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Method for preparing laccase by using mucor circinelloides DK1 strain

A technology of Mucor circinigii and bacterial strains, which is applied in the field of preparing laccase by Mucor circinzia DK1 strain, can solve the problems of high energy consumption in the chemical degumming process, waste water cannot be recycled, and large equipment loss, etc., and achieves good application prospects and degumming High efficiency and good quality effect

Inactive Publication Date: 2015-05-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scouring method commonly used in the hemp industry is mainly the chemical method, that is, the raw hemp or roving is treated with strong acid and alkali. The chemical degumming process consumes a lot of energy and equipment loss, and the discharged wastewater cannot be recycled, causing serious pollution problems.

Method used

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  • Method for preparing laccase by using mucor circinelloides DK1 strain
  • Method for preparing laccase by using mucor circinelloides DK1 strain
  • Method for preparing laccase by using mucor circinelloides DK1 strain

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The method for preparing laccase by Mucor circiniferus DK1 strain:

[0027] (1) Mycelia suspension: Mucor circiniferatum DK1 strain was transferred from PDA slant medium into a 300mL Erlenmeyer flask containing 50mL of basic liquid medium, and cultured in a shaker at 180rpm at a constant temperature of 30°C for 3 days, then fermented The liquid was filtered through sterile gauze, the mycelium was washed with sterile water and dispersed in a Waring high-speed crushing cup for 15 seconds, collected in sterile water (equal to the volume of the fermentation broth) to make a suspension of mycelia, and collected at 40 Store at ℃ for later use.

[0028] Basic liquid medium: 200g potato, 20g glucose, 1000mL distilled water, natural pH.

[0029] (2) Enzyme production in shake flasks: add 1% (w / v) natural substrate) and different concentrations of CuSO in the base liquid medium 4 ·5H 2 O (0-4mM) is the enzyme production medium. Put 80mL of enzyme-producing medium into a 300mL...

Embodiment 2

[0033] The degumming process of flax, jute, kenaf or bamboo fiber prepared by the laccase crude enzyme solution prepared by Mucor circini DK1 is as follows:

[0034] Put 5g of flax, jute, kenaf or bamboo fiber raw stems in a 250ml conical flask, and use flax, jute, kenaf or bamboo fiber raw stems and laccase crude enzyme solution in a mass ratio of 1:20 to pack in conical flasks 100ml liquid, degumming at 40°C, 200rpm shaker for 1 day, 2 days and 4 days respectively; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5min, rinse with tap water several times, and Remove bacteria from linen and stop degumming.

[0035] Through the observation of flax, jute, kenaf or bamboo fiber degumming, it was found that after 1 day, the degumming of flax, jute, kenaf or bamboo fiber was obviously dispersed, and the color of the fermentation liquid became dark and turbid; , kenaf or bamboo fibers are dispersed into fibers, and the fermented liquid ...

Embodiment 3

[0037] The process of preparing laccase enzyme solution for degumming in flax, jute or kenaf yarn by Mucor circinzia DK1 is as follows:

[0038]Put 5g of flax, jute or kenaf yarn into a 250ml conical flask, and divide flax, jute or kenaf yarn and laccase enzyme solution into the conical flask according to the mass ratio of 1:20, and the liquid volume is 100ml , degumming at 40°C, 200rpm shaker for 1 day, 2 days and 4 days respectively; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5min, and rinse with tap water several times to remove flax, jute or kenaf Bacteria on yarn, stop degumming.

[0039] Through the observation of flax, jute or kenaf yarn degumming, it was found that the flax fiber was obviously dispersed after 1 day, and the color of the fermentation liquid became turbid; Days later, the flax degumming rate reached over 90%.

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Abstract

The invention relates to a method for preparing laccase by using a mucor circinelloides DK1 strain. The method comprises the following steps: (1) transferring the mucor circinelloides DK1 strain from a PDA slant culture medium into a triangular flask filled with a basic liquid medium, carrying out shaking culture, filtering the fermentation liquor to obtain mycelium, washing the mycelium by using sterile water, scattering, collecting the mycelium in the sterile water to prepare mycelium suspension, and preserving the suspension for later use; (2) putting an enzyme production culture medium into the triangular flask, inoculating the mycelium suspension, carrying out shaking culture, periodically extracting the fermentation liquor in the culture process, centrifuging, and taking the supernatant, thereby obtaining the crude enzyme liquor; and (3) centrifuging the crude enzyme liquor, and taking the supernatant, thereby obtaining the laccase. The method disclosed by the invention is simple in process, is suitable for large-scale industrial production and the like, and the prepared laccase can effectively remove non-cellulose colloid in flax, jute, kenaf or bamboo fibers, has the advantages of high degumming efficiency, short degumming period, high fiber dispersion rate, high refined dried-jute quality and the like and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological textiles, and in particular relates to a method for preparing laccase from Mucor circinifolia DK1 strain. Background technique [0002] Laccase (Laccase, EC.1.10.3.2) is a copper-containing polyphenol oxidase, which belongs to the same family of ceruloplasmin oxidases as ascorbate oxidase in plants and ceruloplasmin in mammalian plasma. Due to its high redox potential, fungal laccase can act on a wide range of substrates, and can catalyze the oxidation of various phenols and aromatic amines, while reducing molecular oxygen to water. The outstanding catalytic properties of laccase are that its substrates are extensive, the catalytic reaction is complex, and the products produced are environmentally friendly. Therefore, in recent years, the use of fungi to prepare laccase in the degumming of hemp fiber has become more and more extensive. . [0003] There are many kinds of hemp fibers, which are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12R1/785
CPCC12N9/0061C12Y110/03002
Inventor 丁若垚郁崇文张兴群郭营李晓龙关赛鹏
Owner DONGHUA UNIV
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