Method for continuously culturing bacillus subtilis and special fermenting system
The technology of a Bacillus subtilis and its cultivation method is applied to the cultivation of Bacillus subtilis and the field of special equipment in the cultivation process, which can solve the problems of low spore formation rate and high production cost, achieve stable and uniform quality, prolong life, damage reduction effect
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Embodiment 1
[0065] A fermentation system dedicated to the continuous culture method of Bacillus subtilis, including a batching tank 1, a continuous elimination device 2, a maintenance tank 3, a cooling device 11, a feed liquid storage tank 5, a primary culture tank 6, and a secondary culture tank sequentially connected in series 8. Spore culture tank 9;
[0066] A seed tank 7 is connected in parallel between the cooling device 11 and the primary culture tank 6;
[0067] The spore culture tank 9 is connected with an auxiliary material tank 10;
[0068] A delivery pump I4 is connected between the batching tank 1 and the continuous elimination device 2; a delivery pump II12 is connected between the maintenance tank 3 and the cooling device 11;
[0069] There is a constant flow pump between the material storage tank, the primary cultivation tank 6, the secondary cultivation tank 8 and the spore cultivation tank 9.
[0070] Concrete steps are, inoculate 0.5-1% Bacillus subtilis bacterial cla...
Embodiment 2
[0074] The continuous culture method of bacillus subtilis comprises the following steps:
[0075] (1) Seed cultivation
[0076] a. Activation of strains on test tube slant
[0077] Transfer the preserved Bacillus subtilis strains to the test tube slant medium, keep at 33°C, and cultivate for 30 hours to activate the strains;
[0078] The formula of the test tube slant medium is: glucose 4g / L, peptone 3g / L, NaCl 1g / L, beef extract 0.5g / L, agar 20g / L, pH7.1, sterilized at 121°C for 20 minutes;
[0079] b. Erlenmeyer flask seed culture
[0080] Pick 1-2 rings of Bacillus subtilis strains from the activated test tube and inoculate them into the Erlenmeyer flask, keep at 33°C, 120rpm after inoculation, and cultivate for 17 hours;
[0081] The medium formula of the triangular flask is: glucose 20g / L, peptone 15g / L, NaCl 5g / L, beef extract 0.5g / L, pH7.1, sterilized at 121°C for 30 minutes;
[0082] c. Seed tank culture
[0083] Inoculate the culture material A in the seed tank b...
Embodiment 3
[0105] The continuous culture method of bacillus subtilis comprises the following steps:
[0106] (1) Seed cultivation
[0107] a. Activation of strains on test tube slant
[0108] Transfer the preserved Bacillus subtilis strains to the test tube slant medium, keep at 32°C, and cultivate for 24 hours to activate the strains;
[0109] The formula of test tube slant medium is: glucose 4g / L, peptone 3g / L, NaCl 1g / L, beef extract 0.5g / L, agar 20g / L, pH7.0, sterilized at 121°C for 20 minutes;
[0110] b. Erlenmeyer flask seed culture
[0111] Pick 1-2 rings of bacteria from the activated test tube and inoculate them into the Erlenmeyer flask, keep at 32°C, 100rpm after inoculation, and cultivate for 16 hours;
[0112] The medium formula of the Erlenmeyer flask is: glucose 20g / L, peptone 15g / L, NaCl 5g / L, beef extract 0.5g / L, pH 7.0, sterilized at 121°C for 30 minutes;
[0113] c. Seed tank culture
[0114] Inoculate the culture material A in the seed tank by inoculating the ab...
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