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Metarhizium flavoviride bacterial strain and application thereof

A technology of Metarhizium anisopliae and strains, applied in the direction of fungi, microorganisms, and methods based on microorganisms, can solve the problems of lack of high insecticidal activity and good production characteristics, and achieve good production characteristics, strong sporulation ability, and rapid growth Effect

Active Publication Date: 2012-07-25
成都中农劲威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insecticidal fungi have strong vertical and horizontal diffusion capabilities, are easy to form epidemics, and are helpful for the continuous control of pests. They are especially beneficial for the control of migratory pests rice planthopper, but there is no relevant research on the use of fungi to control rice planthopper. reported that one of the main reasons is the lack of fungal strains with high insecticidal activity and good production traits

Method used

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  • Metarhizium flavoviride bacterial strain and application thereof
  • Metarhizium flavoviride bacterial strain and application thereof
  • Metarhizium flavoviride bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Isolation of bacterial strains

[0018] Disinfect the surface of the collected naturally infected rice planthopper worms with 70% alcohol, put them in a 28°C incubator and culture them for 3-5 days, so that fresh spores grow on the surface of the insects; under sterile conditions, inoculate Pick a small amount of fresh spores and streak them on a PDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 28°C incubator for cultivation; after 3-4 days, pick out typical and For colonies free of miscellaneous bacteria, use a transfer needle to pick up a small piece of medium with hyphae at the edge of the colony, transfer it to the center of the culture medium on the slant of the test tube, and culture at a constant temperature of 28°C for 15 days.

Embodiment 2

[0019] Identification of embodiment 2 strains

[0020] (1) Morphological identification

[0021] The isolated CQMf1072 strain was inoculated on a PDA plate and cultured at 28°C to observe the morphological characteristics of the colony, the sporulation structure and the shape and size of the spores. The results showed that the colony was white cotton flocculent at the initial stage of culture on PDA medium, and olive green during sporulation. The hyphae are separated and branched, transparent, with a diameter of 1.4-1.8 μm; the conidiophores are solitary or aggregated or closely arranged, showing broom-like branches, with a diameter of about 2.2 μm, and the ends produce bottle-shaped stalks, 10.2- 16.1 × 1.5-2.5 μm; chained conidia are continuously formed from the end of the bottle stem, conidia are unicellular, oblong, and the size is 5.2-7.1×2-3.2 μm ( figure 1 shown). From the above characteristics, the strain CQMf1072 should be Metarhizium anisopliae microsporum v...

Embodiment 3

[0025] Example 3 Determination of Indoor Insecticidal Activity of CQMf1072 to Rice Leaf Roller

[0026] Collect the mature spores of CQMf1072 cultured on the PDA plate, disperse them with 0.05% (W / V) Tween-80, and make a final concentration of 1×10 8 spores / ml suspension. The rice planthoppers were transferred into 500ml triangular flasks with wheat seedlings, 30-50 rice planthoppers per bottle, sprayed with a spray tower (POTTER, BURKARD company), and inoculated with 50 μl. In 50-70% of the raw side chambers, 5 replicates were set up, and clear water was used as a blank control. Record the number of dead insects, calculate the survival rate, and obtain the fungal LT by statistical analysis of DPS2000 software 50 (when lethal) and LT 90 (lethal time).

[0027] From image 3 It can be seen that Metarhizium anisopliae CQMf1072 strain has obvious insecticidal activity to rice planthopper. At the peak of death 5-7 days after inoculation, the larvae of rice planthoppers bec...

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Abstract

The invention provides a metarhizium flavoviride bacterial strain (Metarhizium flavoviride.minus) CQMf1072 with the preservation number of CGMCC NO.4717 and the ITS1-5.8S-ITS2 sequence of SEQ ID NO.1. The metarhizium flavoviride bacterial strain CQMf1072 which is directly separated and screened from naturally infected rice planthopper has strong sporulation capability, and the sporulation rate can be up to 2.5%; the metarhizium flavoviride bacterial strain has good production trait and is rapid to grow; meanwhile, the metarhizium flavoviride bacterial strain has high insecticidal activity on the rice planthopper and has a good insecticidal effect; the LT50 (lethal time) and LT90 (lethal time) of the larva of the rice planthopper are respectively 5.8 days and 8.7 days. By using an insecticidal fungal preparation which is manufactured by utilizing the bacterial strain, the population quantity of the rice planthopper is effectively controlled.

Description

technical field [0001] The invention relates to a new microbial strain, in particular to an insecticidal Metarhizium anisopliae strain and application thereof, belonging to the field of biological pesticides. technical background [0002] Rice planthopper is the main migratory pest of rice. It is widely distributed in the main rice areas of the country and seriously threatens my country's grain production. At present, broad-spectrum chemical pesticides are mainly used to control rice planthoppers, which not only directly affects human health and the safety of aquaculture industry, but also kills natural enemies of rice planthoppers and destroys the ecological balance of rice fields due to the development of rice planthopper resistance and the extensive use of chemical pesticides. , seriously weakening the natural control ability in the field, which will easily lead to the rampant and difficult control of rice planthoppers. Therefore, it is of great significance to develop s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 夏玉先彭国雄曾德玉
Owner 成都中农劲威生物科技有限公司
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