Special thermophilic bacteria for catalytic production of alpha-ketoglutaric acid and catalytic production method

A technology of ketoglutaric acid and high-temperature bacteria, which is applied in the field of enzyme engineering, can solve the problems of low temperature in the catalytic production process and easy occurrence of bacterial contamination, achieve large industrial application value, be suitable for industrial production, and increase production.

Pending Publication Date: 2022-06-24
DAZIRAN BIOLOGICAL GRP CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, microbial fermentation companies have certain technical precautions in preventing bacterial contamination, but companies engaged in biocatalysis often focus on chemical methods, so bacterial contamination often becomes their technical bottleneck
[0004] At present, the production process of converting glutamic acid into α-ketoglutaric acid by enzymatic catalysis generally has the problems of low temperature in the catalytic production process and prone to bacterial contamination. Microbial strains and microbial strains produce glutamic acid oxidase (LGOX), and the ability of glutamate oxidase (LGOX) to catalyze the formation of α-ketoglutarate from glutamic acid is the key to the catalytic production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Utilize high temperature actinomycetes Streptomyces sp.TC2303 to catalyze the method for producing α-ketoglutaric acid, comprising the following steps:

[0028] S1. Preparation of fermentation broth:

[0029] Take 1 single colony of the strain Streptomyces sp.TC2303 obtained by streaking on the plate and put it in the shake flask fermentation medium, and culture the shake flask at 45°C for 24 hours. Use a 500mL conical flask with a liquid filling volume of 100mL, and centrifuge to obtain a supernatant. , which is the fermentation broth (enzyme liquid).

[0030] The above shake flask fermentation medium formula is as follows: glucose 20g, yeast powder 5g, ammonium nitrate 3g, potassium dihydrogen phosphate 1g, calcium chloride 0.1g, magnesium sulfate 0.3g, ferrous sulfate 0.01g, corn steep liquor 1g, with hydrochloric acid or Adjust the pH to about 7.5 with sodium hydroxide, add water to 1L, and sterilize at 110°C for 10min.

[0031] S2, prepare the initial reaction so...

Embodiment 2

[0035] Utilize high temperature actinomycetes Streptomyces sp.TC2303 to catalyze the method for producing α-ketoglutaric acid, comprising the following steps:

[0036] S1. Preparation of fermentation broth:

[0037] Take 1 single colony of the strain Streptomyces sp.TC2303 obtained by streaking on the plate and put it in the shake flask fermentation medium, and culture the shake flask at 45°C for 24 hours. Use a 500mL conical flask with a liquid filling volume of 100mL, and centrifuge to obtain a supernatant. , which is the fermentation broth (enzyme liquid).

[0038] The above-mentioned shake flask fermentation medium formula is as follows: glucose 18g, yeast powder 4g, ammonium nitrate 3g, potassium dihydrogen phosphate 1g, calcium chloride 0.05g, magnesium sulfate 0.2g, ferrous sulfate 0.01g, corn steep liquor 0.8g, with hydrochloric acid Or adjust the pH to about 7.5 with sodium hydroxide, add water to 1L, and sterilize at 110°C for 10min.

[0039] S2, prepare the initia...

Embodiment 3

[0043] Utilize high temperature actinomycetes Streptomyces sp.TC2303 to catalyze the method for producing α-ketoglutaric acid, comprising the following steps:

[0044] S1. Preparation of fermentation broth:

[0045] Take 1 single colony of the strain Streptomyces sp.TC2303 obtained by streaking on the plate and put it in the shake flask fermentation medium, and culture the shake flask at 45°C for 24 hours. Use a 500mL conical flask with a liquid filling volume of 100mL, and centrifuge to obtain a supernatant. , which is the fermentation broth (enzyme liquid).

[0046] The above-mentioned shake flask fermentation medium formula is as follows: glucose 23g, yeast powder 6g, ammonium nitrate 4g, potassium dihydrogen phosphate 1.3g, calcium chloride 0.15g, magnesium sulfate 0.3g, ferrous sulfate 0.01g, corn steep liquor 1.5g, with Adjust pH to about 7.5 with hydrochloric acid or sodium hydroxide, add water to 1L, and sterilize at 110°C for 10min.

[0047] S2, prepare the initial ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a special thermophilic bacterium for catalytic production of alpha-ketoglutaric acid and a catalytic production method. The strain is Streptomyces sp.TC2303 and is preserved in the China Center for Type Culture Collection on December 27, 2021, the preservation date is December 27, 2021, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20211682. The culture and fermentation conditions of the strain Streptomyces sp.TC2303 provided by the invention are both about 45 DEG C, the fermentation time is only 24 hours, and the fermentation time is short; according to the present invention, the fermentation broth can be used to catalyze L-glutamic acid at the temperature of 45-56 DEG C to produce alpha-ketoglutaric acid, the conversion process temperature is more than 45 DEG C, the catalysis temperature is far higher than the prior art, the total reaction time is only 18 h, the catalysis process is not easily subjected to bacterial contamination, and the method has special advantages for enterprises with simple and crude equipment and easy bacterial contamination.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to a special high-temperature bacteria for catalyzing the production of α-ketoglutarate and a catalyzing production method. Background technique [0002] Alpha-ketoglutaric acid has a wide range of applications in food, chemical and other fields. At present, the methods for industrial production of α-ketoglutaric acid are all chemical synthesis methods. Because the production process of this method involves some toxic chemicals, the application of its products in the food field has certain difficulties. [0003] my country is a big country in the production of glutamic acid, and the current production capacity of glutamic acid is excessive. There are some reports at home and abroad about the method of converting glutamic acid into α-ketoglutarate by enzymatic catalysis. In the biocatalytic process, microbial strains are generally first cultivated. The proces...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P7/50C12R1/465
CPCC12N1/20C12N1/02C12P7/50
Inventor 徐恒德于有良李建波陈献忠刘家印贾庆福许菲于召涛
Owner DAZIRAN BIOLOGICAL GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap