55 results about "Mucor circinelloides" patented technology

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

The invention provides a preparation method of a mixed microbial agent, the mixed microbial agent, and an application of same, and belongs to the technical field of environmental microorganisms. The preparation method includes: activating Mucor circinelloides, Bacillus tequilensis and Bacillus velezensis, mixing the spores of the Mucor circinelloides with the other two bacteria according to ratioof 1:3:3, and inoculating the mixed bacteria on a CPDA culture medium, culturing the mixed bacteria at 28 DEG C for 48 h in a constant temperature shaking table at 150 rpm to obtain a mixed microbialliquid. In the invention, by means of the character that the Mucor circinelloides is high in growth speed and can carry and diffuse bacteria, the Mucor circinelloides, applied to the aerobic composting of organic wastes, can enhance the diffusion of organic waste degrading bacteria in a heap, so that composting period is shortened and composting efficiency is increased effectively. Compared with simple microbial agent, the mixed microbial agent has great adaptability and stable performance, and has an extensive application prospect of quick composting reutilization of the organic wastes.

The invention discloses fecal deodorizing microorganism combining bacteria and a method for producing an organic fertilizer using same. The combining bacteria comprise the following components: Mucor circinelloides (Mucor Circinelloides), Bacillus pumilus (Bacillus Pumilus), and Stenotrophomonas maltophilia (Stenotrophomonas maltophilia), the number ratio of which is 1:0.9-1.2:0.9-1.2. The manure and straws are used as raw materials, and the microorganism combining bacteria are fermented to produce the organic fertilizer. In the process of producing the organic fertilizer by the method, the odor generation can be obviously reduced in the compositing process, and the method has simple process, low cost and easy promotion.

The invention discloses a Mucor circinelloides lusitanicus AY234877 strain which has the stereoselective hydroxylase activity, which is collected in the China general microbiological culture collection center on November 20, 2007, the collection number is CGMCC.NO.2256, and the invention is characterized in that the positions of 6 Beta, 7 Alpha, 11 Alpha and 14 Alpha of C21 and C19 steroid substrates can be carried out a hydroxylation. The invention further discloses a method for preparing the 6 Beta, 7 Alpha, 11 Alpha and 14 Alpha hydroxylated derivatives of the C21 and C19 steroids and aza steroids. The method has simple operation, low cost and better yield and stereoselectivity; furthermore, the invention does not cause environmental pollution. The strain can carry out the hydroxylation reaction of the C21 and C19 steroids and aza steroid compounds which have the structural characteristics of 4-en-3-one or 5-en-3-ol, and the obtained hydroxylated derivatives are the important intermediates for the synthesis of a plurality of steroid compounds. Compared with the steroid hydroxylation chemical synthesis, the method can simplify drug synthetic steps, reduce pollution, reduce cost and have important application value for promoting the development process of the steroid drug intermediates of China.

The invention provides a superior nickel-rich fungus resistant to aluminum profile electronickelling bath solution, in particular to screening and application of mucor circinelloides FNZJ3-2-2. The mucor circinelloides FNZJ3-2-2 is preserved in China General Microbiological Culture Collection Center on September 19, 2014, and the preservation number is CGMCC No.9684. The mucor circinelloides FNZJ3-2-2 is capable of withstanding pH ranging from 4.2 to 4.5 and excellent in nickel enrichment performance and growth activity and has promising application prospect in biological purification of the aluminum profile electronickelling bath solution.

The invention discloses mucor circinelloides, a method for preparing viscosity-reduction enzyme and application of viscosity-reduction enzyme, and belongs to the field of microbe and microbial fermentation. Aiming at the disadvantages of reducing sweet potato fermentation liquor viscosity in the prior art, the invention provides a strain capable of producing viscosity-reduction enzyme. Mucor circinelloides CBS131818 is preserved in Royal Netherlands Academy of Arts and Sciences Centraalbureauvoor Schimmelcultures, and the preservation date is Jan. 27, 2012. Mucor circinelloides CBS131818 is acquired from acid-processed paddy rice straw treating fluid and is applicable to preparation of viscosity-reduction enzyme. The produced viscosity-reduction enzyme is high in activity, is further applicable to viscosity reducing technology of sweet potato fuel ethanol fermentation liquors, is capable of effectively reducing the viscosity of the sweet potato stem-tuber fermentation liquor with small use amount and short action time, and helps to simplify the sweet potato fuel ethanol fermentation technology and reduce production cost.

