Mucor circinelloides FNZJ3-2-2 resistant to aluminum profile electronickelling bath solution and application of mucor circinelloides FNZJ3-2-2
A technology of Mucor circinifolia and nickel plating, applied in the direction of microorganism-based methods, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of complex heavy metal components, waste of resources, pollution and hazards, etc. Achieve good application prospects, excellent nickel enrichment ability and growth activity
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Embodiment 1
[0028] Example 1: Isolation, purification and identification of Mucor circiniferus FNZJ3-2-2
[0029] 1. Separation and purification
[0030] Enrichment culture: take the activated sludge (moisture content is 16.94 ± 1.47%, pH is 5.09 ± 0.21, soil organic matter is 304.85 ± 6.95g / kg, total nitrogen 41.90±3.10g / kg, total phosphorus 24.95±2.05g / kg), packaged in aseptic bags and stored in a 4°C refrigerator. Diluted with distilled water to 1 times, 1 / 2 times, 1 / 3 times, 1 / 4 times, 1 / 5 times, 1 / 10 times and 1 / 20 times of 100mL electroplating nickel bath solution for aluminum profiles and collected activity Put about 10L of sludge together into a 250mL Erlenmeyer flask equipped with glass beads. In addition, use double distilled water to make a comparison in the same way. Carry out aerobic culture and anaerobic culture sealed in liquid paraffin liquid in a constant temperature shaker at 28°C and 160r / min for 7-15 days to obtain enriched culture solution.
[0031] Preliminary scr...
Embodiment 2
[0041] Embodiment 2: the electroplating nickel bath solution treatment effect of aluminum profiles under aerobic and anaerobic conditions
[0042] Take 1000ml of electroplating nickel bath solution for aluminum profiles, and add K to it successively 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 NO 3 1.05g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.001g, adjust the pH to 4.2-4.5 with 1mol / L HCl to obtain a fermentation broth.
[0043] The Mucor circinifolia FNZJ3-2-2 that embodiment 1 obtains is diluted into the bacterium suspension of appropriate concentration with sterile water, in the fermented culture liquid of inoculation 100mL, the inoculum size that makes Mucor circinatus FNZJ3-2-2 is 3.16×10 7 strains / ml fermentation broth, and then cultured in a water bath constant temperature shaker at 28°C for 4 days. The culture is divided into aerobic group and anaerobic group, wherein the aerobic group is 160r / min shaking culture, the bottled volume of the culture solu...
Embodiment 3
[0048] Example 3 investigates the treatment effect of aluminum profile nickel plating bath solution with different inoculum amounts of Mucor circiniferus FNZJ3-2-2
[0049] Substantially the same as Example 2, the difference is that the inoculum size of Mucor circiniferus FNZJ3-2-2 was investigated to be 1.0 × 10 7 strain / ml fermentation broth, 2.0×10 7 strain / ml fermentation broth, 4.0×10 7 strain / ml fermentation medium, 5.0×10 7 strains / ml fermentation broth, 8.0×10 7 The treatment of aluminum profile nickel-plating bath solution when each strain / ml fermentation culture solution; the culture condition is 28°C, 160r / min; the bottled volume of the culture solution is 35%.
[0050] After cultivating for 4 days, the bacteria content and nickel content in the culture solution were measured, and the nickel-rich rate (%) was calculated. The results are shown in Table 3.
[0051] The test results of Mucor circinelloides FNZJ3-2-2 fermented and cultivated for 4 days with differen...
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