31results about How to "Less prone to pollution" patented technology

The invention discloses a method for culturing Pleurotus ferulae through corncob, which includes the following process: preparing materials, bagging, sterilizing, culturing, building wall and earthing, and then outputting; the method for culturing Pleurotus ferulae through corncob has the advantages that the biology transformation ratio of the Pleurotus ferulae is improved from 40-50 percent to 80-85 percent and the production cost is reduced from the original cost about 1.32 Yuan/bag to the cost about 1.08 Yuan/bag; the costing product quality of the Pleurotus ferulae is obviously improved and the taste is more excellent; the method for culturing Pleurotus ferulae through corncob after improvement is advanced in technology; the process is simple and rational; Pleurotus ferulae bags are not easy to be polluted; the finished product rate is high; the positivity of farmers to culture the Pleurotus ferulae is higher and the profit is more obvious.

The invention belongs to the field of microbial fermentation engineering, and specifically relates to a high-density culture method of clostridium thermocellum. The method is characterized in that a GS-2 culture medium is used as an initial culture medium; the fermentation medium is optimized through a multivariate statistical method; and cell concentration of clostridium thermocellum is rapidly and efficiently raised. The invention has the following advantage: the invention provides a fermentation medium for rapid acquisition of large cell concentration of clostridium thermocellum, thus laying the groundwork for subsequent production of cellulosome which has high conversion efficiency of cellulose to bioethanol. Bacterial density of clostridium thermocellum is raised by 6-9 times in comparison with that of the initial culture medium. OD600 under the bottling condition reaches 8.85, and OD600 in a fermentation cylinder reaches 12.7.

The invention relates to an open type simplified culture medium for taxus cuspidate. The culture medium comprises a primary culture medium and a subculture medium. The primary culture medium is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, active carbon AC and agricultural streptomycin into optimized B5 basic mother liquor. The subculture is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, 6-benzyl amino adenine BA, active carbon AC and agricultural streptomycin into optimized MS basic mother liquor. According to the culture medium provided by the invention, with the adoption of open type simplified tissue culture, the cost and the inoculating time of the culture medium are remarkably saved, the autoclaved sterilization program is cancelled, and the production flow is simplified by replacing autoclaved sterilization and strict enclosed environment operation by a bacteriostatic agent, so that the labor and equipment investment in industrialized seedling production can be reduced, and the fund is saved by 30-50%.

The invention relates to the field of nutrient solution bottle cleaning equipment, and discloses a cup and bottle cleaning machine which comprises a supporting rack, a conveying device, a driving plate device, a bottle opening cleaning device and a bottle body cleaning device. The supporting rack comprises a supporting frame and a workbench, the workbench is fixed on the supporting frame, the workbench is provided with a conveying channel, the conveying device is connected with one end of the conveying channel, the driving plate device, the bottle opening cleaning device and the bottle body cleaning device are fixed on the workbench, the conveying channel penetrates through the driving plate device, the bottle opening cleaning device and the bottle body cleaning device in sequence, the bottle opening cleaning device is used for cleaning bottle openings of nutrient solution bottles, and the bottle body cleaning device is used for cleaning bottle bodies of the nutrient solution bottles.When the nutrient solution bottle is cleaned, the bottle opening and the bottle body of the nutrient solution bottle are separately cleaned so that the cleaned nutrient solution bottle is cleaner.

The invention discloses a preparation method of skin fibroblasts, and belongs to the technical field of biological cells. The preparation method of the skin fibroblasts comprises the following steps of: S01, specimen treatment: treating a skin specimen, and removing adipose tissues and connective tissues; S02, separation: treating the treated specimen with neutral protease, and removing epidermis to obtain dermis tissues; S03, digestion: digesting the dermis tissues by using pancreatin to obtain tissue fluid; S04, centrifugation: centrifuging the tissue fluid, and removing supernatant to obtain a cell suspension; S05, culture: transferring the cell suspension into a culture dish, adding a culture solution, and culturing; and S06, passage: culturing until the fusion degree is 75-85%, and performing passage culture. The preparation method disclosed by the invention has the advantages of simple steps, low possibility of pollution, a high cell survival rate, a good purification effect and small cell loss.

The invention provides a method for treating heavy metal wastewater by virtue of a conducting film. The method comprises the following specific steps: 1, pumping heavy metal wastewater entering from a wastewater inlet (1) to a pre-treatment device (3) through a pump (2) to remove particles in wastewater; 2, introducing the wastewater without particles to a pH adjusting pool (4) to adjust the pH value of the wastewater to 4-6.5; and 3, pumping the wastewater with adjusted pH value to a film pond (9) through a valve (5), a flowmeter (6) and a pressure gauge (7) by means of a pump (2'), wherein heavy metal ions can not penetrate through a conducting film to form heavy metal concentrated liquor due to the positive electricity rejection effect between the conducting film as an anode in the film pool (9) and the heavy metal ions in the wastewater, while water in the wastewater penetrates through the conducting film to form a penetrating fluid. The heavy metal concentrated liquor flows back to the adjusting pool (4) through a pressure gauge (7') and a valve (5') while the penetrating fluid is discharged through a penetrating fluid outlet (8).

The invention relates to the technical field of nuclear fuel aftertreatment and particularly discloses a device for measuring americium and cesium in aftertreatment feed liquid. A NaI detector is arranged in an area defined by central holes of a lead sheath A, a lead sheath B and a lead sheath D, and a connecting line of the NaI detector penetrates out of a slot in the lead sheath B to be connected with a multi-channel spectrometer; the multi-channel spectrometer is connected with a computer through a data line; and gamma ray signals are converted into electric signals by the NaI detector, theelectric signals are screened by the multi-channel spectrometer and then transmitted into the computer, and automatic sample measuring is realized. The device can effectively shield interference of environmental gamma rays in a measuring system, the multi-channel spectrometer is utilized, thus the gamma radioactivity concentrations of cesium-137 and americium-241 can be measured simultaneously, and the measuring result is directly read by software.

The invention discloses a special thermophilic bacterium for catalytic production of alpha-ketoglutaric acid and a catalytic production method. The strain is Streptomyces sp.TC2303 and is preserved in the China Center for Type Culture Collection on December 27, 2021, the preservation date is December 27, 2021, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20211682. The culture and fermentation conditions of the strain Streptomyces sp.TC2303 provided by the invention are both about 45 DEG C, the fermentation time is only 24 hours, and the fermentation time is short; according to the present invention, the fermentation broth can be used to catalyze L-glutamic acid at the temperature of 45-56 DEG C to produce alpha-ketoglutaric acid, the conversion process temperature is more than 45 DEG C, the catalysis temperature is far higher than the prior art, the total reaction time is only 18 h, the catalysis process is not easily subjected to bacterial contamination, and the method has special advantages for enterprises with simple and crude equipment and easy bacterial contamination.

The invention relates to the field of application of medical instruments and discloses a tissue filtering method for extracting cartilage cells, the method is carried out in a tissue filtering device, the tissue filtering device comprises a tubular part (1), a filter screen (2) and a clamping part (3); the filter screen (2) is arranged at one end of the tubular part (1) and covers an opening located at one end of the tubular part (1); the clamping part (3) is arranged on the inner wall of the other end of the tubular part (1), and the clamping part (3) is arranged to be convenient for a user to clamp and fix by using a corresponding clamp. The tissue filtering device applied in the method has the advantages of being low in cost, convenient to operate and not prone to pollution.

The invention belongs to the field of microbes, and particularly relates to a Bacillus licheniformis continuous culture method and a special fermentation system. The method comprises the following steps: (1) inoculating a Bacillus licheniformis strain into a test tube slant culture medium, activating the strain, sequentially inoculating into a triangular flask a seeding tank, and culturing, thereby preparing the Bacillus licheniformis seeds; (2) taking a culture medium composed of starch/glucose, corn flour, bean pulp powder, (NH4)2HPO4, NaCl, CaCl2, MgSO4.7H2O, KH2PO4, K2HPO4, MnSO4, a defoaming agent and water; and (3) sterilizing the culture medium in the step (2), inoculating and activating the cultured Bacillus licheniformis seeds, and carrying out three-stage continuous culture, thereby obtaining the final fermentation liquid. The method implements high mechanization and automation, and the Bacillus licheniformis has stable and uniform quality and can not have the phenomena of infectious microbe pollution and strain variation. The method prolongs the service lives of detecting instruments, saves the time, enhances the yield and production efficiency, and increases the Bacillus licheniformis strain concentration and spore rate.

The invention discloses an electrode for preparing a piece of cover glass. The electrode comprises an electrode containing an additive, the electrode is installed at a pool wall of a melting pool, andthe other side of the electrode is provided with a water cooling plate; the electrode employs stannic oxide as a base-material, the additive comprises antimony oxide, zirconium oxide and copper oxide, and the mass of the zirconium oxide is 1.8-2.2% of the total mass of the electrode. The electrode for preparing the cover glass comprises the electrode containing the additive, the electrode is installed at a pool wall of a melting pool, the other side of the electrode is provided with a water cooling plate; the electrode employs stannic oxide as a base-material, the additive comprises antimonyoxide, zirconium oxide and copper oxide, ZrO2 is added into the stannic oxide electrode material, so that the electrode has higher usage temperature, high resistance to oxidation, and better erosion resistance; the product can be operated at 1600 DEG C for a long time, service lives of the electrode and a tank furnace are prolonged, compactness of the electrode material is increased, and falling of blocks and pollution on glass liquid are not easy to happen in production.

The invention relates to the technical field of fluorescence polarization, in particular to a method suitable to detect mononucleotide polymorphism in a fluorescence polarization technology. In order to overcome the problems of high cost, complex operation step, easy pollution and detection result influence in the prior art, the technical scheme is achieved as follows: reagents adopted by the method comprise MgCl2, 10* reaction buffer, TapDNA polymerase, dNTPs, target gene DNA primer, and a DNA probe with fluorescence labeling at the 3 end of a target zone; and the reagents are performed with amplified reaction. The invention has the advantages that: firstly, the steps and the operation are simple, the amplified reaction is performed in a hybrid stopped tube by one step without easy pollution and accurate result; secondly, the result analysis is simple and the result is judged by only number comparison so as to be easy to standardize and automate, and the application range is wide; thirdly, the cost is low without any special reagent and fluorescent quenching or microgroove wedding agent.

The invention provides an SMT material suction device. The SMT material suction device comprises a first support and at least one material suction assembly arranged on the first support side by side, wherein each material suction assembly comprises a first mounting block, a first suction nozzle arranged on the first mounting block and a first driving mechanism pushing the first mounting block to move up and down; the first support is provided with a first guide mechanism matched with the first mounting block for up-down movement; and the first driving mechanism is in floating connection with the first mounting block through a first connecting block. Products can be automatically placed on the corresponding carrier tape or the packaging tray to be packaged, so that operation is convenient, the speed is high, and manpower and material resources are saved; and the first driving mechanism is in floating connection with the first mounting block through the first connecting block, and the connecting position of the first connecting block and the first mounting block can be properly and automatically adjusted, so that the safety and the reliability are high, the production efficiency and the yield of products are improved, the production control and the automatic online production of the products are facilitated, and the production cost is reduced.