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Preparation method of spore preparation of bacillus coagulans

A technology of Bacillus coagulans and spores, which is applied in the field of preparation of spore preparations, can solve problems such as insufficient acid production, low spore rate, and complex inoculation operation on liquid slopes, and solve solid fermentation pollution, concentration of spore formation stages, and shorten the preparation cycle Effect

Inactive Publication Date: 2014-12-10
HANGZHOU BIOCOM BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The invention proposes a method for preparing a spore preparation of Bacillus coagulans, which solves the problems of high energy consumption, large environmental pollution and high cost in the prior art
[0014] The present invention further proposes a preparation method of a spore preparation of Bacillus coagulans, which solves the problems of low product concentration, low spore rate, insufficient acid production and contamination by miscellaneous bacteria in the prior art
[0015] The present invention further proposes a preparation method of a spore preparation of Bacillus coagulans, which solves the problems of complex inoculation operation, long period and easy pollution in the prior art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of a spore preparation of bacillus coagulans, comprising:

[0040] 1. Bacillus coagulans primary tube slant seeds

[0041] The original bacteria were inoculated on the primary slant medium, and cultured at 30°C-45°C for 20-32h.

[0042] The primary slant medium is a mixed solution (g / L) prepared according to the following ratio: yeast extract 5-15g / L peptone 5-15g / L glucose 0.5-5g / L sodium chloride 5-10g / L phosphoric acid Dipotassium hydrogen 1~5g / L manganese sulfate 0.1~2g / LpH7.0~7.2.

[0043] 2. Bacillus coagulans secondary liquid seeds

[0044] Inoculate the single colony of the primary Bacillus coagulans in step 1 into the secondary culture medium, the liquid volume is 50-100mL / 500mL, and cultivate it under the conditions of 30°C-45°C and 160-300rpm / min for 20-32h to obtain the secondary culture medium Bacillus coagulans seed solution.

[0045] The secondary liquid medium is a mixed solution (g / L) prepared according to the following ratio: g...

Embodiment 2

[0053] A preparation method of a spore preparation of bacillus coagulans, comprising:

[0054] 1. Bacillus coagulans primary tube slant seeds

[0055] The original bacteria were inoculated on the primary slant medium, and cultured at 30°C-45°C for 20-32h.

[0056] The primary slant medium is a mixed solution (g / L) prepared according to the following ratio: yeast extract 5-15g / L peptone 5-15g / L glucose 0.5-5g / L sodium chloride 5-10g / L phosphoric acid Dipotassium Hydrogen 1~5g / L Manganese Sulfate 0.1~2g / LpH7.0~7.2

[0057] 2. Bacillus coagulans secondary liquid seeds

[0058] Inoculate the single colony of the primary Bacillus coagulans in step 1 into the secondary culture medium, the liquid volume is 50-100mL / 500mL, and cultivate it under the conditions of 30°C-45°C and 160-300rpm / min for 20-32h to obtain the secondary culture medium Bacillus coagulans seed solution.

[0059] The secondary liquid medium is a mixed solution (g / L) prepared according to the following ratio: gl...

Embodiment 3

[0067] A preparation method of a spore preparation of bacillus coagulans, comprising:

[0068] 1. Bacillus coagulans primary tube slant seeds

[0069] The original bacteria were inoculated on the primary slant medium, and cultured at 30°C-45°C for 20-32h.

[0070] The primary slant medium is a mixed solution (g / L) prepared according to the following ratio: yeast extract 5-15g / L peptone 5-15g / L glucose 0.5-5g / L sodium chloride 5-10g / L phosphoric acid Dipotassium Hydrogen 1~5g / L Manganese Sulfate 0.1~2g / L, Agar 15~20g / L, pH7.0~7.2

[0071] 2. Secondary solid seeds of Bacillus coagulans

[0072]Inoculate the single colonies of the primary Bacillus coagulans in batches in the secondary eggplant-type culture flasks in step 1, culture them at 30°C-45°C for 36-48 hours, and store them at 4°C for 24 hours.

[0073] The medium in the described secondary eggplant type culture flask is the same as that in step 1.

[0074] 3. Solid fermentation culture of Bacillus coagulans

[0075] ...

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PUM

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Abstract

The invention provides a preparation method of a spore preparation of bacillus coagulans. The preparation method of the spore preparation of bacillus coagulans comprises the following steps of: (a) slope thallus activation; (b) seeding tank cultivation of the bacillus coagulans; and (c) solid fermentation of the bacillus coagulans: placing the culture medium in the step (b) in a solid fermentation medium based on the weight ratio of 1%-10%, and fermenting for 48-72hours in a semi-closed manner. By using a semi-closed solid fermentation method, materials are in the semi-closed state in the process of fermentation and contact with a small amount of air, the bacillus coagulans can be propagated greatly by fully utilizing residual oxygen in the growing period, oxygen is greatly reduced in the later growing period, spore can grow rapidly, and more L-lactic acid can be metabolized, so that the problem that produced acid is insufficient in the fermentation process is solved, the growth of infectious microbe is inhibited through oxygen consumption, and the phenomenon of solid fermentation pollution is avoided.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of a spore preparation of Bacillus coagulans. Background technique [0002] In recent years, the frequent incidents of "lean meat powder", "turkey fish" and "red duck eggs" have sounded the national alarm on food safety, and have also aroused great attention from the party, the government and the general public. Traditional animal husbandry develops in pursuit of high feed remuneration, high growth rate, low cost to improve economic efficiency, and at the cost of sacrificing the environment and consuming resources. However, my country's animal husbandry will still account for a large proportion of the agricultural economy in a certain historical period. Therefore, the development of efficient and pollution-free breeding production technology is an inevitable way for the development of my country's animal husbandry. Pollution-free feed additive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 王云龙吴勃
Owner HANGZHOU BIOCOM BIOLOGICAL TECH
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