Tissue filtering method for extracting cartilage cells

A filtration method and chondrocyte technology, applied in cell dissociation methods, sterilization methods, bone/connective tissue cells, etc., can solve the problems of increasing the risk of cell contamination, reducing the digestion effect, and high production costs, and achieving good contact , Enhanced digestion effect, low cost effect

Pending Publication Date: 2021-06-01
XIANGTAN UNIV
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Problems solved by technology

[0014] Although this device for digestion, filtration and collection of placental tissue cells can also be used to extract, digest and filter chondrocytes, due to the existence of the filter handle, the filter can only be erected above the collector, and the tissues in the filter cannot be better connected with the digestive fluid. Contact for digestion, will reduce the effect of digestion
Moreover, the device is relatively complicated and inconvenient to operate. In the process of collecting and removing the supernatant, it is necessary to hold the filter handle to take out the filter, which will increase the risk of cell contamination. In addition, the production cost of the device is also high

Method used

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  • Tissue filtering method for extracting cartilage cells
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Embodiment Construction

[0037] Specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0038] The present invention provides a tissue filtration method for extracting chondrocytes, the method is carried out in a tissue filtration device, specifically, as figure 1 and figure 2 As shown, the tissue filtering device includes a tubular part 1, a filter screen 2 and a clamping part 3, the filter screen 2 is arranged at one end of the tubular part 1 and covers the opening at one end of the tubular part 1, and the clamping part 3 is arranged on the tubular part 1 On the inner wall of the other end, the clamping part 3 is arranged to be convenient for the user to use corresponding clamps (such as tweezers) to clamp and fix.

[0039] During the process ...

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Abstract

The invention relates to the field of application of medical instruments and discloses a tissue filtering method for extracting cartilage cells, the method is carried out in a tissue filtering device, the tissue filtering device comprises a tubular part (1), a filter screen (2) and a clamping part (3); the filter screen (2) is arranged at one end of the tubular part (1) and covers an opening located at one end of the tubular part (1); the clamping part (3) is arranged on the inner wall of the other end of the tubular part (1), and the clamping part (3) is arranged to be convenient for a user to clamp and fix by using a corresponding clamp. The tissue filtering device applied in the method has the advantages of being low in cost, convenient to operate and not prone to pollution.

Description

technical field [0001] The invention relates to the application field of medical devices, in particular to a tissue filtration method for extracting chondrocytes. Background technique [0002] With the rapid development of my country's transportation and sports and the acceleration of the aging society, the incidence of articular cartilage trauma, strain, and degenerative diseases is increasing year by year. Cartilage tissue is a kind of connective tissue without lymph, blood vessels, and nerves, so the regeneration and repair ability of cartilage is extremely limited, and once the defect is almost impossible to heal. At present, the clinical treatment of cartilage injury mainly depends on drug control and transplantation. Cartilage tissue engineering is a research hotspot in recent years. Autologous chondrocytes are an important cell source for chondrocyte transplantation and the most ideal seed cells for cartilage tissue engineering. [0003] Cartilage tissue is mainly c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12M1/24C12M1/12C12M1/00
CPCC12M23/06C12M33/14C12M47/04C12N5/0655C12N2509/00C12N2509/10
Inventor 吴萍陈慧谭小宁刘仁琴杨春光严建业彭利红李归
Owner XIANGTAN UNIV
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