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Fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use method

A technology of Metarhizium anisopliae and fermentation medium, which is applied in the field of culture medium, can solve problems such as application limitations of Metarhizium anisopliae, and achieve the effects of proper ingredients and ratio, short production cycle, and simple preparation method

Active Publication Date: 2018-03-27
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to various problems in fermentation methods, formulation development and environmental constraints, the application of Metarhizium anisopliae in pest biological control was once greatly restricted. Since 1879, Mehhnhob first mass-produced Metarhizium anisopliae to control Austrian scarabs ( Anisopliae austriaca Since Herbst), the application of Metarhizium anisopliae in the biological control of pests has gradually started.

Method used

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  • Fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use method
  • Fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use method
  • Fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use method

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Embodiment Construction

[0020] The fermented medium suitable for producing sporulation of Metarhizium anisopliae comprises seed medium and solid fermentation medium, wherein said seed medium comprises solid seed medium and liquid seed medium; Containing mass concentration in every liter of solid seed medium is 20% potato juice 1000mL, glucose 20g, KH 2 PO 4 3g, MgSO 4 1.5g and 20g of agar, the balance is water; each liter of liquid seed medium contains 10 g of peptone, 10 g of sucrose, K 2 HPO 4 0.3 g, MgSO 4 0.3 g and NaCl 0.3 g, the balance is water;

[0021] The components of the solid fermentation medium are: wheat bran, corn grits and distilled water; wherein the mass ratio of wheat bran and corn grits is 1:1, and the mass of distilled water is 55-60% of the total mass of wheat bran and corn grits. The preparation method of the solid fermentation medium is as follows: take wheat bran and corn grits with a mass ratio of 1:1, mix them evenly, put them into a triangular flask, add distille...

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Abstract

The fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use methods relate to a medium; the medium includes a seed medium and a solid fermentation medium. Described seed culture medium comprises solid seed culture medium and liquid seed culture medium; Contain potato juice 1000mL, glucose, KH2PO4, MgSO4 and agar in the solid seed culture medium, surplus is water; Contain peptone, sucrose, K2HPO4 in the liquid seed culture medium , MgSO4 and NaCl, and the balance is water; the composition of the solid fermentation medium is: wheat bran, corn grits and distilled water; wherein the mass ratio of wheat bran and corn grits is 1:1, and the quality of distilled water is wheat bran and corn grits The total mass of grits is 55‑60%. The fermentation medium suitable for the sporulation of the Metarhizium anisopliae of the present invention is suitable for the growth of the Metarhizium anisopliae, and can produce more than 1×109 spores per gram of fermentation raw material; the germination rate of the prepared spore powder can reach 95%.

Description

technical field [0001] The invention relates to a medium, specifically a fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use methods. Background technique [0002] Metarhizium anisopliae ( Metarhizium sp.) is a broad-spectrum entomogenic fungus, which can multiply in large numbers in the body cavity after invading the body wall of insects, causing insect death. Because Metarhizium anisopliae has strong pathogenicity to insects, is non-toxic to humans, livestock, and crops, is safe to use, and has a long control effect, Metarhizium anisopliae has a good application prospect as a biopesticide. However, due to various problems in fermentation methods, formulation development and environmental constraints, the application of Metarhizium anisopliae in pest biological control was once greatly restricted. Since 1879, Mehhnhob first mass-produced Metarhizium anisopliae to control Austrian scarabs ( Anisopliae austriaca Since Herbst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 侯颖徐建强林晓民刘圣明李晓艳
Owner HENAN UNIV OF SCI & TECH
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