Nutrient solution special for tobacco floating seedling raising and preparation method thereof

Abstract

The invention belongs to the technical field of seedling culture. The invention provides a special nutrient solution for floating seedling raising of tobacco. The nutrient solution comprises the following components: 1000g of water, 0.3-0.5 g of acorus calamus and goldhair hedyotis herb aqueous extract fermented product, 0.1-0.3 part of corn straw steam explosion enzymolysis fermented product, 0.2-0.4 g of calcium ammonium nitrate, 0.6-0.8 g of potassium sulfate, 0.3-0.5 g of ammonium bicarbonate, 0.3-0.5 part of ammonium dihydrogen phosphate, 1-2mg of boric acid and 1-2mg of zinc sulfate. The nutrient solution special for tobacco floating seedling raising can remarkably improve the emergence rate of floating seedling raising, improve the seedling strengthening index and greatly reduce the disease index of tobacco infected with black shank; when the special nutrient solution for tobacco floating seedling raising is prepared, the used technical treatment means are mature technologies in the industry, so that the special nutrient solution has higher feasibility and high industrial application value.

Description

Technical Field

[0001] This invention belongs to the field of seedling technology, specifically relating to a special nutrient solution for tobacco floating seedling cultivation and its preparation method. Background Technology

[0002] As an important component of modern tobacco agriculture, floating seedling cultivation technology directly affects the growth, development, and transplanting quality of tobacco seedlings through its nutrient solution formulation and management techniques. In recent years, researchers have conducted extensive innovative work focusing on the optimization of nutrient solution components, concentration control, application techniques, and biofortification.

[0003] Traditional nutrient solutions mainly contain nitrogen, phosphorus, and potassium, with a ratio typically controlled within the range of 1:0.5 to 1:1.5. This ratio has been verified through long-term practice and can meet the basic growth needs of tobacco seedlings. However, with in-depth research, it has been found that simple formulas containing only N, P, and K are insufficient to meet the comprehensive nutritional needs for healthy tobacco seedling growth, especially in terms of significantly insufficient disease resistance.

[0004] Currently, there is limited research on enhancing tobacco disease resistance through the development of nutrient solutions. Whether and how to develop specialized floating seedling nutrient solutions to promote germination, seedling vigor, and improve tobacco's resistance to diseases is a topic worthy of further research. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a special nutrient solution for tobacco floating seedling cultivation, which can promote seedling emergence and strong seedlings and significantly enhance the tobacco's resistance to disease.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A nutrient solution specifically for floating tobacco seedling cultivation, the nutrient solution comprising the following components:

[0008] 1000g water, 0.8-1.2g fermented water extract of Acorus calamus and Aristolochia debilis, 0.3-0.5 parts steam-explosion enzymatic hydrolysis fermented corn straw, 0.2-0.4g calcium ammonium nitrate, 0.6-0.8g potassium sulfate, 0.3-0.5g ammonium bicarbonate, 0.3-0.5 parts ammonium dihydrogen phosphate, 1-2mg boric acid, 1-2mg zinc sulfate;

[0009] The preparation method of the fermentation pyrolysis product of the aqueous extract of Acorus calamus and Aristolochia debilis is as follows: Acorus calamus and Aristolochia debilis are used as extraction substrates and water is used as extraction agent at a weight ratio of 3 to 5:1. After separation, the residue phase is obtained. Then, the obtained residue phase is used as fermentation substrate and Bacillus subtilis B115 is used as fermentation bacteria for fermentation to obtain the product.

[0010] The corn stalks are processed by steam explosion, followed by enzymatic hydrolysis with hemicellulase, and then separated by washing to obtain the residue phase. The residue phase is then used as the fermentation substrate, and Bacillus subtilis B115 is used as the fermentation bacteria for fermentation.

[0011] The Bacillus subtilis B115 has the accession number CGMCC NO.1210.

[0012] This invention is a further development based on another research result of the inventors (for which a separate patent application has been filed). This other research result discovered that the pyrolysis complex of manure-plant extract residue can be used as a substrate material for floating seedling cultivation, not only promoting germination and seedling vigor but also improving resistance to black shank disease. Encouraged by this research, the inventors conducted studies on using the pyrolysis complex of manure-plant extract residue as a nutrient solution component. Unfortunately, although it can reduce the disease index of black shank disease to some extent, the reduction is not significant, and its impact on germination rate is not prominent. Existing research has rarely addressed how to improve crop resistance to blight during the floating seedling stage. Therefore, there is no credible theory to explain this experimental phenomenon. The inventors initially consider that the reason for this result may be that different substrate materials and differences in crop absorption of related components may affect crop disease resistance.

