Open type simplified culture medium for taxus cuspidata

A medium and open technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of undeveloped varieties, declining sales of test-tube seedlings, and difficulty in rooting tissue culture seedlings. To achieve the effect of benefiting the growth of seedlings, reducing manpower and equipment investment, saving costs and inoculation time

Inactive Publication Date: 2013-08-14
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional closed tissue culture has many limitations, such as: there are many kinds of chemicals used in the preparation of culture medium, the dosage is large, the requirements are fine, the price is expensive, the equipment is strict during the tissue culture process, and strict sterilization conditions are required, resulting in power consumption The quantity is large, the rooting method is cumbersome, and some tissue culture seedlings are difficult to root, poor adaptability to transplanting, low survival rate and other problems greatly increase the cost of tissue culture, and even cause the price of test tube seedlings to be several times or even dozens of times higher than ordinary seedlings times the cost, the majority of growers are unable to purchase, and the sales of test-tube seedlings have declined, which seriously limits the expansion and promotion of this technology, resulting in many varieties not being developed and utilized.

Method used

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  • Open type simplified culture medium for taxus cuspidata
  • Open type simplified culture medium for taxus cuspidata
  • Open type simplified culture medium for taxus cuspidata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] According to Table 1 and Table 2, prepare the primary culture medium:

[0028] a. According to the use concentration and standard B of inorganic salts in Table 1 5 Use concentration of other components in the medium to prepare optimized B 5 Basic mother liquor, divided into No. 1, No. 2, ..., No. 7 beakers.

[0029] B, indolebutyric acid IBA and agricultural streptomycin were added in each beaker with optimized B 5 The base stock solution is mixed to the desired concentration.

[0030] c. Take a certain amount of tap water (instead of distilled water) and boil it. Use an electronic balance to take carrageenan and sugar. Pour the carrageenan into the boiling water and boil until the volume reaches 1L. Then pour white sugar into it. After melting, take the carrageenan solution. Packed in No. 1, No. 2, ..., No. 7 beakers. After filling, stir well.

[0031] d. Use an analytical balance to weigh the activated carbon and add it to No. 1, No. 2, ..., and No. 7 beakers. Wh...

Embodiment 2

[0038] Preparation of explants:

[0039] The day before the test, take the semi-lignified 2-3cm stem tip of the current year and wash it with running water for 12-24 hours;

[0040] Operate under the ultra-clean table, wipe the table and hands clean with 70% alcohol, and light the alcohol lamp.

[0041] Disinfect the cleaned semi-lignified stem tips under a sterile ultra-clean bench, disinfect with 75% alcohol for 30-60 seconds, and then use 0.1% mercury chloride (HgCl 2) for 7 to 8 minutes, rinse with sterile water (autoclaved tap water) for 4 to 5 times after disinfection, and then use sterile filter paper (autoclaved filter paper) to dry the surface water stains.

[0042] Burn the scalpel and tweezers on the outer edge of the alcohol lamp, dip in 95% alcohol, and repeat 3 times (for about 3 minutes). On the petri dish, the leaves at the base of 1 cm were cut off, and at the same time, the corroded incisions due to disinfection were removed, and the explants were prepared....

Embodiment 3

[0044] Inoculation and culture of explants

[0045] Primary culture:

[0046] The inoculation was carried out next to the alcohol lamp under the sterile ultra-clean table, and the prepared explants were inoculated into the primary medium of each plastic cup with sterilized tweezers, 3 to 4 explants per cup, and each inoculation One bottle is sealed with a sterile breathable film.

[0047] After inoculation, the inoculation cups were placed in an artificial climate box, and the environmental parameters of each inoculation cup were as follows:

[0048] Culture conditions: temperature 26°C, humidity 85%, light intensity 2000lux, light and dark alternately 12h / 12h.

[0049] After 15 days of primary culture, statistics were made on bud germination and callus induction in each plastic cup (see Table 2 for experimental data.

[0050] Subculture:

[0051] The inoculation was carried out next to the alcohol lamp under the sterile ultra-clean table, and the seedlings with similar gr...

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Abstract

The invention relates to an open type simplified culture medium for taxus cuspidate. The culture medium comprises a primary culture medium and a subculture medium. The primary culture medium is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, active carbon AC and agricultural streptomycin into optimized B5 basic mother liquor. The subculture is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, 6-benzyl amino adenine BA, active carbon AC and agricultural streptomycin into optimized MS basic mother liquor. According to the culture medium provided by the invention, with the adoption of open type simplified tissue culture, the cost and the inoculating time of the culture medium are remarkably saved, the autoclaved sterilization program is cancelled, and the production flow is simplified by replacing autoclaved sterilization and strict enclosed environment operation by a bacteriostatic agent, so that the labor and equipment investment in industrialized seedling production can be reduced, and the fund is saved by 30-50%.

Description

technical field [0001] The invention belongs to the technical field of industrialized seedling culture medium for plant tissue culture, and relates to an open and simplified plant tissue culture medium for Taxus chinensis. Background technique [0002] At present, although the tissue culture of thousands of plants has been successfully achieved, there are not many plant species that can be used for commercial production. Conventional closed tissue culture has many limitations, such as: there are many kinds of chemicals used in the preparation of culture medium, the dosage is large, the requirements are fine, the price is expensive, the equipment is strict during the tissue culture process, and strict sterilization conditions are required, resulting in power consumption The quantity is large, the rooting method is cumbersome, and some tissue culture seedlings are difficult to root, poor adaptability to transplanting, low survival rate and other problems greatly increase the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王淑杰刘丽丽袁丽娜于海业钟香梅李纯牛海微户新宇张蕾姚宁宁隋媛媛赵静
Owner JILIN UNIV
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