Pichia pastoris surface co-display system based on cellulosome as well as construction method and application of pichia pastoris surface co-display system

A technology of surface co-display and Pichia pastoris, applied in the fields of application, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of difficult foreign protein display efficiency quantification, low amino acid sequence homology, etc., and achieve improvement The effect of display ability, improvement of display ability, and cost saving of preparation
CN110229220AInactive Publication Date: 2019-09-13NANJING FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING FORESTRY UNIV
Publication Date
2019-09-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a pichia pastoris surface co-display system based on cellulosome as well as a construction method and application of the pichia pastoris surface co-display system and belongs tothe technical fields of molecular biology, food and gene engineering. The system comprises anchoring protein and polyprotein display chassis protein, wherein the anchoring protein is saccharomyces cerevisiae cell wall protein Sed1p; and the polyprotein display chassis protein is artificial mini scaffold protein MixA2 of clostridium cellulosome. The pichia pastoris surface co-display system basedon cellulosome can realize polyprotein co-display on cell surfaces; heterologous protein for fusion expression of dockerin is prepared from recombinant bacteria, purification is not needed, multiple proteins can be displayed on cell surfaces by direct specific binding of dockerin on recombinant protein and cohesion on MixA2, and the preparation cost of recombinant protein can be saved effectively.
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Description

technical field

[0001] The invention belongs to the technical fields of molecular biology, food and genetic engineering, and in particular relates to a cellosome-based co-display system on the surface of Pichia pastoris and its construction method and application. Background technique

[0002] Microbial cell surface display is introduced into microbial host cells by fusing the target protein gene sequence with a specific carrier protein gene sequence. The target protein is expressed and positioned on the microbial cell surface under the guidance of the carrier protein, maintaining a relatively independent spatial conformation and original biological activity. The surface display system has the advantages of high efficiency and safety, and is a good platform for expressing foreign proteins. In recent years, its application has become more and more extensive. Whole-cell catalysts; displaying foreign proteins on the cell surface and discovering substances that interact with th...

Claims

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