[0013] Although directly adding manure-plant extract pyrolysis compound to conventional nutrient solutions did not significantly improve crop resistance to black shank, and the addition of special raw materials was insufficient to generate an acceptable economic return, it is encouraging that the addition of manure-plant extract pyrolysis compound at least produced a positive effect. Following this line of thought, the inventors conducted a series of exploratory studies.

[0014] In another research finding, the inventors utilized calamus by extracting its water-soluble components from the liquid phase using a water extraction method. These components were then mixed with the fermentation products of a mixture of chicken manure, cow manure, and the residue from the water extract of *Acorus calamus*, and subsequently processed using a pyrolysis method. Due to time constraints, the inventors have not yet conducted testing and analysis of the relevant components in the resulting products. However, based on general chemical knowledge, it is known that fermentation will produce corresponding metabolites. Furthermore, after pyrolysis, the metabolites and flavonoids in the water extract will decompose into smaller molecules. These substances may be beneficial in enhancing crop resistance to disease and are more easily absorbed by crops.

[0015] The inventors conducted a series of studies on different extracts of calamus and golden ear fungus, combined with fermentation technology. They finally discovered that using calamus and golden ear fungus as water extraction substrates, then fermenting the water extraction residue, and then combining it with corn straw steam explosion enzymatic hydrolysis fermentation product as a nutrient solution for tobacco floating seedling cultivation, can achieve similar technical effects to another research achievement of the inventors. That is, it can promote seedling emergence and strong seedlings, and also improve the crop's resistance to black shank disease.

[0016] By comparing this invention with another research result of the inventor, it is easy to find that the nutrient solution of this invention not only contains fermentation products but also live Bacillus subtilis B115 bacteria, while the other research result, due to high-temperature pyrolysis, yielded products without live bacteria, and the metabolites underwent corresponding changes at high temperatures. In this technical field, there are many reports that related microbial agents can improve the floating seedling raising effect of tobacco. For example, CN101786912A found that Bacillus sp. A03, as a substrate material, can not only strengthen seedlings but also improve resistance to blight. However, similar microbial fertilizers are difficult to find. There are currently no reports of Bacillus subtilis B115 bacteria used in this invention being used as a seedling microbial fertilizer. Moreover, as the experimental results of this invention show, adding Bacillus subtilis B115 bacteria alone to the seedling substrate did not improve the resistance to black shank disease. At the same time, as shown in the comparative examples of this invention, after sterilizing the fermentation product at 121°C, the nutrient solution obtained also had a limited effect on improving the crop's resistance to black shank disease. Therefore, it is preliminarily believed that live Bacillus subtilis B115, fermented water extracts of Acorus calamus and Acorus gramineus, and fermented steam-explosion enzymatic hydrolysis of corn straw have a synergistic effect in improving crop disease resistance.

[0017] Preferably, in preparing the fermented pyrolysis product of the water extract of Acorus calamus and Aristolochia debilis, the weight ratio of the extract substrate to water is 1:10; when inoculating Bacillus subtilis B115 for fermentation, the inoculation amount is 2% and the fermentation time is 24 hours.

[0018] Preferably, during the steam explosion, the crushed corn stalks are introduced into saturated steam and treated at 1.2 MPa for 5 minutes.

[0019] Preferably, the enzymatic hydrolysis is performed at a temperature of 30°C for 1 hour.

[0020] Preferably, in preparing the corn straw steam explosion enzymatic hydrolysis fermentation product, the inoculum amount of Bacillus subtilis B115 is 2%, and the fermentation time is 24 hours.

[0021] Preferably, during the fermentation and pyrolysis of the water extract of calamus and golden ear grass, the weight ratio of calamus to golden ear grass is 4:1.

[0022] Preferably, the extraction temperature is 80°C during the fermentation and pyrolysis of the aqueous extracts of Acorus calamus and Aristolochia debilis.

