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Pesticidal Metarhiziumanisopliaevar. anisopliae strain and application thereof

A technology of Metarhizium anisopliae and Metarhizium anisopliae, applied in the direction of fungi, microorganisms, and methods based on microorganisms, can solve the problems of lack of high-virulence insecticidal fungal strains, etc., to achieve population control, good insecticidal effect, The effect of strong sporulation ability

Active Publication Date: 2012-08-01
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insecticidal fungi have strong vertical and horizontal diffusion capabilities, are easy to form epidemics, and are conducive to the continuous control of pests. They are especially beneficial to the control of migratory pests, the rice leaf roller. No related reports have been seen yet, one of the main reasons is the lack of highly virulent insecticidal fungal strains

Method used

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  • Pesticidal Metarhiziumanisopliaevar. anisopliae strain and application thereof
  • Pesticidal Metarhiziumanisopliaevar. anisopliae strain and application thereof
  • Pesticidal Metarhiziumanisopliaevar. anisopliae strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation of bacterial strains

[0016] Disinfect the surface of the collected naturally-infected rice leaf roller worms with 70% alcohol, put them in a 28°C incubator for 3-5 days, and make fresh spores grow on the surface of the insects; under sterile conditions, Use an inoculation loop to pick a small amount of fresh spores and streak them on a 1 / 4 SDA medium plate containing 100 μg / ml ampicillin; turn the streaked culture dish over and place it in a 28°C incubator for cultivation; after 3-4 days, select long For the typical colony without miscellaneous bacteria, pick a small piece of culture medium with hyphae at the edge of the colony with a transfer needle, transfer it to the center of the culture medium on the slant of the test tube, and cultivate it at a constant temperature of 28°C for 10-15 days.

Embodiment 2

[0017] Identification of embodiment 2 strains

[0018] (1) Morphological identification

[0019] The isolated CQMa421 strain was inoculated on a 1 / 4 SDA plate and cultured at 28°C to observe the morphological characteristics of the colony, the sporulation structure and the shape and size of the spores. The results showed that the colony was white cotton flocculent at the initial stage of culture on 1 / 4SDA medium, and olive green when sporulation. Mycelium is septate and branched, transparent, 1.5-2.0μm in diameter. Conidia spores are solitary or clustered or closely arranged, broom-like branched, about 2.0 μm in diameter, and bottle-shaped peduncles, 8-16 × 1.5-2 μm, are produced at the ends. Chains of conidia form in basal succession from the end of the bottle stem (cf. figure 1 left picture in ). Conidia unicellular, oblong, 4.8-7 × 2-3 μm in size (see figure 1 right picture in ). From the above characteristics, the strain CQMa421 should be a var. microsporum of Me...

Embodiment 3

[0023] Example 3 Determination of Indoor Insecticidal Activity of CQMa421 on Rice Leaf Roller

[0024] Collect the mature spores of CQMa421 cultured on 1 / 4SDA plates, disperse them with 0.05% (W / V) Tween-80, and make a final concentration of 1×10 8 spores / ml suspension. The larvae of Leaf Roller Borer were dripped, and each test worm was dripped with 2 μl of the drug with a micro-spotter, and 30 larvae were inoculated. After inoculation, they were reared at a room temperature of 28°C and a humidity of 50-70%, with 5 repetitions. Clear water was used as blank control. Record the number of dead insects, calculate the survival rate, and obtain the fungal LT by statistical analysis of DPS2000 software 50 (when lethal) and LT 90 (lethal time).

[0025] from image 3 It can be seen that the Metarhizium anisopliae CQMa421 strain has obvious insecticidal activity against the rice leaf roller. At the peak of death 5-7 days after inoculation, the larvae of the rice leaf roller b...

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Abstract

The invention relates to a Metarhiziumanisopliaevar. anisopliae strain CQMa421 of which the CGMCC (China General Microbiological Culture Collection Center) NO is 4609 and an application thereof in the control of rice leaf roller. The production traits of the strain are good and can grow fast and have high spore-producing capability; and the sporulation rate is up to 6.8%, and the strain has high toxicity. The pesticidal fungal agent produced by using the strain can be used to effectively control the number of the rice leaf rollers.

Description

technical field [0001] The invention relates to a new microbial strain, in particular to an insecticidal Metarhizium anisopliae strain and application thereof, belonging to the field of biological pesticides. technical background [0002] The rice leaf roller is one of the main pests of rice. The domestic rice leaf roller is widely distributed in various rice areas of the country, seriously threatening my country's grain production. At present, broad-spectrum chemical pesticides are mainly used to control rice leaf roller, which not only directly affects human health and the safety of aquaculture industry, but also because rice leaf roller produces drug resistance and chemical pesticides kill a large number of rice leaf roller's natural enemies, Destroy the ecological balance of rice fields, seriously weaken the natural control ability of the field, and easily lead to the rampant and difficult control of the rice leaf roller. [0003] Entomopathogenic fungi are the most imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 夏玉先彭国雄
Owner CHONGQING UNIV
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