The invention discloses a bacterial strain for producing polyunsaturated fatty acids (PUFAs) and a screening method thereof. The bacterial strain is named as Mucor circinelloides in taxonomy, and the preservation number of the bacterial strain is CGMCC No.8361. The screening method comprises the following steps: (1) prescreening at a low temperature; (2) dyeing the bacterial strain with Sudan black and rescreening; (3) fermenting and culturing the bacterial strain in liquid; and (4) using gas chromatography for detecting the content of fatty acids in bacterial strain cells. The bacterial strain obtained by the screening method disclosed by the invention can produce the polyunsaturated fatty acids in high yield. Through the adoption of the screening method disclosed by the invention, the bacterial strain can be obtained quickly and repeatedly, so that the screening method has the advantages of low cost and capability of being applied to industrialized production, and the like.

The invention relates to a method for preparing flax fiber by a combined process of a mucor circinelloides DK1 strain and hydrogen peroxide. The method comprises the following steps: (1) culturing the strain; (2) preparing a crude enzyme solution; and (3) mixing a flax raw material after sterilization and a mixed solution of the crude enzyme solution and hydrogen peroxide according to the mass-to-volume ratio of 1: (10-30), regulating a pH value, then putting into a shaking bed for treatment, finally adding sodium hydroxide, increasing the temperature, degumming, removing a degumming solution, washing, stopping washing when the pH value is natural, and completing degumming. The strain adopted by the invention is short in growth period, the strain is less liable to pollution, the treatment cost is low, and the degumming condition is mild; and the degumming method is simple in process flow, the time is shortened, the energy consumption is reduced, the pollution emission is reduced, and the method is conductive to environmental protection.

The invention provides a method for synthesizing 7,21-dyhydroxyl-20-methyl pregnane-4-alkene-3-ketone by microorganisms. Through a two-step fermentation method, one or more kinds of fermentation liquor including mucor circinelloides lusitanicus, acremonium strictum, mucor.racemosus and aspergillus fumigatus can be used for efficiently converting 4-HBC into 7 alpha-OH-4-HBC and 7beta-OH-4-HBC. Under a condition that the inventory of substrate 4-HBC is 10-20 g / L, a conversion rate is high, two products, including 7alpha and 7beta-hydroxy-4-HBC are separated from the product, and the total yieldof the product is 46.5% to 85.32%. The method disclosed by the invention is convenient in operation, low in cost and high in yield, and has good economic value, and a new method is provided for the development and the synthesis of steroid drugs.

The invention relates to the technical field of biology, in particular to a primer set and an application thereof, and discloses a primer combination for 4 fungi LAMP of mucorales and an application of the primer combination. The primer combination provided by the invention consists of 24 primers as shown in SEQID No.1-24. The primer combination provided by the invention can be applied to detect whether rhizopus oryzae, lichtheimia corymbifera, mucor circinelloides and / or rhizomucor pusillus exist or not, and can be applied to whether samples to be tested contain the rhizopus oryzae, the lichtheimia corymbifera, the mucor circinelloides and / or the rhizomucor pusillus exist or not. The primer combination provided by the invention is used for 4 fungi of the mucorales, has high specificity and sensitivity, and can realize simple, quick and accurate detection. The primer set has important promotion value.

