A strain of Metarhizium anisopliae for controlling Camellia oleifera larvae and its application

A technology for Metarhizium anisopliae and Camellia oleifera, which is applied in the directions of application, pesticides, fungi and the like, and achieves the effects of strong pathogenicity, good application prospects and good production characteristics

Active Publication Date: 2017-08-25
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the search, there is no report on the use of entomogenic fungi to control Camellia oleifera larvae. For this reason, Metarhizium anisopliae with strong pathogenicity to its larvae was screened out ( Metarhizium anisopliae ) strains, it is of great practical significance to provide excellent strains and bacterial agents for production

Method used

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  • A strain of Metarhizium anisopliae for controlling Camellia oleifera larvae and its application
  • A strain of Metarhizium anisopliae for controlling Camellia oleifera larvae and its application
  • A strain of Metarhizium anisopliae for controlling Camellia oleifera larvae and its application

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Experimental program
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Embodiment 1

[0022] Metarhizium anisopliae ( Metarhizium anisopliae ) Identification of FJMa201101

[0023] Morphological identification

[0024] Cultivate the isolated and purified Metarhizium anisopliae strain on a flat PPDA medium with a diameter of 90mm at 25±1°C. After 4-5 days, pick a white hyphae preparation and observe the hyphae and meristems of the strain under a microscope Spore peduncle morphology; after 10-12 days, pick mature conidia and make slices to observe the morphology and size of the conidia. Observe and record the morphological characteristics of the colony for about 15 days. The colony texture of this strain is cotton-wool, and the colony is white at the beginning, and gradually expands, and dark green conidia are produced on it and distributed in a ring. Mycelia branched, latticed, colorless and smooth, 2.3μm~3.2μm wide, conidiophores solitary or branched, with 2~5 columnar sporulation cells at the top of the branches, on which basal sporozoites are produced Co...

Embodiment 2

[0034] Metarhizium anisopliae ( Metarhizium anisopliae ) Biological properties of FJMa201101

[0035] Metarhizium anisopliae ( Metarhizium anisopliae ) FJMa201101 was inoculated in the center of the PPDA plate medium, cultured in an incubator at 25±1°C, and the colony diameter was measured once a day by the cross method, with 3 plates (repeated) for each treatment, and observed for 15 days in total. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5 cm to punch a small plate from the center of the colony to the edge 1 / 2, take out the plate and place it in a 0.3 ‰ Tween-80 sterile water in the Erlenmeyer flask, shake to fully disperse the spores and properly dilute, measure the concentration of spores with a hemocytometer and convert it into the amount of sporulation per unit area. The determination was repeated three times on each plate, and the average value was taken as the number ...

Embodiment 3

[0040] Metarhizium anisopliae ( Metarhizium anisopliae ) Determination of pathogenicity of FJMa201101 to Camellia oleifera larvae

[0041] Scrape a certain amount of Metarhizium anisopliae from the PPDA plate ( Metarhizium anisopliae ) FJMa201101 spores were placed in sterile 0.3‰ Tween-80 solution, and prepared into 10 7 Spores / mL of spore suspension (the suspension is Metarhizium anisopliae FJMa201101 inoculum); soak healthy Camellia oleifera larvae in the spore suspension for 10 seconds, and then put them back into the sterilized soil in the insect culture box In this method, 30 camellia oleifera larvae were inoculated and repeated 3 times; treated with 0.3‰ Tween-80 sterile aqueous solution as a control. After treatment, place the insect rearing box in a dark 25±1°C constant temperature incubator, observe and record the death of camellia oleifera larvae every day, observe the growth of mycelia and sporulation of the dead insects in moisturizing culture (the growth of b...

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Abstract

The invention discloses a strain of Metarhizium anisopliae for controlling camellia oleifera larvae and its application. The Metarhizium anisopliae strain provided by the present invention is named as Metarhizium anisopliae (Metarhizium anisopliae) FJMa201101, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on October 27, 2014, and the preservation number is CGMCC No.9780 . The strain has fast growth rate, strong sporulation ability, and high pathogenicity to the larvae of Camellia oleifera. The LT50 (medium lethal time) to larvae of Camellia oleifera after inoculation with 107 spores / mL larvae was 1.65 days. The fungal preparation produced by the strain has a good control effect on Camellia oleifera and can effectively control the population density of Camellia oleifera.

Description

technical field [0001] The invention belongs to the field of biological control, and relates to a strain of Metarhizium anisopliae and its application, in particular to a strain of Metarhizium anisopliae with high pathogenicity to larvae of Camellia oleifera and its application. Background technique [0002] Camellia oleifera (Oil-tea Camellia) is a unique woody edible oil tree species in my country, mainly distributed in the Yangtze River Basin and 14 provinces (cities, districts) south of it. Camellia elephant Curculio chinensis Chevrolat, also known as Tea Seed Weevil and Camellia oleifera, belongs to the Weevil family Weevil. It is the most serious pest that harms Camellia oleifera fruit, and it is also a national forestry dangerous pest (State Forestry Administration, 2013). The larvae eat nuts in the fruit, which seriously reduces the yield of tea seeds and affects the quality of tea oil. According to the survey, the fruit drop rate after Camellia oleifera damage can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 何学友蔡守平杨希曾丽琼黄金水
Owner FUJIAN ACAD OF FORESTRY
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