[0023] Preferably, the extraction time for the fermentation pyrolysis product of the water extract of calamus and golden ear grass is 2 hours.

[0024] Preferably, the nutrient solution comprises the following components:

[0025] 1000g water, 0.4g fermented water extract of Acorus calamus and Aristolochia debilis, 0.2 parts steam-explosion enzymatic hydrolysis fermented corn stalks, 0.3g calcium ammonium nitrate, 0.8g potassium sulfate, 0.3g ammonium bicarbonate, 0.4 parts ammonium dihydrogen phosphate, 2mg boric acid, 2mg zinc sulfate.

[0026] A method for preparing a special nutrient solution for tobacco floating seedling cultivation, wherein the preparation method involves mixing the components of the aforementioned nutrient solution.

[0027] The beneficial effects of this invention are:

[0028] The tobacco floating seedling special nutrient solution obtained by this invention can significantly improve the germination rate of floating seedlings, increase the seedling vigor index, and greatly reduce the disease index of tobacco infected with black shank. The technical processing methods used in the preparation of the tobacco floating seedling special nutrient solution are all mature technologies in the industry, with high feasibility and high industrial application value. Detailed Implementation

[0029] The present invention will be specifically described below through embodiments. It should be noted that the following embodiments are only used to further illustrate the present invention and should not be construed as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above-described invention are still within the scope of protection of the present invention.

[0030] Unless otherwise specified, the raw materials and products used in the following examples and preliminary experimental cases are as follows:

[0031] Ammonium bicarbonate: purchased from Chengdu Sange Chemical Co., Ltd.;

[0032] Calcium ammonium nitrate: Guangxi Zhuangda Fertilizer Co., Ltd.;

[0033] Potassium sulfate: Kunming Shahaobei Trading Co., Ltd.;

[0034] Ammonium dihydrogen phosphate: Jinan Xinchenxu Chemical Co., Ltd.;

[0035] Boric acid and zinc sulfate: both are available in the laboratory.

[0036] Corn stalks: collected from local cornfields;

[0037] Bacillus subtilis B115, with accession number CGMCC NO.1210, is a publicly disclosed strain and was purchased from the China General Microbiological Culture Collection Center.

[0038] Hemicellulase: Henan Yangcheng Chemical Products Co., Ltd., brand name "Yangcheng";

[0039] Tobacco seeds: Yunyan 87;

[0040] Example 1

[0041] 1. Preparation of fermented aqueous extracts of Acorus calamus and Acorus gramineus:

[0042] Acorus calamus and Achyranthes bidentata were used as extraction substrates at a weight ratio of 4:1, and water was used as the extraction solvent. The weight ratio of extraction substrate to water was 1:10. The extraction was carried out at 80°C for 2 hours. The extract was then subjected to solid-liquid separation to obtain the residue phase. The obtained residue phase was then used as the fermentation substrate, and Bacillus subtilis B115 was inoculated at an inoculum amount of 2%. Fermentation was carried out at 35°C for 24 hours to obtain the final product.

[0043] 2. Preparation of corn stalk steam explosion enzymatic hydrolysis fermentation product:

[0044] The crushed corn stalks were passed through saturated steam and treated at 1.2 MPa for 5 minutes; then placed in a hemicellulase aqueous solution (800 IU / mL) and treated at 30°C for 1 hour. After rinsing with water, Bacillus subtilis B115 was inoculated at an inoculation rate of 2% and fermented at 35°C for 24 hours to obtain the final product.

[0045] 3. Mix the following components by weight:

[0046] 1000g water, 0.4g fermented water extract of Acorus calamus and Aristolochia debilis, 0.2 parts steam-explosion enzymatic hydrolysis fermented corn stalks, 0.3g calcium ammonium nitrate, 0.8g potassium sulfate, 0.3g ammonium bicarbonate, 0.4 parts ammonium dihydrogen phosphate, 2mg boric acid, 2mg zinc sulfate.

[0047] Example 2

[0048] 1. Preparation of fermented aqueous extracts of Acorus calamus and Acorus gramineus:

[0049] Acorus calamus and Achyranthes bidentata were used as extraction substrates at a weight ratio of 3:1, and water was used as the extraction solvent. The weight ratio of extraction substrate to water was 1:10. The extraction was carried out at 80°C for 2 hours. The extract was then subjected to solid-liquid separation to obtain the residue phase. The obtained residue phase was then used as the fermentation substrate, and Bacillus subtilis B115 was inoculated at an inoculum amount of 2%. Fermentation was carried out at 35°C for 24 hours to obtain the final product.

