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A strain of Metarhizium anisopliae pathogenic to the Laotian beetle and its application

A technology of Acanthus acanthus and Metarhizium anisopliae, applied in application, fungi, insecticides, etc., can solve the problems of differences in pathogenicity of target pests, and achieve reduced larval populations, strong pathogenicity, and sporulation powerful effect

Active Publication Date: 2018-08-17
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although Metarhizium anisopliae has a wide range of hosts, studies have found that the pathogenicity of strains of different geographical and host sources to target pests is very different. 50 ) can differ by several times, or even tens of times, therefore, screening out strains with strong pathogenicity for different pests is the basis for using Metarhizium anisopliae to control pests

Method used

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  • A strain of Metarhizium anisopliae pathogenic to the Laotian beetle and its application
  • A strain of Metarhizium anisopliae pathogenic to the Laotian beetle and its application
  • A strain of Metarhizium anisopliae pathogenic to the Laotian beetle and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Metarhizium anisopliae ( Metarhizium anisopliae ) Morphology of FJMa200902

[0031] Inoculate the isolated and purified Metarhizium anisopliae strains on a flat PPDA medium with a diameter of 90mm and inoculate them by the spot method, and then culture them at 25±1°C. After 4-5 days, pick mycelia to make slices and observe the strains under a microscope. Morphology of hyphae and conidiophores; 10-12 days later, pick mature conidia and make slices to observe the shape and size of conidia. Observe and record the morphological characteristics of the colony for about 15 days. The colony texture of this strain is cotton-wool, and the colony is white at the beginning, and gradually expands, and dark green conidia are produced on it, which are distributed in rings and rays. Mycelia branched, gridded, colorless and smooth, 2.3μm~3.2μm wide, conidiophores solitary or branched, with 2~5 columnar sporulation cells at the top of the branches, on which basal sporozoites are produ...

Embodiment 2

[0034] Metarhizium anisopliae ( Metarhizium anisopliae ) Biological properties of FJMa200902

[0035] Metarhizium anisopliae ( Metarhizium anisopliae ) FJMa200902 was inoculated in the center of the PPDA plate medium, cultured in an incubator at 25±1°C, and the colony diameter was measured once a day by the cross method, and 3 plates (repeated) were set up for each treatment, and observed for 15 days in total. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5 cm to punch out a small plate from the center of the colony to the edge 1 / 2, take out the plate and place it in a 30 mL container In a conical flask with 0.3‰ Tween-80 sterile water, shake to fully disperse the spores and dilute them appropriately. Measure the spore concentration with a hemocytometer and convert it into the sporulation per unit area. Three bacterial discs were measured on each plate, and the average value was t...

Embodiment 3

[0040] Metarhizium anisopliae ( Metarhizium anisopliae ) Determination of the pathogenicity of FJMa200902 to the larvae of the Laotian beetle

[0041] A certain amount of 0.03% Tween-80 solution was prepared, then sterilized at high temperature, and cooled for later use. Scrape a certain amount of Metarhizium anisopliae from the PPDA plate ( Metarhizium anisopliae ) FJMa200902 spores were placed in the above solution and prepared into 10 7Spores / mL of spore suspension; immerse healthy Laotian beetle larvae in the spore suspension for 10 seconds, then put them back into the insect culture box and feed them with sterilized sawdust, and inoculate 30 Laotian beetle larvae , repeated 3 times; soaking 0.3‰ Tween-80 sterile aqueous solution as a blank control, and inoculating Metarhizium anisopliae MaZPTR-01 strain (a strain preserved by Fujian Academy of Forestry, a known strain) as a control, the inoculation method was the same as that of Concentrations are the same as above. ...

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Abstract

The invention discloses a metarhizium strain pathogenic to Laos false blister beetles and application of the metarhizium strain. The metarhizium strain is named as metarhizium anisopliae [FJMa200902, which is stored in China general microbiological culture collection center (CGMCC)], and the collection number is CGMCC No.10642. The strain grows fast and has strong spore formation capability, good production traits and high pathogenicity to larvae of the Laos false blister beetles; after the strain is inoculated by a spore suspension liquid of 107 spores / mL, LT50 (lethal time of 50%) of the strain to the larvae of the Laos false blister beetles is 3.6 days, and infection rate reaches 100%. A fungus preparation made from the strain has good prevention and treatment effects on the Laos false blister beetles, population density of the larvae of the Laos false blister beetles can be reduced effectively, and the metarhizium strain is a biocontrol strain which has application value for prevention and treatment of the Laos false blister beetles.

Description

technical field [0001] The invention belongs to the field of biological control, and relates to a metarhizium anisopliae strain and its application, in particular to a metarhizium anisopliae strain with high pathogenicity to the Laotian beetle beetle and its application. Background technique [0002] Laotian beetle Neacanista laosensis (Breuning, 1965) comb. nov., once named short longhorn beetle Paracanthocinus laosensis Breuning, Huang et al. in 2015 classified it into the genus Pseudomonas Neacanista , named as the Laotian beetle. This insect is an important borer pest on the Fagaceae plants such as Castanopsis chinensis and sweet mulberry. In a serious tree, hundreds of larvae gather to eat the sapwood of the trunk and eat back and forth in the trunk or phloem, causing Substantial water and nutrient transport disruptions, and it can be associated with the bully beetle Aphredisinm faldermannii rufiventris Co-occurrence, once the host plant is harmed, the mild o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04
Inventor 何学友蔡守平苏文晶钟景辉陈曜
Owner FUJIAN ACAD OF FORESTRY
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