), Czapek’s medium or Richard’s medium.
[0020] The solution for promoting spore germination described in step D is one or more combinations of 1-2% sterilized glucose, fructose, sucrose, and peptone solutions. Alternatively, the spore germination-promoting solution described in step D is 2-5% fresh mature tobacco leaf tissue mash liquid of susceptible varieties of tobacco, and the tissue mash liquid is sterilized by filtration. The susceptible varieties of tobacco are K326, Yunyan 85 or G140.
[0021] The tissue pounding described in step D adopts a mortar, pounder or grinder.
[0022] The filtering described in step D is filtering with absorbent cotton or 4-6 layers of absorbent gauze.
[0023] The application of the tobacco brown spot fungus spore suspension in the present invention in the determination of tobacco variety resistance to black spot disease is t...
Embodiment 1
[0029] Tobacco diseased leaves with typical symptoms were collected from diseased fields, and strains of Alternaria Alternaria were screened out through separation, purification, and pathogenicity measurement; the hyphae producing spores of the strains were spotted on the culture medium plate, and each plate was Inoculate at 3 points, under the condition of 18h dark and 6h alternating light, culture at 27°C for 4 days, select the culture with dark brown mycelium and denser mycelium for inducing sporulation; plate the culture medium that meets the conditions for inducing sporulation , placed in a refrigerator at 2°C in a sealed state for induction culture in the dark for 2 days, then transferred to an incubator for dark, gradient cooling culture, and cooled from 20°C to 15°C over 2 days (the cooling rate is about 1°C every 9.6h); induction After the cultivation is completed, use 1% sterilized glucose solution as the solution for promoting spore germination, wash off the spores a...
Embodiment 2
[0031] Tobacco diseased leaves with typical symptoms were collected from diseased fields, and strains of Alternaria Alternaria were screened out through separation, purification, and pathogenicity measurement; the hyphae producing spores of the strains were spotted on the culture medium plate, and each plate was Inoculate at 4 points, under the condition of 18h dark and 6h alternating light, culture at 29°C for 6 days, select the culture with dark brown mycelium and denser mycelium to induce sporulation; plate the culture medium that meets the conditions for sporulation induction , placed in a refrigerator at 4°C in a sealed state for induction culture in the dark for 3 days, then transferred to an incubator for dark, gradient cooling culture, and the temperature was lowered from 20°C to 15°C over 3 days (the cooling rate was about 1°C every 14.4h); induction After the cultivation is completed, use 2% sterilized fructose and sucrose solution as a solution to promote spore germi...
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