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3 results about "Cellulosome" patented technology

Cellulosomes are multi-enzyme complexes. Cellulosomes are associated with the cell surface and mediate cell attachment to insoluble substrates and degrade them to soluble products which are then absorbed. Cellulosome complexes are intricate, multi-enzyme machines, produced by many cellulolytic microorganisms. They are produced by microorganisms for efficient degradation of plant cell wall polysaccharides, notably cellulose, the most abundant organic polymer on Earth. The multiple subunits of cellulosomes are composed of numerous functional domains which interact with each other and with the cellulosic substrate. One of these subunits, a large glycoprotein "scaffoldin", is a distinctive class of non-catalytic scaffolding polypeptides. The scaffoldin subunit selectively integrates the various cellulases and xylanase subunits into the cohesive complex, by combining its cohesin domains with a typical dockerin domain present on each of the subunit enzymes. The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module that adheres cellulose to the cellulosomal complex.

Expanded artificial scaffold protein, artificial fibrosome, preparation method and application

The invention discloses an expanded artificial scaffold protein, an artificial fibrosome and a preparation method and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, the scaffold proteins containing different numbers of adhesion modules are designed, and the two scaffold proteins are modularly assembled by using a covalent linkage system, so that the oversized artificial scaffold protein of which the number of adhesion modules can reach 19 is successfully constructed, and the scale of the oversized artificial scaffold protein exceeds that of the known natural scaffold protein. The scaffold protein can efficiently carry various catalytic subunits through specific interaction of an adhesion module and a docking module to form an artificial fibrosome. Experiments show that the artificial cellulosome can efficiently degrade cellulose substrates such as crystalline cellulose and the like especially when the scaffold protein contains an extremely high amount of cohesin. The invention provides a novel efficient enzyme complex tool for efficiently degrading lignocellulose biomass, and has a wide application prospect in the fields of biofuel, bio-based chemical production and the like.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Method for saccharifying and pretreating bagasse based on charcoal-reinforced cellulosome synergistic fungal cellulase and application

The invention discloses a method for saccharifying and pretreating bagasse based on charcoal-reinforced cellulosome in cooperation with fungal cellulase and application. According to the method, biochar is used for regulating and controlling cellulose degrading bacteria to efficiently synthesize small fibers in a lignocellulose-containing culture medium, and the biochar and low-load fungal cellulase are combined for application, so that efficient saccharification of pretreated bagasse is realized. By introducing the biochar, the metabolic activity of thalli can be enhanced, accumulation of cellulosome is promoted, and the cellulose degradation rate and the sugar yield are remarkably improved through the synergistic effect of the cellulosome and cellulase, so that the efficient sugar production effect is achieved while the usage amount of the cellulase is reduced. The invention provides a novel green, low-carbon and cost-controllable biomass waste saccharification treatment approach, provides an efficient and feasible technical scheme for lignocellulose resource utilization and biomass energy conversion, and has a good industrial application prospect.
Owner:SOUTH CHINA UNIV OF TECH

Enzyme synergistic surface-displayed artificial cellulosome, its construction method and whole-cell catalytic application

The application discloses an enzyme synergistic effect type surface display artificial fiber organelle, a construction method thereof and whole cell catalysis application. The application takes BL21 (DE3) Delta CsgA as a chassis, eliminates assembly interference by knocking out endogenous CsgA through CRISPR-Cas9, constructs a recombination vector, carries out modular fusion on CsgA anchoring protein, GH10 family xylanase and GH5 family bifunctional glucanase, and realizes efficient display of self-assembly fiber organelle on the surface of escherichia coli. It is verified that the fiber organelle is correctly assembled and clearly positioned. The constructed engineering bacteria can be used as a whole cell catalyst to directly degrade lignocellulose substrates such as corn, rice and peanut straw, and generate functional oligosaccharides with a polymerization degree of 3-4. The application has the advantages of simple process, low cost, strong catalytic synergy, specific product and the like, and can be used in the fields of biomass resource utilization, feed anti-nutritional factor removal, functional oligosaccharide preparation and the like.
Owner:ZHEJIANG UNIV