Culture medium for production of metarhizium anisopliae

Abstract

The invention relates to a new formula of a culture medium used in culture of metarhizium anisopliae which is mainly used for transforming W-oxides to mildew oxides during the biological fermentation process of sterides, and the new formula of the culture medium belongs to the technical field of fermentation of industrial fungi. The new formula of the culture medium is as follows: each 1000ml of the culture medium contains 2.0%-4.5% of glucose by weight, 0.4%-0.7% of peptone by weight, 0.03%-0.07% of magnesium sulfate by weight, 0.2%-0.6% rapeseed oil cake by weight, 0.1%-0.3% prinsepia utilis royle powder by weight, 0.1%-0.3% cotton seed meal by weight and the balance of water. The metarhizium anisopliae cultured by the culture medium is rapid and uniform in growth, and thalli are less prone to balling generally; and in addition, most of raw materials adopted by the culture medium are waste materials from industrial production, the sources are wide, the yield is great, the metarhizium anisopliae can be supplied all the year round, the production cost is low, and the characteristics of low carbon and circulatory production are simultaneously reflected.

Description

technical field [0001] The invention relates to a new formula of culture medium used in the cultivation of Metarhizium anisopliae. The Metarhizium anisopliae is mainly used in the process of steroidal biological fermentation to convert Wordler's oxides into mold oxides, which belongs to industrial fungal fermentation technology field. Background technique [0002] Steroids are a class of compounds containing cyclopentane polyhydrophenanthrene nuclei, which are widely used in medicine. During the production of steroids, the hydroxylation reaction is the most important reaction in the microbial transformation of steroids. Since microorganisms can carry out hydroxylation reactions at any position of steroids, and chemical methods are difficult to introduce at other positions except for the introduction of hydroxyl groups at C17, so fungi, such as Metarhizium anisopliae, are used to catalyze the conversion of steroids It is a relatively advanced and feasible production process...

AI Technical Summary

Problems solved by technology

[0003] However, through new search and retrieval, there are few technical documents about the culture medium of Metarhizium anisopliae, especially the culture medium formula for the industrial fungus culture of Metarhizium anisopliae produced by steroid fermentation has not yet been reported.

The culture medium formula of the reported Metarhizium anisopliae in the literature is solid medium mostly, such as PDA medium; The medium of its liquid fermentation adopts to provide trace elements with various chemical raw materials, with glucose, sucrose, potato starch, lactose, Maltose, D-fructose, D-sorbose, mannitol, etc. are used as carbon sources, and peanut cake powder, bean cake powder, wheat bran, fish meal, KNO, etc. are used as nitrogen sources. Cultivate Metarhizium anisopliae as a pathogenic fungus for the biological control of agricultural and forestry pests, and it is not suitable for converting Word's oxides into mold oxides in the process of steroidal biofermentation

Images