The invention relates to a microbial agent. The microbial agent is formed by three fungi including mortierella, trichoderma asperellum and mucor circinelloides. The fungi are activated, propagated anddiluted into 1-9*10<7>CFU / ml fungal liquid respectively, and the three types of fungal liquid are subjected to isovolumetric mixing to obtain the microbial agent. The application of the microbial agent to passivation of heavy metals in soil refers to a method of plant infection with the microbial agent to form infected okra, alfalfa and soybean plants, and removal rates of copper, zinc, lead, cadmium and manganese is 75.69%, 57.60%, 89.61%, 82.83% and 68.77% respectively. Preparation and application methods of the microbial agent are simple and economical, soil physiochemical properties can be effectively improved, fertilizer and water retention capacity of soil is increased, secondary pollution is avoided, and a short application period is realized.

The invention relates to a method for planting and greening of tailing by a microbial agent and a modifier. The tailing is covered with soil dressing with the thickness of 2-4cm, the modifier made ofcharcoal, organic fertilizer, husk and haycite is applied, wherein the dosage is 2.0-2.5kg / m<2>; turning cover is conducted, wherein the depth of turning over is 15-20cm; after turning over, the microbial agent containing mortierella, trichoderma asperellum and mucor circinelloides is sprayed, wherein the dosage is 15-20g / m<2>; leveling and watering are conducted, the water holding rate of the tailing is 60%+ / -5%; and okra, alfalfa and kochia scoparia are planted, harvesting is conducted after 2-3 months. The method solves the problem that mass waste tailing produced by mining is hard to use.The adopted microbial agent is screened from the tailing, adaptation is achieved. The modifier is a green and non-polluting material, and the cost is low. Soil utilization of the tailing is achieved,and secondary pollution in the process of tailing utilization can be eliminated.

The present invention relates to a construction method and an application of an engineered strain of mucor circinelloides using cellulose to efficiently synthesize gamma-linolenic acid, and belongs tothe technical fields of genetic engineering and biology. The strain construction method relates to simultaneous over-expression of delta 6 fatty acid desaturase (Delt-6) and cellulase (CBH2) genes inthe lipid-producing filamentous fungus mucor circinelloides. The obtained genetically engineered strain can use cellulose to efficiently synthesize the gamma-linolenic acid and provide guidance for vigorously promoting industrial production of the gamma-linolenic acid from a cellulose substrate by the mucor circinelloides.

The invention discloses a mucor circinelloides DF20, and the preservation number of the mucor circinelloides DF20 is CGMCC NO. 20742. The invention also discloses an application of the mucor circinelloides DF20, and the mucor circinelloides DF20 can be used for promoting the root development of salvia miltiorrhiza and the synthesis of tanshinone components. The strain DF20 an be used for effectively promoting the root system development of salvia miltiorrhiza and effectively promoting the biosynthesis and accumulation of tanshinone components through co-culture with salvia miltiorrhiza plants,and has a relatively high application value.

The invention relates to a method for preparing flax fiber by ultrasonic combination of a mucor circinelloides DK1 strain and hydrogen peroxide. The method comprises the following steps: (1) culturing the strain; (2) preparing a crude enzyme solution; and (3) mixing a flax raw material after sterilization and a mixed solution of the crude enzyme solution and hydrogen peroxide according to the mass-to-volume ratio of 1: (10-30), regulating a pH value, then putting into a shaking bed for treatment, finally adding sodium hydroxide, increasing the temperature, degumming, removing a degumming solution, washing, stopping washing when the pH value is natural, and completing degumming. The strain adopted by the invention is short in growth period, the strain is less liable to pollution, the treatment cost is low, and the degumming condition is mild; and the degumming method is simple in process flow, the time is shortened, the energy consumption is reduced, the pollution emission is reduced, and the method is conductive to environmental protection.

The invention relates to the field of biological medicines, and relates to a conversion method of a euphorbia lathyris diterpene compound. The method comprises the following steps of utilizing a known microorganism to perform microorganism conversion on the euphorbia lathyris phorbol and a derivative thereof, and combining with chemical acylation, so as to prepare a euphorbia lathyris diterpene derivative compound; especially, utilizing a mucor circinelloides (CICC40242) microorganism to perform hydroxylation biological conversion on the euphorbia lathyris phorbol and euphorbia factor L3, and performing chemical acylation on hydroxyls introduced by C8 and C18, so as to obtain three hydroxylated products and six C8 alcohol esterified products. The method has the advantages that the water solubility of the euphorbia factor L3 is improved by the hydroxylation at site, and the druggability is improved; furthermore, the prepared euphorbia lathyris diterpene compound can be used for preparing anti-tumor activity and anti-tumor multidrug resistance type pharmaceutical compositions. The chemical formula is shown in the specification.

