Special thermophilic bacteria for catalytic production of alpha-ketoglutaric acid and catalytic production method

Abstract

The invention discloses a special thermophilic bacterium for catalytic production of alpha-ketoglutaric acid and a catalytic production method. The strain is Streptomyces sp.TC2303 and is preserved in the China Center for Type Culture Collection on December 27, 2021, the preservation date is December 27, 2021, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20211682. The culture and fermentation conditions of the strain Streptomyces sp.TC2303 provided by the invention are both about 45 DEG C, the fermentation time is only 24 hours, and the fermentation time is short; according to the present invention, the fermentation broth can be used to catalyze L-glutamic acid at the temperature of 45-56 DEG C to produce alpha-ketoglutaric acid, the conversion process temperature is more than 45 DEG C, the catalysis temperature is far higher than the prior art, the total reaction time is only 18 h, the catalysis process is not easily subjected to bacterial contamination, and the method has special advantages for enterprises with simple and crude equipment and easy bacterial contamination.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to a special high-temperature bacteria for catalyzing the production of α-ketoglutarate and a catalyzing production method. Background technique [0002] Alpha-ketoglutaric acid has a wide range of applications in food, chemical and other fields. At present, the methods for industrial production of α-ketoglutaric acid are all chemical synthesis methods. Because the production process of this method involves some toxic chemicals, the application of its products in the food field has certain difficulties. [0003] my country is a big country in the production of glutamic acid, and the current production capacity of glutamic acid is excessive. There are some reports at home and abroad about the method of converting glutamic acid into α-ketoglutarate by enzymatic catalysis. In the biocatalytic process, microbial strains are generally first cultivated. The proces...

AI Technical Summary

Problems solved by technology

Generally, microbial fermentation companies have certain technical precautions in preventing bacterial contamination, but companies engaged in biocatalysis often focus on chemical methods, so bacterial contamination often becomes their technical bottleneck

[0004] At present, the production process of converting glutamic acid into α-ketoglutaric acid by enzymatic catalysis generally has the problems of low temperature in the catalytic production process and prone to bacterial contamination. Microbial strains and microbial strains produce glutamic acid oxidase (LGOX), and the ability of glutamate oxidase (LGOX) to catalyze the formation of α-ketoglutarate from glutamic acid is the key to the catalytic production process