Novel Aspergillus niger strain and application thereof

A strain of Aspergillus niger, a new type of technology, applied in the field of microorganisms, to achieve the effects of strong spore production, good industrial production performance, and fast growth

Inactive Publication Date: 2017-02-15
烟台启元微生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the use of one-step method for the preparation of starch sugar at home and abroad

Method used

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  • Novel Aspergillus niger strain and application thereof
  • Novel Aspergillus niger strain and application thereof
  • Novel Aspergillus niger strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and isolation of novel Aspergillus niger strain QY-002.

[0029] The novel Aspergillus niger strain QY-002 described in the present invention comes from the intermountain humus soil at an altitude of 1500-2000 meters. After the initial screening of the medium and the re-screening of the enzyme production medium, a strain with excellent growth performance is obtained. The strain can utilize starch, produce amylase and glucoamylase at the same time, and the two enzymes have high enzyme activity, and are stored on the wort slant medium.

[0030] Preparation method of wort slant medium: weigh 300g of barley malt and add 700ml of water, after twice saccharification to obtain wort with a Baume degree of 7-12, then add 2% agar, and sterilize for later use.

Embodiment 2

[0031] Example 2 Identification of novel Aspergillus niger QY-002 strains.

[0032] 1) Microbiological characteristics: It spreads rapidly on the special wort medium, and colonies appear within 24 hours. They are initially white, then turn yellow-green to black, velvet-like, with a slightly raised center, and the opposite side of the colony is slightly yellowish-white. After 72 hours, the spores were abundant, the overall shape of the conidia was like a broom, the conidiophores were of different lengths, and the conidiospores were spherical and black.

[0033] 2) Molecular biological characteristics: the strain was identified as Aspergillus niger by 18S rRNA sequence, and the sequence table is shown in SEQ ID NO.1.

Embodiment 3

[0034] Example 3 Rejuvenation and liquid fermentation of novel Aspergillus niger QY-002 to prepare amylase and glucoamylase enzyme solutions.

[0035] 1) Rejuvenation: the strain was inoculated on wort slant medium and cultured at 28-34°C for 7 days.

[0036] Preparation method of wort medium: weigh 400g of barley malt and add 600ml of water, after twice saccharification to obtain wort with a Baume degree of 7-12, then add 2% agar, and sterilize for later use.

[0037] 2) Liquid culture: the wort slant is inoculated into the shake flask liquid medium.

[0038]Culture conditions: temperature 28-32°C, rotation speed 160-200rpm, culture time 68-78 hours. Filter through four layers of gauze, and take the filtrate for later use. The amylase activity in the filtrate can reach 1.8 u / ml, and the glucoamylase activity can reach 40 u / ml.

[0039] Medium formula: per 1L medium (pH=5.5~5.8): 7.28g sodium nitrate, 0.8g potassium dihydrogen phosphate, 0.4g magnesium sulfate heptahydrate,...

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Abstract

The invention provides a novel Aspergillus niger strain QY-002 capable of simultaneously secreting amylase and glucoamylase. The novel Aspergillus niger strain QY-002 has an accession number of CGMCC No. 10650 and is preserved on April 24, 2015. In the conventional fermentation industry, amylase and glucoamylase are separately produced by using different processes. In the conventional sugar industry, liquefaction and saccharification of starch are carried out in two steps under different temperature and pH value conditions and aided by amylase and glucoamylase respectively. Enzyme liquid obtained after fermentation of the novel Aspergillus niger strain has the activity of both amylase and glucoamylase and produces synergism at a same reaction temperature and a same pH value condition, so high-efficiency catalysis is realized in complete hydrolysis of a substrate, i.e., starch, into glucose. Thus, a process of preparing sugar from starch is greatly simplified; reaction time is shortened; product quality is improved; energy consumption is reduced; production cost is lowered; and the novel Aspergillus niger strain has potential and wide application value and is of epoch-making significance to development of the industry of preparation of sugar from starch in China.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. Specifically, it relates to a novel Aspergillus niger strain QY-002 that simultaneously produces amylase and glucoamylase. The obtained fermentation broth has dual enzyme activities of amylase and glucoamylase, and has important uses in the field of starch sugar production. Background technique [0002] my country is a large agricultural country, with a wide area of ​​grain planting, large output, and high starch content, but the price is low. In order to increase the economic added value of grains and maximize economic benefits, most of them require intensive processing. Among them, starch sugar is one of the main ways of grain deep processing. [0003] Starch sugar is the best substitute for sucrose, mainly including maltose, glucose, fructose, etc. It is widely used in food, biomedicine, chemical and fermentation industries and other fields. At present, my country's starch sugar prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/30C12N9/34C12P19/22C12P19/14C12P19/02C12R1/685
Inventor 况代武
Owner 烟台启元微生物技术研究院有限公司
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