The invention relates to a method for preparing laccase by using a mucor circinelloides DK1 strain. The method comprises the following steps: (1) transferring the mucor circinelloides DK1 strain from a PDA slant culture medium into a triangular flask filled with a basic liquid medium, carrying out shaking culture, filtering the fermentation liquor to obtain mycelium, washing the mycelium by using sterile water, scattering, collecting the mycelium in the sterile water to prepare mycelium suspension, and preserving the suspension for later use; (2) putting an enzyme production culture medium into the triangular flask, inoculating the mycelium suspension, carrying out shaking culture, periodically extracting the fermentation liquor in the culture process, centrifuging, and taking the supernatant, thereby obtaining the crude enzyme liquor; and (3) centrifuging the crude enzyme liquor, and taking the supernatant, thereby obtaining the laccase. The method disclosed by the invention is simple in process, is suitable for large-scale industrial production and the like, and the prepared laccase can effectively remove non-cellulose colloid in flax, jute, kenaf or bamboo fibers, has the advantages of high degumming efficiency, short degumming period, high fiber dispersion rate, high refined dried-jute quality and the like and has good application prospects.

The invention discloses an application of lactobacillus salivarius fermentation liquor as a feed mildew preventive, the lactobacillus salivarius fermentation liquor is prepared by fermenting lactobacillus salivarius preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus salivarius is CGMCC No.20700. The lactobacillus salivarius fermentation liquor has a good antibacterial effect on common feed mildew fungi such as aspergillus flavus, aspergillus oryzae, aspergillus niger, penicillium crust, mucor circinelloides, fusarium verticillioides and geotrichum candidum; the product can effectively delay the mildewing period and mildewing degree of feed and raw materials thereof, can effectively replace a traditional feed mildewproof agent, promotes the feed production industry and improves the meat quality of livestock and poultry.

The invention provides Mucor circinelloides which is preserved in China General Microbiological Culture Collection Center on April 9, 2021, with the preservation number of CGMCC No. 21479 and the classification name of Mucor circinelloides, wherein the preservation address is China General Microbiological Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, 3#, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing. The Mucor circinelloides can grow in a hydrothermal conversion aqueous phase product under proper conditions, and can convert multiple substances in hydrothermal conversion and reduce the toxicity of the hydrothermal conversion aqueous phase product.

The invention relates to a mucor circinelloides DK1 strain and an application thereof. An ITS (internal transcribed spacer) sequence of the strain is shown by SEQ ID NO:1. The strain is applied to preparation of flax, jute, kenaf or bamboo fiber by retting, boiling-off of flax, jute, kenaf or bamboo yarns, and degumming of flax, jute, kenaf original hemp or bamboo fabrics. The strain provided by the invention is short in growth cycle, cannot be polluted easily, is low in treatment cost, good in fiber quality after treatment, mild in treatment environment, good in heat resistance and free from environmental pollution; and the strain can be directly applied to degumming of the flax, jute, kenaf and bamboo fibers, and is short in degumming cycle, high in fiber dispersion rate and high in degumming efficiency.

The invention discloses an efficient oil-producing filamentous fungus mucor circinelloides Q-531 and an application thereof. The efficient oil-producing filamentous fungus is classified and named as mucor circinelloides Q-531 and is preserved in China Center for Type Culture Collection; the preservation number is CCTCC M2016364; the preservation date is on July 4th, 2016; the preservation address is at Wuhan University of China. According to the invention, a solid state fermentation process is adopted for directly converting mulberry branches into microbial oil; the highest oil yield can reach up to 42.43+ / -4.01mg / gds; the highest cellulase enzyme activity can reach up to 1.39+ / -0.09 FPU / gds. Besides, the highest oil content in fungus cell is 28.8+ / -2.85%. According to the invention, the mucor circinelloides research is utilized to produce oil by fermenting forestry and agricultural residues (mulberry branches) under solid state, so that the waste resources is reasonably utilized, the oil yield is increased, the production cost is reduced and the industrialization is benefited.

