Method for producing microbial oil by using bacteria and enzymes to jointly ferment corn straw
A technology of microbial oil and fermented corn stalks, which is applied in the field of microbial fermentation to reduce environmental pollution and reduce production costs
Active Publication Date: 2020-07-14
SHANDONG UNIV OF TECH
View PDF4 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
At present, the oleaginous bacteria that are widely studied and used include Rhodosporidium toruloides, Cryptococcus, Trichoderma koningeni and Cellulomonas chrysogenum, and there is no joint production of corn straw by Mucor circinifolia and Yarrowia lipolytica. Report on microbial oil
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0015] Preparation of strains:
[0016] The strain Mucor circinelloides WJ11 preserved in our laboratory (Complete genome sequence of a high lipid-producing strain of Mucor circinelloides WJ11 and comparative genome analysis with a low lipid-producing strain CBS 277.49, PloS One, 2015, 10(9):e0137543) and Yarrowia lipolytica CICC1778 (Metabolic flux analysis of lipid biosynthesis in the yeast Yarrowia lipolytica using 13C-labled glucose and gaschromatography-mass spectrometry, Plos One, 2016, 11(7): e0159187).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more
Abstract
The invention discloses a method for producing microbial oil by using bacteria and enzymes to jointly ferment corn straw, and belongs to the field of microbial fermentation. The method utilizes the mutual synergistic effects of mucor circinelloides, yarrowia lipolytica and cellulase to ferment corn straw; and fermentation conditions are as follows: the adding amount of the cellulase is 1.5%; the strain ratio of the mucor circinelloides to the yarrowia lipolytica is 1: 1.5; total inoculation amount is 10%; the rotating speed of stirring is 200 rpm / min; fermentation temperature is 31 DEG C, andfermentation time is 54 h; and the fermentation broth of the corn straw per liter can obtain 1.24 g of microbial oil. The method can directly convert lignocellulose, so that the synchronization of cellulose-monosaccharide-oil can be realized in one step, therefore, production costs can be greatly reduced, and pollution to environment can be reduced.
Description
technical field [0001] The invention relates to a method for producing microbial oil by bacterial enzyme co-fermentation of corn stalks, which belongs to the field of microbial fermentation. The fermenting bacteria enzyme of the present invention relates to Mucor circinosa, Yarrowia lipolytica and cellulase. Background technique [0002] With the increasing shortage of fossil resources, it is the general trend to develop renewable oil resources through multiple channels. Oleaginous microorganisms can accumulate a large amount of triglycerides in cells, and it is inevitable for sustainable development to produce oil through microbial transformation of renewable resources and partially replace fossil resources. In addition, a small number of fungi, microalgae, bacteria, etc. can also synthesize functional polyunsaturated fatty acids that are very beneficial to human health, such as arachidonic acid (Arachidonic acid), γ-linolenic acid (γ-linolenic acid), docosan Docosahexaen...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more
IPC IPC(8): C12P39/00C12P19/14C12P7/64C12N1/16C12N1/14C12R1/785C12R1/645
CPCC12P39/00C12P19/14C12P7/6463C12N1/16C12N1/14C12P2201/00Y02E50/10
Inventor 张瑶宋元达王艳霞
Owner SHANDONG UNIV OF TECH
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap