The invention relates to a macroelement water-soluble fertilizer (medium-trace element comprehensive) integrating full nutrition, continuous-cropping resistance and disease resistance, and a preparation method thereof, and belongs to the technical field of fertilizers, wherein the macroelement water-soluble fertilizer is prepared from the following raw materials: 5-10% of urea, 15-20% of potassiumdihydrogen phosphate, 40-50% of potassium nitrate, 5-10% of monoammonium phosphate, 5-10% of potassium fulvate, 2-5% of calcium ammonium nitrate, 1-3% of magnesium nitrate, 2-5% of magnesium sulfate,1.0-1.5% of boric acid, 0.2-0.3% of ammonium molybdate, 1.5-2% of ferrous sulfate, 0.5-1% of zinc sulfate, 0.5-1% of copper sulfate, 0.5-1% of manganese sulfate, 2-3% of amino acid powder, 1-1.5% ofchitin, 0.3-0.5% of compound sodium nitrophenolate, 0.2-0.3% of diethyl aminoethyl hexanoate, 0.2-0.3% of agricultural streptomycin, and 0.1-0.2% of Bacillus subtilis. According to the present invention, the macroelement water-soluble fertilizer is creatively prepared, is firstly provided in the patents related to water-soluble fertilizers, has characteristics of full nutrition and continuous-cropping resistance, can solve the deficiency and the physiological diseases of crops, further has advantages of yield improving, quality improving and environmental protection, easily achieves the development of ecological agriculture, and fills the domestic blank.