Preparation method of skin fibroblasts

A fibroblast and skin technology, applied in the field of skin fibroblast preparation, can solve the problems of product purity, cell death, and poor cell activity, so as to facilitate subsequent separation and movement, reduce cell damage, and ensure cell integrity. The effect of the activity

Pending Publication Date: 2021-11-09
郑州源创基因科技有限公司
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Problems solved by technology

[0004] However, when the epidermis and dermis are separated, the epidermal tissue is relatively dense and difficult to digest; after a long period of digestion, the cells are severely damaged, resulting in difficulty in growth and high mortality
Although it is said that reducing the digestion time and increasing the frequency of cell collection can reduce the death rate of cells; however, there is still a large number of cell death, and the operation is cumbersome
Moreover, due to the poor cell activity, the cell adhesion rate is low and the growth rate is slow
[0005] At the same time, more epidermal cells are mixed in the cultured cells, and the purity of the final product is affected; additional steps are required for processing
Further lead to increased cost and increased process complexity

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  • Preparation method of skin fibroblasts

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0031] In describing the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", " The orientation or positional relationship indicated by "outside", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, so as to Specific orientation configurations and operations, therefore, are not to be construed as limitations on the invention.

[0032] refer to figure 1...

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Abstract

The invention discloses a preparation method of skin fibroblasts, and belongs to the technical field of biological cells. The preparation method of the skin fibroblasts comprises the following steps of: S01, specimen treatment: treating a skin specimen, and removing adipose tissues and connective tissues; S02, separation: treating the treated specimen with neutral protease, and removing epidermis to obtain dermis tissues; S03, digestion: digesting the dermis tissues by using pancreatin to obtain tissue fluid; S04, centrifugation: centrifuging the tissue fluid, and removing supernatant to obtain a cell suspension; S05, culture: transferring the cell suspension into a culture dish, adding a culture solution, and culturing; and S06, passage: culturing until the fusion degree is 75-85%, and performing passage culture. The preparation method disclosed by the invention has the advantages of simple steps, low possibility of pollution, a high cell survival rate, a good purification effect and small cell loss.

Description

technical field [0001] The invention relates to the technical field of biological cells, in particular to a preparation method of skin fibroblasts. Background technique [0002] Fibroblasts are the main components of dermal tissue and play an important role in the construction of artificial skin and tissue wound repair; the study on the culture of fibroblasts provides a powerful tool for studying the mechanism of wound healing at the cellular level. [0003] At present, for the culture and preparation of skin fibroblasts, the method adopted is as follows: the obtained specimen tissue is digested, and after the dermal cells are separated, they are centrifugally screened and cultured. [0004] However, when the epidermis and dermis are separated, the epidermal tissue is relatively dense and difficult to digest; after a long period of digestion, the cells are severely damaged, resulting in difficulty in growth and high mortality. Although it is said that reducing the digestion...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0656C12N2509/00C12N2509/10
Inventor 赵辉崔兰云高萧芳孙宪凯赵勇涛
Owner 郑州源创基因科技有限公司
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