The invention discloses an expanded artificial
scaffold protein, an artificial fibrosome and a preparation method and application thereof, and belongs to the technical field of
enzyme engineering. According to the invention, the
scaffold proteins containing different numbers of adhesion modules are designed, and the two
scaffold proteins are modularly assembled by using a covalent linkage
system, so that the oversized artificial
scaffold protein of which the number of adhesion modules can reach 19 is successfully constructed, and the scale of the oversized artificial
scaffold protein exceeds that of the known natural
scaffold protein. The scaffold
protein can efficiently carry various catalytic subunits through specific interaction of an adhesion module and a docking module to form an artificial fibrosome. Experiments show that the artificial
cellulosome can efficiently degrade
cellulose substrates such as crystalline
cellulose and the like especially when the scaffold
protein contains an extremely high amount of cohesin. The invention provides a novel efficient
enzyme complex tool for efficiently degrading lignocellulose
biomass, and has a wide application prospect in the fields of
biofuel, bio-based
chemical production and the like.