[0050] 2. Preparation of corn stalk steam explosion enzymatic hydrolysis fermentation product:

[0051] The crushed corn stalks were passed through saturated steam and treated at 1.2 MPa for 5 minutes; then placed in a hemicellulase aqueous solution (800 IU / mL) and treated at 30°C for 1 hour. After rinsing with water, Bacillus subtilis B115 was inoculated at an inoculation rate of 2% and fermented at 35°C for 24 hours to obtain the final product.

[0052] 3. Mix the following components by weight:

[0053] 1000g water, 0.5g fermented water extract of calamus and golden ear grass, 0.1 part steam explosion enzymatic hydrolysis fermented corn straw, 0.4g calcium ammonium nitrate, 0.6g potassium sulfate, 0.5g ammonium bicarbonate, 0.5 part ammonium dihydrogen phosphate, 1mg boric acid, 1mg zinc sulfate.

[0054] Example 3

[0055] 1. Preparation of fermented aqueous extracts of Acorus calamus and Acorus gramineus:

[0056] Acorus calamus and Achyranthes bidentata were used as extraction substrates at a weight ratio of 5:1, and water was used as the extraction solvent. The weight ratio of extraction substrate to water was 1:10. The extraction was carried out at 80°C for 2 hours. The extract was then subjected to solid-liquid separation to obtain the residue phase. The obtained residue phase was then used as the fermentation substrate, and Bacillus subtilis B115 was inoculated at an inoculum amount of 2%. Fermentation was carried out at 35°C for 24 hours to obtain the final product.

[0057] 2. Preparation of corn stalk steam explosion enzymatic hydrolysis fermentation product:

[0058] The crushed corn stalks were passed through saturated steam and treated at 1.2 MPa for 5 minutes; then placed in a hemicellulase aqueous solution (800 IU / mL) and treated at 30°C for 1 hour. After rinsing with water, Bacillus subtilis B115 was inoculated at an inoculation rate of 2% and fermented at 35°C for 24 hours to obtain the final product.

[0059] 3. Mix the following components by weight:

[0060] 1000g water, 0.3g fermented water extract of Acorus calamus and Acorus gramineus, 0.3 parts steam-explosion enzymatic hydrolysis fermented corn stalks, 0.2g calcium ammonium nitrate, 0.7g potassium sulfate, 0.4g ammonium bicarbonate, 0.3 parts

[0061] Ammonium dihydrogen phosphate, 1 mg boric acid, 1 mg zinc sulfate.

[0062] Comparative Example 1

[0063] Compared with Example 1, in this invention, after preparing the aqueous extract fermentation product of Acorus calamus and Aristolochia debilis and the steam explosion enzymatic hydrolysis fermentation product of corn straw, the obtained fermentation products were all subjected to high-temperature sterilization at a temperature of 121°C for 20 minutes. The rest is the same as in Example 1.

[0064] Comparative Example 2

[0065] Compared with Example 1, this comparative example only involves fermentation treatment of corn stalks, without steam explosion and enzymatic hydrolysis treatment, and the rest is the same as Example 1.

[0066] Comparative Example 3

[0067] Compared with Example 1, this comparative example does not add corn straw steam explosion enzymatic hydrolysis fermentation product, but is otherwise the same as Example 1.

[0068] Experimental Example 1

[0069] This invention is based on another research result of the inventor, and focuses on the use of the pyrolysis complex of manure plant extract residue obtained from that result as a nutrient solution additive.

[0070] 1. Preparation of pyrolysis complex of manure and plant extract residue:

[0071] Golden ear grass and calamus: sourced from local Chinese medicinal herb planting base, are fresh whole herbs that have not been processed;

[0072] Bacillus subtilis B115, with accession number CGMCC NO.1210, is a publicly disclosed strain and was purchased from the China General Microbiological Culture Collection Center.