The invention discloses a method for producing microbial oil by using bacteria and enzymes to jointly ferment corn straw, and belongs to the field of microbial fermentation. The method utilizes the mutual synergistic effects of mucor circinelloides, yarrowia lipolytica and cellulase to ferment corn straw; and fermentation conditions are as follows: the adding amount of the cellulase is 1.5%; the strain ratio of the mucor circinelloides to the yarrowia lipolytica is 1: 1.5; total inoculation amount is 10%; the rotating speed of stirring is 200 rpm / min; fermentation temperature is 31 DEG C, andfermentation time is 54 h; and the fermentation broth of the corn straw per liter can obtain 1.24 g of microbial oil. The method can directly convert lignocellulose, so that the synchronization of cellulose-monosaccharide-oil can be realized in one step, therefore, production costs can be greatly reduced, and pollution to environment can be reduced.

The invention discloses an application of Mucor circinelloides in degradation of lambda-cyhalothrin. The invention aims to solve the technical problem that the application of the Mucor circinelloidesin degradation of the lambda-cyhalothrin is not recorded in the prior art. A liquid seed is prepared by using separated Mucor circinelloides, a lambda-cyhalothrin mother liquor is added under a sterile condition to prepare an isolation medium, expanded culture is carried out, and fermentation culture is carried out at a degradation fermentation temperature of 30 DEG C at a rotating speed of 120 rpm or above for 3-5 d. The Mucor circinelloides has a high lambda-cyhalothrin degrading characteristic and has the advantages of simple culture conditions, and rapidness in propagation, the lambda-cyhalothrin degrading rate is 79.7%, the lambda-cyhalothrin degrading capacity of the Mucor circinelloides is used to decompose pesticide residues, and the application has wide application values in the technical field of application of microbial strains.

The invention provides application of mucor circinelloides in gossypol degradation. Aiming at the technical status that in the prior art, there are no records about the application of the mucor circinelloides in the gossypol degradation, and the current status that cottonseed meal is used as the second largest plant protein resource of China, and in order to provide high-degradation-rate and stable-fermentation-property strains for the biological detoxification technology of cottonseed meal, the application of the mucor circinelloides in the gossypol degradation is provided. The mucor circinelloides has the advantages of being high in gossypol degradation rate, simple in culture condition and high in reproduction speed, the gossypol degradation rate is 67.5%, the cottonseed meal is detoxified by using the degradation ability of the strains to gossypol, and the application has a wide application value in the technical field of microbial strain application.

The invention discloses a method for allowing exogenous DNA to penetrate the cell walls and cell membranes of the ungerminated spores of mucor circinelloides. The method includes: culturing the mucor circinelloides and collecting the spores, pretreating the mucor circinelloides spores and using an HDEN method to perform electric shock on the mucor circinelloides spores to obtain the mucor circinelloides spores imported with to-be-transformed plasmids. The method has the advantages that the ungerminated spores are used as the initial materials for importing exogenous molecules, the HDEN electric transformation technology is used to import the exogenous DNA into the resting mucor circinelloides spores, the complex step of spore germination can be omitted, steps such as protoplast preparation or agrobacterium-mediated transformation in a traditional method are omitted, the method is high in transformation rate, and each transformation reaction system can at least reach the effect of not less than 6000 positive transformants.

Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. Δ15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and Δ15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-Δ15, finally, the expression of the Δ15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection Center on Jun. 20, 2018, with the address of No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The accession number given to the biological material by the collection center is CGMCC No. 15887, and the suggested taxonomic denomination is Mucor circinelloides-GLELO.