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Metarhizium anisopliae and application thereof

A technology of Metarhizium anisopliae and fungal agents, applied in the fields of application, fungi, methods of using spores, etc., to achieve good effects, strong sporulation ability, and strong pathogenicity

Active Publication Date: 2014-05-28
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain was preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures (CGMCC for short) on April 06, 2012.

Method used

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  • Metarhizium anisopliae and application thereof
  • Metarhizium anisopliae and application thereof
  • Metarhizium anisopliae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Isolation and Identification of Metarhizium anisopliae JF-6213

[0035] 1. Isolation of Metarhizium anisopliae JF-6213

[0036] Weigh 10g of different soil samples from the surface soil of the Jianfengling forest in Ledong, Hainan, China, and put them into 50mL sterile triangular flasks filled with 20mL of sterile water, stir and mix well, and let stand for a while. After the soil settles, absorb Transfer 5mL supernatant to a 10mL sterile centrifuge tube for later use.

[0037] Take the prepared sterilized oat agar medium, add 34mg / mL chloramphenicol solution at about 50°C to make the concentration of chloramphenicol in the medium reach about 500mg / L, shake well and pour the oat agar medium Pour about 200mL of culture medium into a 25cm×15cm×3cm sterile stainless steel square dish. After the medium was cooled and solidified, 4 mL of supernatants from different soil samples were taken and inoculated evenly on different square plates with a coating stick. Aft...

Embodiment 2

[0047] Example 2. Determination of Indoor Insecticidal Activity of Metarhizium anisopliae JF-6213 CGMCC No.5952 on Sugarcane Borers

[0048] The isolated and purified Metarhizium anisopliae (Metarhizium anisopliae) JF-6213 CGMCC No.5952 strain was inoculated on a PDA plate and cultured at 28°C for 8-9 days; the mature spores cultivated on the PDA plate were collected and washed with 0.05% (0.05 g / 100ml) dispersed in Tween-80 aqueous solution and prepared to a final concentration of 1×10 8 spores / ml (1×10 8 cfu / ml), the suspension is the inoculant of Metarhizium anisopliae.

[0049] Two treatments were set up in the experiment, which were the inoculum group of Metarhizium anisopliae (referred to as the inoculum group) and the control group. Each treatment was replicated 3 times, and each replicate had 2 sugarcane seedlings.

[0050] 30 sugarcane borers (Chilo venosatus) (23 in the 3rd instar and 7 in the 4th instar) with similar body size and healthy 3rd to 4th instar were p...

Embodiment 3

[0059] Example 3. Outdoor control effect of Metarhizium anisopliae JF-6213 CGMCC No.5952 on sugarcane borer

[0060] The experiment was set in the plots that had been harmed by sugarcane stem borers in the previous year. Two treatments were set up in the experiment, namely the Metarhizium anisopliae inoculum group (Treatment 1) and the control group (Treatment 2). 100 rows (5 acres) per treatment.

[0061] The Metarhizium anisopliae fungus group was planted with the Metarhizium anisopliae fungus fertilizer, and the Metarhizium anisopliae fungus fertilizer was prepared according to the following method: the isolated and purified Metarhizium anisopliae JF-6213CGMCC No.5952 The strain was inoculated on a PDA plate and cultured at 28°C for 8-9 days; the mature spores cultured on the PDA plate were collected and dispersed with 0.05% (0.05g / 100ml) Tween-80 aqueous solution to prepare a final concentration of 1×10 8 spores / ml (1×10 8 cfu / ml) suspension. After getting 10L of suspe...

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Abstract

The invention discloses Metarhizium anisopliae and application of Metarhizium anisopliae. The Metarhizium anisopliae provided by the invention is Metarhizium anisopliae JF-6213 with CGMCC No. 5952 registered in China General Microbiological Culture Collection Center. The strain (Metarhizium anisopliae) has good production traits and strong spore production capacity, strong insecticidal activity to sugarcane moth borer, good secondary infection to sugarcane moth borer, has 7.44 days LT50 (lethal time of 50%) to 3-4 years sugarcane moth borer; the strain is used for preparing insect killing fungus preparation which can effectively control population quantity of sugarcane moth borer; moreover, Metarhizium anisopliae JF-6213 has strong pathogenicity to brontispa longissima, asiatic migratory locust, leechee stinkbug and Dysmicoccus neobrevipes Beardsley, strong secondary infection capability and high stability and is quite applicable to tropical and subtropical areas.

Description

technical field [0001] The invention relates to a strain of Metarhizium anisopliae and its application. Background technique [0002] Sugarcane stem borer (sugarcane stem borer), commonly known as core borer, belongs to the order Lepidoptera of the insect class Lepidoptera. Among them, there are 50 species of Mothidae insects, 12 species of Noctuidae, 1 species of Toritridae, 1 species of Pterodidae and 1 species of Pterodidae. The field density of this insect is high, and the whole growth period of sugarcane from sowing to harvesting is harmed by it. After being damaged at the seedling stage, the seedlings cannot germinate and unearth; The number and effective stems are reduced, and the dead heart rate is generally 10% to 20%, and the serious field can reach more than 60%. In the middle and late stages of sugarcane growth, larvae will damage the sugarcane stems, destroy the tissue of the sugarcane stems, and affect the growth of sugarcane. In strong winds, sugarcane stems...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P7/04C12R1/645
Inventor 黄俊生郭立佳梁昌聪杨腊英刘磊
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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