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A kind of Beauveria bassiana dsxj‑07 and its application

A technology of DSXJ-07 and Beauveria bassiana, which is applied in the field of bioengineering, can solve problems such as unsatisfactory insecticidal effects, and achieve the effects of good production traits, rapid growth, and population control

Active Publication Date: 2018-03-20
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, entomopathogenic fungi have strong vertical and horizontal diffusion capabilities, which contribute to the continuous control of pests, and are particularly effective in controlling the migratory pest rice water weevil. Although the use of insecticidal fungi to control rice water weevil has been seen Related reports, but insecticidal effects are not satisfactory, one of the main reasons is the lack of entomopathogenic fungal strains with high toxicity to rice water weevil

Method used

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  • A kind of Beauveria bassiana dsxj‑07 and its application
  • A kind of Beauveria bassiana dsxj‑07 and its application
  • A kind of Beauveria bassiana dsxj‑07 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Isolation and identification of Beauveria bassiana bacterial strain DSXJ-07

[0018] The collected naturally infected rice water weevil beetles were first immersed in 75% alcohol for 3-5 seconds, then surface-sterilized with 0.5% NaClO for 3 minutes, rinsed with sterilized water for 3 times, and wiped with a sterilized knife under aseptic conditions. Infected worms were cut into small pieces of 5 mm square, placed on 9 cm PDA plates for culture, and placed in an incubator at 28 ° C for 3 to 5 days to make fresh spores grow on the surface of the insects; then use an inoculation loop to pick fresh spores containing Streak on a PDA plate with 50 μg / ml cephalosporin and 30 μg / ml kanamycin; place the streaked culture dish upside down in an incubator at 28°C; pick out typical colonies without bacteria after 3 to 4 days , use an inoculation loop to pick a small piece of medium with mycelium at the edge of the colony, transfer it to the center of the plate medium, and...

Embodiment 2

[0025] Example 2 Beauveria bassiana DSXJ-07 to indoor insecticidal activity determination of rice water weevil

[0026] Collect the mature spores of Beauveria bassiana DSXJ-07 cultured on the PDA plate, disperse them with 0.05% (W / V) Tween-80, and prepare a final concentration of 1×10 7spores / ml suspension. The rice water weevil adults collected from the field were raised in 50ml centrifuge tubes with air holes containing rice seedlings, 50 rice water weevils per tube, and the rice water weevils were immersed in the spore suspension for 1-2 seconds for inoculation. After inoculation, in an incubator at 28° C., RH=90%, the experiment was set up for 3 repetitions, and clear water was used as a blank control. Record the number of dead insects every day, calculate the survival rate of rice water weevil, and get LT through statistical analysis of DPS software 90 value.

[0027] The experimental results showed that Beauveria bassiana DSXJ-07 had obvious insecticidal activity agai...

Embodiment 3

[0030] Example 3 Field efficacy test of Beauveria bassiana wettable powder for controlling rice water weevil

[0031] The DSXJ-07 strain Beauveria bassiana high sporopollen was formulated into 9 billion / g Beauveria bassiana wettable powder, prepared with a sprayer at a concentration of 9 billion / g Beauveria bassiana wettable powder, and sprayed with water in the field. Control agent (biological pesticide - matrine, chemical pesticide - water amine. Marathon)

[0032] This experiment was arranged in the paddy field of Dayushu Village, Nanweizi Town, Gongzhuling City, Jilin Province. The amount of rice water weevil A. chinensis was investigated before the drug was applied. The drug was applied 7 days after rice transplanting, and the drug was applied again after an interval of 7 days. 3 days after spraying, the amount of thymesis was investigated and the control effect was calculated. The test results are shown in Table 2:

[0033] Table 2 Efficacy test of Beauveria bassiana w...

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Abstract

The invention discloses a Beauveria bassiana DSXJ-07, which has been preserved, and the storage number is CGMCC No.7192, and the sequence of the internal transcriptional spacer is SEQ ID No.7192. The present invention also provides the application of the bacterial strain for preventing and controlling pests on gramineous crops, and as the application of preparing a medicine for preventing and controlling pests on gramineous crops, wherein the gramineous crops are rice, corn, sugarcane, wheat, barley or forage grass. The pests mentioned are rice water weevil and Asian corn borer. The insecticidal fungal preparation produced by the strain can effectively control the populations of the rice water weevil and the Asian corn borer.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Beauveria bassiana strain DSXJ-07 and the application of the strain for preventing and controlling crop pests. Background technique [0002] Rice water weevil (Lissorhoptrus oryzophilus Kuschel) belongs to Coleoptera, Weevilidae, Marsh Weevil subfamily, and Oryzophilus genus. It mainly harms rice and uses corn, sugarcane, wheat, barley, pasture grass, grass weeds, etc. as hosts. It has the characteristics of long damage time, fast propagation speed, strong drug resistance and stress resistance. Adults eat rice leaves, and larvae eat rice roots, which generally cause a 15% to 20% reduction in rice production, and more than 50% in severe cases, or even no harvest. It is an important quarantine pest on rice and is listed as the world's top 100 species by the International Union for Conservation of Nature. One of the most threatening invasive alien species. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 田志来高月波李启云
Owner JILIN ACAD OF AGRI SCI
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