Fermentation medium suitable for sporulation of Metarhizium anisopliae and its preparation and use method
A technology of Metarhizium anisopliae and fermentation medium, which is applied in the field of culture medium, can solve problems such as application limitations of Metarhizium anisopliae, and achieve the effects of proper ingredients and ratio, short production cycle, and simple preparation method
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[0020] The fermented medium suitable for producing sporulation of Metarhizium anisopliae comprises seed medium and solid fermentation medium, wherein said seed medium comprises solid seed medium and liquid seed medium; Containing mass concentration in every liter of solid seed medium is 20% potato juice 1000mL, glucose 20g, KH 2 PO 4 3g, MgSO 4 1.5g and 20g of agar, the balance is water; each liter of liquid seed medium contains 10 g of peptone, 10 g of sucrose, K 2 HPO 4 0.3 g, MgSO 4 0.3 g and NaCl 0.3 g, the balance is water;
[0021] The components of the solid fermentation medium are: wheat bran, corn grits and distilled water; wherein the mass ratio of wheat bran and corn grits is 1:1, and the mass of distilled water is 55-60% of the total mass of wheat bran and corn grits. The preparation method of the solid fermentation medium is as follows: take wheat bran and corn grits with a mass ratio of 1:1, mix them evenly, put them into a triangular flask, add distille...
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