4 results about "Cellulosome" patented technology

Aiming at the current situation that the saccharification efficiency of lignocellulose is difficult to further improve due to low degradation rates of hemicellulose and xylan in an integrated biological saccharification process in the prior art, the invention firstly provides application of a specific bifunctional enzyme CcXylGH43 in integrated biological saccharification of clostridium thermocellum, the bifunctional enzyme CcXylGH43 is from Clostridium thermocellum, and the nucleotide sequence of the bifunctional enzyme CcXylGH43 is shown as SEQ ID NO: 1. After the specific bifunctional enzyme CcXylGH43 is expressed in clostridium thermocellum, the specific bifunctional enzyme CcXylGH43 not only has good enzyme activity, but also has a synergistic effect with cellulosome, so that a remarkable promotion effect is generated on saccharification, and an unexpected technical effect is realized. On the basis, the invention provides a construction method of three clostridium thermocellum recombinant strains for expressing a bifunctional enzyme CcXylGH43, and a method for carrying out whole-cell saccharification on a lignocellulose substrate by adopting the clostridium thermocellum recombinant strains. The recombinant strain is used for integrated biological saccharification, the saccharification product only contains glucose and xylose, zero accumulation of xylo-oligosaccharide is realized, and compared with the prior art, the progress is remarkable; meanwhile, the yield of xylose and the yield of glucose are increased, and the method has important significance on industrial application.

The invention discloses an expanded artificial scaffold protein, an artificial fibrosome and a preparation method and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, the scaffold proteins containing different numbers of adhesion modules are designed, and the two scaffold proteins are modularly assembled by using a covalent linkage system, so that the oversized artificial scaffold protein of which the number of adhesion modules can reach 19 is successfully constructed, and the scale of the oversized artificial scaffold protein exceeds that of the known natural scaffold protein. The scaffold protein can efficiently carry various catalytic subunits through specific interaction of an adhesion module and a docking module to form an artificial fibrosome. Experiments show that the artificial cellulosome can efficiently degrade cellulose substrates such as crystalline cellulose and the like especially when the scaffold protein contains an extremely high amount of cohesin. The invention provides a novel efficient enzyme complex tool for efficiently degrading lignocellulose biomass, and has a wide application prospect in the fields of biofuel, bio-based chemical production and the like.

The invention discloses a method for saccharifying and pretreating bagasse based on charcoal-reinforced cellulosome in cooperation with fungal cellulase and application. According to the method, biochar is used for regulating and controlling cellulose degrading bacteria to efficiently synthesize small fibers in a lignocellulose-containing culture medium, and the biochar and low-load fungal cellulase are combined for application, so that efficient saccharification of pretreated bagasse is realized. By introducing the biochar, the metabolic activity of thalli can be enhanced, accumulation of cellulosome is promoted, and the cellulose degradation rate and the sugar yield are remarkably improved through the synergistic effect of the cellulosome and cellulase, so that the efficient sugar production effect is achieved while the usage amount of the cellulase is reduced. The invention provides a novel green, low-carbon and cost-controllable biomass waste saccharification treatment approach, provides an efficient and feasible technical scheme for lignocellulose resource utilization and biomass energy conversion, and has a good industrial application prospect.

The application discloses an enzyme synergistic effect type surface display artificial fiber organelle, a construction method thereof and whole cell catalysis application. The application takes BL21 (DE3) Delta CsgA as a chassis, eliminates assembly interference by knocking out endogenous CsgA through CRISPR-Cas9, constructs a recombination vector, carries out modular fusion on CsgA anchoring protein, GH10 family xylanase and GH5 family bifunctional glucanase, and realizes efficient display of self-assembly fiber organelle on the surface of escherichia coli. It is verified that the fiber organelle is correctly assembled and clearly positioned. The constructed engineering bacteria can be used as a whole cell catalyst to directly degrade lignocellulose substrates such as corn, rice and peanut straw, and generate functional oligosaccharides with a polymerization degree of 3-4. The application has the advantages of simple process, low cost, strong catalytic synergy, specific product and the like, and can be used in the fields of biomass resource utilization, feed anti-nutritional factor removal, functional oligosaccharide preparation and the like.