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Preparation for killing ostrinia nubilalis metarhizium anisopliae and application of preparation

A technology for Metarhizium anisopliae and corn borer, which is applied in the application, biocide, insecticide and other directions, can solve the problems such as the field control effect is greatly affected by ultraviolet radiation, the wet dispersion effect is poor, and there is no large-scale application, and the invention is suitable for Large-scale production and large-scale promotion and use, outstanding effect, good wetting and dispersing characteristics

Inactive Publication Date: 2015-05-13
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the activity of fungal spores is affected by surfactants, the amount of surfactants is often reduced in the preparation, resulting in poor wetting and dispersing effects; at the same time, the storage period of the product is still short, rarely exceeding 12 months; in addition, the field control effect is affected by ultraviolet radiation. The impact is great, so although the insecticidal fungus wettable powder has been registered, it has not been widely used

Method used

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  • Preparation for killing ostrinia nubilalis metarhizium anisopliae and application of preparation
  • Preparation for killing ostrinia nubilalis metarhizium anisopliae and application of preparation
  • Preparation for killing ostrinia nubilalis metarhizium anisopliae and application of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The virulence, sporulation characteristics and heat resistance characteristics of CQMa421 strain after being inoculated with corn borer and domesticated

[0026]Inoculate the CQMa421 strain on a 1 / 4 SDA plate, incubate at 28°C for 15 days, collect mature conidia, disperse them with 0.05% (W / V) Tween-80, and prepare a final concentration of 1×10 7 spores / ml suspension. The 3-4 instar corn borer larvae were inoculated by the spot method, and each test worm was dripped with 2 μl of the drug with a micro-spotter, and 100 larvae were inoculated. After inoculation, they were raised at room temperature 28°C and humidity 50-70%. Select corn borer worms that are the first to die and put them in a 28°C incubator for 3-5 days of moisturizing culture to make fresh spores grow on the surface of the insects; collect spores and inoculate corn borer 10-14 times for domestication. Under sterile conditions, use an inoculation loop to pick up fresh spores after acclimatization...

Embodiment 2

[0033] Example 2 Protective effect of nanometer graphite powder on Metarhizium anisopliae

[0034] According to Table 2, the pure sporopollen of Metarhizium anisopliae CQMa421 was mixed with nano-graphite powder, and then 45% kaolin, 5% CMC and different contents of Tween-80 and HEL-40 were added and mixed evenly.

[0035] UV protection: disperse the preparation sample with water to make a concentration of 1×10 7 After spores / mL of spore suspension, take 100μl of spore suspension and spread it on 1 / 4 SDA plate, mix well with glass and put it in 290-310nm ultraviolet lamp (978mW / m 2 ) under vertical irradiation for 5 hours, and then cultured in a 28°C incubator for 24 hours to measure the germination rate (the method is the same as above), and repeat 3 times. The data were processed and analyzed with SPSS software.

[0036] Storage experiment: Store the preparation sample in a sealed container at 28°C for 2 months, and disperse it with water to make a concentration of 1×10 ...

Embodiment 3

[0041] Example 3 Preparation of wettable powder of Metarhizium anisopliae CQMa421 and its wettability, storage period and control effect.

[0042] According to Table 3, after mixing the pure sporopollen of Metarhizium anisopliae CQMa421 with nano-graphite powder, different contents of kaolin, CMC, Tween-80 and HEL-40 were added.

[0043] Determination of wettability of wetting and dispersing agent: Take 100mL of standard water and pour it into a 250mL beaker. Place the beaker in a constant temperature water bath at 25°C±1°C, so that the liquid level is flush with the water bath level. When the hard water reaches 25°C±1°C, accurately weigh 5.0g of the sample to be tested and place it on a watch glass. Do not disturb the liquid level excessively. Immediately record the time with a stopwatch when adding the sample until the sample is completely wetted (the fine powder film remaining on the liquid surface can be ignored). Note down the wetting time (accurate to the second). ...

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Abstract

The invention discloses an application of a metarhizium anisopliae CQMa421 strain in preparation of agents for controlling ostrinia nubilalis. The metarhizium anisopliae CQMa421 strain can infect the ostrinia nubilalis, the fatality rate can be 66.5 percent; if a metarhizium anisopliae CQMa421 strain prepared by adopting a toxicity enhancing method is adopted, the fatality rate is up to 100%, LT50 is shortened by 60 percent, and the effect is very outstanding. According to the metarhizium anisopliae preparation disclosed by the invention, the various components are effectively matched with one another, a synergistic effect is achieved, and when the metarhizium anisopliae preparation is blended with water to spray, the field efficacy of ostrinia nubilalis is over 80 percent. The preparation has high ultraviolet resistance and wetting and dispersing characteristics, the biological activity of the preparation in long-term storage is maintained, and the storage time can be 12 months.

Description

technical field [0001] The invention relates to a new application of a strain of Metarhizium anisopliae, in particular to a preparation of Metarhizium anisopliae for killing corn borers and an application thereof, belonging to the field of biological pesticides. Background technique [0002] Corn borer is the main pest of corn in my country, which seriously affects the yield and quality of corn, and can cause 10-30% yield loss every year. At present, chemical pesticides are mainly used to control corn borer, which not only directly damages the environment and seriously weakens the natural control ability of the field, but also makes it difficult to control corn borer due to the emergence of resistance to corn borer. Therefore, the development of safe and sustainable biopesticides to replace chemical pesticides is of great significance to protect the environmental safety and biodiversity of corn planting areas. [0003] Entomopathogenic fungi are the most important group of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01N25/14A01P7/04C12R1/645
CPCA01N25/14A01N63/30C12N1/14C12N1/36A01N2300/00
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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