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Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof

A kind of technology of Metarhizium anisopliae and extract, applied in the direction of botany equipment and methods, insecticides, biocides, etc.

Inactive Publication Date: 2014-06-04
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, furbendron is a benzoylurea insecticide. Although it is an important high-efficiency and low-toxicity insecticide, there is still a certain distance between this compound pesticide and the green pesticide.

Method used

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  • Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof
  • Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof
  • Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The tea leaf extract is prepared according to the following method:

[0029] (1) Mixed solvent extraction: After drying the tea leaves, crush them with a pulverizer, pass through a 200-mesh sieve, add methanol and acetone mixed extract (methanol and acetone volume ratio 8:1), and heat to 75 ℃ reflux extraction 3 times, each time add methanol and acetone mixed extract volume (ml): the quality of the sieve (g)=8:1, each extraction time is 3 hours, after merging each extract, Use a rotary evaporator to carry out vacuum distillation to remove methanol and acetone until the methanol and acetone are all volatilized to obtain extracts of methanol and acetone; volatilized methanol and acetone are recycled;

[0030] (2) Water extraction: add water to the extract of the methanol and acetone extract described in step (1) and mix evenly to obtain an aqueous solution of the methanol and acetone extract, and the quality of the added water is equivalent to 3 times the mass of the extr...

Embodiment 2

[0049] In this example, the insecticidal efficacy of the compound insecticide compounded with tea leaf extract and Metarhizium anisopliae is shown in Table 4-6.

[0050] (1) Poisonous effect on Spodoptera litura

[0051] After diluting the insecticide of Example 2 with water in different multiples (1000 times, 1500 times, 2000 times), spray it on the surface of cabbage leaves, then raise live Spodoptera litura on the cabbage leaves to carry out the insecticidal efficacy test, repeat Three times, and set up 3 groups of control groups in addition: Control group 1: do not spray the compound insecticide of embodiment 2; Control group 2: spray the oleifera extract insecticide of 1000 times dilution Powder, sorbitol polyoxyethylene ether, carboxymethyl cellulose, and bentonite were mixed according to mass percentage 60: 20: 10: 10); control group 3: spraying 1000 times diluted insecticide of Metarhizium anisopliae (specific formula: Metarhizium anisopliae ( Metarhizium anisopliae...

Embodiment 3

[0064] In this example, the insecticidal efficacy of the compound insecticide compounded with tea leaf extract and Metarhizium anisopliae is shown in Table 7-9.

[0065] (1) Poisonous effect on Spodoptera litura

[0066] Dilute the compound insecticide of Example 3 with water in different times (1000 times, 1500 times, 2000 times), spray it on the surface of cabbage leaves, and then raise live Spodoptera litura on cabbage leaves for insecticidal efficacy test , repeated three times, and set up 3 groups of control groups in addition: control group 1: do not spray the compound pesticide of embodiment 3; Control group 2: spray the oleifera extract pesticide diluted 1000 times (specific formula: Extract powder, nonylphenol polyoxyethylene ether, dispersant Z, and white carbon black were mixed according to the mass percentage of 60: 5: 20: 15); control group 3: spraying 1000 times diluted scarab metarhizium anisopliae insecticide ( Specific formula: Metarhizium anisopliae ( Meta...

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Abstract

The invention relates to a sasanqua leaf extract and Metarhizium anisopliae complex insecticide and a preparation method thereof. The complex insecticide is prepared from the following raw materials in mass percent: 30-60% of a sasanqua leaf extract, 10-30% of Metarhizium anisopliae, 5-20% of a surface active agent, 10-20% of an assistant and 5-15% of filler. The invention also comprises a preparation method of the sasanqua leaf extract and Metarhizium anisopliae complex insecticide. The sasanqua leaf extract and Metarhizium anisopliae complex insecticide has the characteristics of broad insecticidal spectrum, high activity, stable effect, quick insecticidal effect, strong pesticide resistance, long residual life, little environment influence, convenience in use and the like; according to the invention, the additional value of sasanqua is improved, and the large-scale application of green muscardine fungus is promoted.

Description

technical field [0001] The invention relates to a compound insecticide of a botanical insecticide and a microbial insecticide and a preparation method thereof, in particular to a compound insecticide of tea leaf extract and Metarhizium anisopliae and a preparation method thereof. Background technique [0002] At present, on a global scale, the loss of agricultural products due to agricultural pests reaches 15-25% every year. Large-scale use of chemical pesticides is currently the main strategy for controlling pests. However, the insecticidal specificity of chemical pesticides is poor, the residual effect period in the field is long, and it is easy to induce pests to develop resistance to them, especially the pollution of agricultural products and the environment caused by residual chemical pesticides, leading to miscarriage of women, baby deformities and various human cancers and other diseases, so with the enhancement of human awareness of environmental protection, high-ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P7/04A01N63/04
CPCY02A50/30
Inventor 毛绍名章怀云刘高强
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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