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Strain with high destruxins yield and application thereof

A technology of metarhizine and bacteria strains, applied in the direction of fungi, biochemical equipment and methods, microorganisms, etc., to achieve good stability, increase production, and reduce production costs

Inactive Publication Date: 2009-10-21
GUANGDONG XINJINGXIANG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, high-yielding strains of metarhizine A have not been reported

Method used

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  • Strain with high destruxins yield and application thereof
  • Strain with high destruxins yield and application thereof
  • Strain with high destruxins yield and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] By collecting the carcasses of a susceptible gill beetle (Exolontha sp.) in Guangzhou (as attached image 3 As shown), using conventional general techniques to isolate and purify strains, carry out steps such as biological characteristics and production traits comparison, and strain determination, etc., after repeated screening, a strain of Metarhizium anisopliae var. anisopliae with high metarhizin production was obtained, numbered MaQ10 .

[0023] Preparation of spore suspension: pick spores from the inclined surface, insert them into sterilized 0.05% Tween-80 solution, measure the spore concentration with a hemocytometer, and adjust to 10 7 Spores / mL.

[0024] Seed culture: Inoculate 2mL of spore suspension into 98mL of seed medium, shake the flask at 180r / min and 26°C for 3 days to form a seed solution.

[0025] Seed medium composition: Czapek-Dox with 0.5% peptone: Peptone 5g, NaNO 3 3g,K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, suc...

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Abstract

The invention disclose a strain with high destruxins yield and application thereof. Classification status of the strain MaQ10 is as follows: asexual generation eumycophyta, mitospore eumycophyta, hyphomycetes, moniliales, hyphomycetaceae and metarrhizium; the strain is conserved at China Center for Type Culture Collection, conservation date is October 27, 2008, the conservation number is CCTCC M 208173. The metarrhizium strain has the advantages of high yield of destruxins A and destruxins B, stable strain yield, in particular good stability of destruxins yield before the 10th generation. The strain can provide technical base for increasing the actual yield and reducing production cost of the destruxins.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a high-yield Metarhizium anisopliae strain and its application. Background technique [0002] Metarhizium anisopliae is a cyclic depsipeptide antibiotic produced by entomopathogenic fungi such as Metarhizium anisopliae. Metarhizine has significant insecticidal effect, and has a brand-new mechanism of action different from existing insecticides. It mainly acts on the hemolymph immune system of insects, causing them to lose their immune defense capabilities. Metarhizin is easy to degrade, has no residue, and has low toxicity. The development and utilization of metarhizine is of great economic significance. However, the yield of metarhizin in general fungal strains is very low, and the cost of producing metarhizin is very expensive. [0003] In Europe, Loutelier et al. (1996) reported that the Ma23 strain of Metarhizium anisopliae had a higher yield of m...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P21/04C12R1/645
Inventor 胡琼波任顺祥
Owner GUANGDONG XINJINGXIANG BIOLOGICAL ENG CO LTD
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