Open type simplified culture medium for taxus cuspidata

A medium and open technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of undeveloped varieties, declining sales of test-tube seedlings, and difficulty in rooting tissue culture seedlings. To achieve the effect of benefiting the growth of seedlings, reducing manpower and equipment investment, saving costs and inoculation time
CN103238522AInactive Publication Date: 2013-08-14JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2013-08-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an open type simplified culture medium for taxus cuspidate. The culture medium comprises a primary culture medium and a subculture medium. The primary culture medium is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, active carbon AC and agricultural streptomycin into optimized B5 basic mother liquor. The subculture is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, 6-benzyl amino adenine BA, active carbon AC and agricultural streptomycin into optimized MS basic mother liquor. According to the culture medium provided by the invention, with the adoption of open type simplified tissue culture, the cost and the inoculating time of the culture medium are remarkably saved, the autoclaved sterilization program is cancelled, and the production flow is simplified by replacing autoclaved sterilization and strict enclosed environment operation by a bacteriostatic agent, so that the labor and equipment investment in industrialized seedling production can be reduced, and the fund is saved by 30-50%.
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Description

technical field

[0001] The invention belongs to the technical field of industrialized seedling culture medium for plant tissue culture, and relates to an open and simplified plant tissue culture medium for Taxus chinensis. Background technique

[0002] At present, although the tissue culture of thousands of plants has been successfully achieved, there are not many plant species that can be used for commercial production. Conventional closed tissue culture has many limitations, such as: there are many kinds of chemicals used in the preparation of culture medium, the dosage is large, the requirements are fine, the price is expensive, the equipment is strict during the tissue culture process, and strict sterilization conditions are required, resulting in power consumption The quantity is large, the rooting method is cumbersome, and some tissue culture seedlings are difficult to root, poor adaptability to transplanting, low survival rate and other problems greatly increase the c...

Claims

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