[0073] Water extract residue of *Hypericum glabra*: Crush the whole *Hypericum glabra* herb (crushed to an average diameter of 0.3-0.5 cm), add it to water at a weight ratio of 1:10, extract at 80°C for 2 hours, filter and collect the residue to obtain the water extract residue of *Hypericum glabra*.

[0074] Aqueous extract of calamus: The whole calamus herb is crushed (crushed to an average diameter of 0.3-0.5 cm), added to water at a weight ratio of 1:10, extracted at 80°C for 2 hours, and the filtrate is obtained by filtration.

[0075] The preparation method of the pyrolysis complex of manure and plant extract residue is as follows:

[0076] (1) Chicken manure, cow manure and water extract residue of golden ear grass were mixed in a weight ratio of 7:3:3 and the moisture content was adjusted to 20%. Then, Bacillus subtilis B115 was inoculated at a 5% inoculation rate for fermentation for 12 hours. The preservation number of Bacillus subtilis B115 was CGMCC NO.1210.

[0077] (2) Mix the water extract of calamus with the product obtained in step (1) at a volume ratio of 4:10, and pyrolyze at 250℃ and 5MPa for 30 minutes, and take the solid phase.

[0078] 2. Preparation of nutrient solution:

[0079] The aforementioned pyrolysis complex of manure plant extract residue was mixed with commercially available seedling fertilizer (purchased from Sichuan Jinye Fertilizer Co., Ltd.) at a weight ratio of 3:7.

[0080] 3. Seedling substrate:

[0081] Prepare a conventional seedling substrate in this field: mix peat moss, vermiculite, and expanded perlite in a volume ratio of 3:1:1.

[0082] 4. Test Procedure:

[0083] Nutrient solutions containing pyrolysis compound of manure and plant extract residue were used as the experimental group, while nutrient solutions without the pyrolysis compound of manure and plant extract residue were used as the control group. The following tests were conducted.

[0084] (1) Seedling emergence rate and seedling vigor index test

[0085] The floating seedling raising technique was carried out in accordance with the "Technical Specification for Intensive Tobacco Seedling Raising" (GB / T 25241.1—2010). The nutrient solution used was prepared using Xinye brand special seedling fertilizer produced by Sichuan Jinye Fertilizer Co., Ltd.

[0086] The germination rate was investigated 7, 9, and 11 days after sowing.

[0087] After the seedling stage, 20 tobacco seedlings of similar growth and development were randomly selected from the seedling trays of each treatment group. After cleaning and removing impurities, the seedlings were dried with absorbent paper. The above-ground and underground parts of the seedlings were separated, and their fresh weight, stem diameter, and plant height were measured. After drying, the seedlings were weighed, and the seedling vigor index was calculated using the following formula:

[0088] Strong seedling index = (stem diameter / plant height + root dry weight / aboveground dry weight) × total plant dry weight.

[0089] (2) Blackleg disease resistance test

[0090] After the tobacco seedlings were pruned once (seedling age 40), 60 seedlings were selected from each group and transplanted into new floating trays. The preserved black shank strain was activated, inoculated into oat liquid medium, and cultured in a shaker at 28℃ and 150 rpm for 2 weeks. A 0.1% potassium nitrate solution was added to prepare a solution with a concentration of 1×10⁻⁶. 8A suspension of zoospores at cfu / mL was prepared. The zoospore suspension was inoculated using the stem base wound inoculation method. After inoculation, the seedling trays were placed in a constant temperature tank at 28℃, and water was sprayed twice daily to maintain high humidity and induce disease. Disease incidence and disease index were calculated starting 5 days after inoculation and repeated every 3 days.

[0091] The experimental results are shown in Table 1-3.

[0092] Table 1

[0093]

[0094] Table 2

[0095]

[0096] Table 3

[0097]

[0098] As shown in Tables 1-3, the addition of fecal plant extract residue pyrolysis compound to commercially available nutrient solutions did not significantly reduce the disease index or promote the seedling emergence rate.

[0099] Experiment Example 2

[0100] This experimental example examines the fermentation broth of Bacillus subtilis B115 (1×10⁻⁶ cells / mL). 8 The bacterial fermentation broth (cfu / mL) was added to the seedling substrate used in Experiment 1 (addition ratio of 1% wt), and its effect on the resistance of floating tobacco seedlings to black shank was investigated. The experimental group used the fermentation broth with added bacteria, while the control group used the fermentation broth without added bacteria. The relevant experimental protocol is the same as in Experiment 1.

