Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof

Abstract

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

Description

technical field [0001] The invention relates to the technical field of application of agricultural microorganisms, in particular, the invention relates to an antagonistic bacteria which cooperates with AMF to resist disease and promote growth and its application technical field in the prevention and treatment of vegetable soil-borne diseases. Background technique [0002] In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable planting, and as the country and the autonomous region attach importance to the vegetable basket project, the development speed and scale of facility agriculture have increased and expanded year by year, but due to facility agriculture The reality that pests and diseases are prone to occur and difficult to control, therefore, higher requirements are put forward for the prevention and control of pests and diseases, especially vegetable diseases in facility agriculture are par...

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Problems solved by technology

[0002] In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable planting, and as the country and the autonomous region attach importance to the vegetable basket project, the development speed and scale of facility agriculture have increased and expanded year by year, but due to facility agriculture The reality that pests and diseases are prone to occur and difficult to control, therefore, higher requirements are put forward for the prevention and control of pests and diseases, especially vegetable diseases in facility agriculture are particularly serious, and soil-borne diseases are one of the main goals of prevention and control; soil-borne diseases Diseases refer to diseases caused by pathogens living in the soil or pathogens in the residues of diseased plants in the soil, which are invaded from the roots or stems of crops; soil-borne diseases of vegetables in facility agriculture mainly include vegetable root-knot nematode disease and melon wilt , Verticillium wilt, dry-off at seedling stage, damping-off disease, etc.; Due to the limitation of geographical conditions, it is difficult to rotate crops and stubble, and the continuous cropping cycle is long, and conditions such as temperature and humidity are conducive to the occurrence of various diseases. It is very difficult to prevent and control soil-borne diseases. In recent years, its high incidence rate and great harm have become the main obstacle factors affecting the production efficiency of facility agriculture vegetables, seriously restricting the development of vegetable industry; AM fungi arbuscular mycorrhizal fungi ( Arbuscular Mycorrhizal Fungi , referred to as AMF, AM fungi, formerly known as VA bacteria) are ubiquitous in soils around the world, and their hosts include bryophytes, ferns, gymnosperms and angiosperms; according to statistics, 90% of vascular plants and agricultural ecology Most crops in the system can form arbuscular mycorrhiza; the symbiosis of arbuscular mycorrhizal fungi and plants can increase the absorption area of ​​plant roots, increase the absorption of mineral nutrients, especially phosphorus, and thus promote the growth of plant shoots and roots. At the same time, it can also improve the stress resistance of plants; there are many reports and studies on the effect and mechanism of the interaction between AM fungi and PGPR on the growth and disease control of crops by a single AMF, but it can cooperate with AM fungi to resist disease The growth-promoting antagonistic bacteria have not been reported publicly

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