[0101] The experimental results are shown in Table 4.

[0102] Table 4

[0103]

[0104] As shown in Table 4, the fermentation broth of Bacillus subtilis B115 did not reduce the severity index of black shank disease.

[0105] Experimental Example 3

[0106] This experiment investigated the effects of the nutrient solutions obtained in Examples 1-3 and Comparative Examples 1-3 on the germination rate, seedling vigor index, and resistance to black shank disease in tobacco floating seedling cultivation. All experiments used the seedling substrate from Experiment 1. Furthermore, the relevant experimental protocols were referenced from Experiment 1.

[0107] The experimental results are shown in Table 5-7.

[0108] Table 5

[0109] Table 6

[0110]

[0111] Table 7

[0112]

Claims

1. A special nutrient solution for tobacco floating seedling cultivation, characterized in that, The nutrient solution comprises the following components: 1000g water, 0.3-0.5g fermented water extract of Acorus calamus and Aristolochia debilis, 0.1-0.3 parts steam-explosion enzymatic hydrolysis fermented corn straw, 0.2-0.4g calcium ammonium nitrate, 0.6-0.8g potassium sulfate, 0.3-0.5g ammonium bicarbonate, 0.3-0.5 parts ammonium dihydrogen phosphate, 1-2mg boric acid, 1-2mg zinc sulfate; The preparation method of the fermentation pyrolysis product of the aqueous extract of Acorus calamus and Aristolochia debilis is as follows: Acorus calamus and Aristolochia debilis are used as extraction substrates and water is used as extraction agent at a weight ratio of 3 to 5:

1. After separation, the residue phase is obtained. Then, the obtained residue phase is used as fermentation substrate and Bacillus subtilis B115 is used as fermentation bacteria for fermentation to obtain the product. The corn stalk steam explosion enzymatic hydrolysis fermentation product is obtained by treating corn stalks with steam explosion, then enzymatically hydrolyzing them with hemicellulase, washing and separating the residue phase, and then using the obtained residue phase as the fermentation substrate and Bacillus subtilis B115 as the fermentation bacteria for fermentation. The Bacillus subtilis B115 has the accession number CGMCC NO.1210.

2. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, In preparing the fermentation pyrolysis product of the aqueous extract of Acorus calamus and Aristolochia debilis, the weight ratio of the extract substrate to water was 1:10; when inoculating Bacillus subtilis B115 for fermentation, the inoculation amount was 2% and the fermentation time was 24 hours.

3. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, During the steam explosion, the crushed corn stalks are introduced into saturated steam and treated at 1.2 MPa for 5 minutes.

4. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, The enzymatic hydrolysis was carried out at a temperature of 30°C for 1 hour.

5. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, In preparing the corn straw steam explosion enzymatic hydrolysis fermentation product, the inoculum amount of Bacillus subtilis B115 was 2%, and the fermentation time was 24 hours.

6. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, During the fermentation and pyrolysis of the aqueous extracts of Acorus calamus and Aristolochia debilis, the weight ratio of Acorus calamus to Aristolochia debilis was 4:

1.

7. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, During the fermentation and pyrolysis of the aqueous extracts of Acorus calamus and Aristolochia debilis, the extraction temperature was 80℃.

8. The nutrient solution for tobacco floating seedling cultivation according to claim 7, characterized in that, During the fermentation and pyrolysis of the aqueous extracts of Acorus calamus and Aristolochia debilis, the extraction time was 2 hours.

9. The nutrient solution for tobacco floating seedling cultivation according to claim 1, characterized in that, The nutrient solution comprises the following components: 1000g water, 0.4g fermented water extract of Acorus calamus and Aristolochia debilis, 0.2 parts steam-explosion enzymatic hydrolysis fermented corn stalks, 0.3g calcium ammonium nitrate, 0.8g potassium sulfate, 0.3g ammonium bicarbonate, 0.4 parts ammonium dihydrogen phosphate, 2mg boric acid, 2mg zinc sulfate.

10. A method for preparing a special nutrient solution for tobacco floating seedling cultivation, characterized in that, The preparation method involves mixing the components of the nutrient solution according to any one of claims 1 to 9.

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